Berberine suppressed sarcopenia insulin resistance through SIRT1-mediated mitophagy
Abnormal mitochondrial function resulting in inadequate energy supply leads to sarcopenia and IR, suggesting that maintaining mitochondrial homeostasis by regulating mitophagy may be a promising strategy for sarcopenia IR therapy. Herein, we constructed sarcopenia mice model, which was treated with...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2023-07-01
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| Series: | Open Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/biol-2022-0648 |
| _version_ | 1827894443354619904 |
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| author | Su Xiaojuan Yang Danqi Hu Yu Yuan Ying Song Le |
| author_facet | Su Xiaojuan Yang Danqi Hu Yu Yuan Ying Song Le |
| author_sort | Su Xiaojuan |
| collection | DOAJ |
| description | Abnormal mitochondrial function resulting in inadequate energy supply leads to sarcopenia and IR, suggesting that maintaining mitochondrial homeostasis by regulating mitophagy may be a promising strategy for sarcopenia IR therapy. Herein, we constructed sarcopenia mice model, which was treated with berberine and/or SIRT1/mitophagy inhibitors, and the activity of SIRT1/mitophagy signaling pathway was identified. Then, muscle tissue, blood biochemical index, inflammatory factors, GTT, and ITT were detected. We found that berberine treatment increased the body weight and alleviated d-galactose-induced weight loss in mice. SIRT1/mitophagy inhibitors suppressed the effects of berberine in the treatment of sarcopenia. The effect of berberine on the increase of muscle tissue, improving metabolic disorders, reducing the expression of inflammatory factors, and suppressing sarcopenia insulin resistance (IR) were reversed by SIRT1/mitophagy inhibitors. Our study establishes proof-of-concept to distinct the effect of berberine in sarcopenia IR, and provides strong evidence to support the hypothesis that berberine-induced SIRT1 triggers mitochondrial autophagy pathway and suppresses IR in sarcopenia. |
| first_indexed | 2024-03-12T22:06:41Z |
| format | Article |
| id | doaj.art-00a9bdee95244b689a69fa96cf59ed01 |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-03-12T22:06:41Z |
| publishDate | 2023-07-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-00a9bdee95244b689a69fa96cf59ed012023-07-24T11:18:21ZengDe GruyterOpen Life Sciences2391-54122023-07-011818539210.1515/biol-2022-0648Berberine suppressed sarcopenia insulin resistance through SIRT1-mediated mitophagySu Xiaojuan0Yang Danqi1Hu Yu2Yuan Ying3Song Le4Department of Geriatric, Zhongshan Hospital (Xiamen), Fudan University, Xiamen 361015, ChinaDepartment of Geriatric, Zhongshan Hospital (Xiamen), Fudan University, Xiamen 361015, ChinaDepartment of Geriatric, Zhongshan Hospital, Fudan University, No. 180, Fenglin Road, Xuhui District, Shanghai 200032, ChinaDepartment of Geriatric, Zhongshan Hospital, Fudan University, No. 180, Fenglin Road, Xuhui District, Shanghai 200032, ChinaDepartment of Geriatric, Zhongshan Hospital, Fudan University, No. 180, Fenglin Road, Xuhui District, Shanghai 200032, ChinaAbnormal mitochondrial function resulting in inadequate energy supply leads to sarcopenia and IR, suggesting that maintaining mitochondrial homeostasis by regulating mitophagy may be a promising strategy for sarcopenia IR therapy. Herein, we constructed sarcopenia mice model, which was treated with berberine and/or SIRT1/mitophagy inhibitors, and the activity of SIRT1/mitophagy signaling pathway was identified. Then, muscle tissue, blood biochemical index, inflammatory factors, GTT, and ITT were detected. We found that berberine treatment increased the body weight and alleviated d-galactose-induced weight loss in mice. SIRT1/mitophagy inhibitors suppressed the effects of berberine in the treatment of sarcopenia. The effect of berberine on the increase of muscle tissue, improving metabolic disorders, reducing the expression of inflammatory factors, and suppressing sarcopenia insulin resistance (IR) were reversed by SIRT1/mitophagy inhibitors. Our study establishes proof-of-concept to distinct the effect of berberine in sarcopenia IR, and provides strong evidence to support the hypothesis that berberine-induced SIRT1 triggers mitochondrial autophagy pathway and suppresses IR in sarcopenia.https://doi.org/10.1515/biol-2022-0648berberinesarcopeniainsulin resistancesirt1-mediated mitophagy |
| spellingShingle | Su Xiaojuan Yang Danqi Hu Yu Yuan Ying Song Le Berberine suppressed sarcopenia insulin resistance through SIRT1-mediated mitophagy Open Life Sciences berberine sarcopenia insulin resistance sirt1-mediated mitophagy |
| title | Berberine suppressed sarcopenia insulin resistance through SIRT1-mediated mitophagy |
| title_full | Berberine suppressed sarcopenia insulin resistance through SIRT1-mediated mitophagy |
| title_fullStr | Berberine suppressed sarcopenia insulin resistance through SIRT1-mediated mitophagy |
| title_full_unstemmed | Berberine suppressed sarcopenia insulin resistance through SIRT1-mediated mitophagy |
| title_short | Berberine suppressed sarcopenia insulin resistance through SIRT1-mediated mitophagy |
| title_sort | berberine suppressed sarcopenia insulin resistance through sirt1 mediated mitophagy |
| topic | berberine sarcopenia insulin resistance sirt1-mediated mitophagy |
| url | https://doi.org/10.1515/biol-2022-0648 |
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