Neutrophil extracellular traps in CSF and serum of dogs with steroid-responsive meningitis-arteritis.
In steroid-responsive meningitis-arteritis (SRMA), inflammatory dysregulation is driven by neutrophilic granulocytes resulting in purulent leptomeningitis. Neutrophils can generate neutrophil extracellular traps (NET). Uncontrolled NET-formation or impaired NET-clearance evidently cause tissue and o...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0295268&type=printable |
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| author | Jan Christian Wohlsein Marita Meurer Matthias Mörgelin Jasmin Nicole Nessler Thomas Flegel Henning Christian Schenk Konrad Jurina Kai Rentmeister Andrea Fischer Thomas Gödde Wolfgang Baumgärtner Maren von Köckritz-Blickwede Andrea Tipold |
| author_facet | Jan Christian Wohlsein Marita Meurer Matthias Mörgelin Jasmin Nicole Nessler Thomas Flegel Henning Christian Schenk Konrad Jurina Kai Rentmeister Andrea Fischer Thomas Gödde Wolfgang Baumgärtner Maren von Köckritz-Blickwede Andrea Tipold |
| author_sort | Jan Christian Wohlsein |
| collection | DOAJ |
| description | In steroid-responsive meningitis-arteritis (SRMA), inflammatory dysregulation is driven by neutrophilic granulocytes resulting in purulent leptomeningitis. Neutrophils can generate neutrophil extracellular traps (NET). Uncontrolled NET-formation or impaired NET-clearance evidently cause tissue and organ damage resulting in immune-mediated diseases. The aim of the study was to verify that NET-formation is detectable in ex vivo samples of acute diseased dogs with SRMA by visualizing and measuring NET-markers in serum and cerebrospinal fluid (CSF) samples. CSF-samples of dogs with acute SRMA (n = 5) and in remission (n = 4) were examined using immunofluorescence (IF)-staining of DNA-histone-1-complexes, myeloperoxidase and citrullinated Histone H3 (H3Cit). Immunogold-labeling of H3Cit and neutrophil elastase followed by transmission electron microscopy (TEM) were used to determine ultrastructural NET-formation in the CSF of one exemplary dog. H3Cit-levels and DNase-activity were measured in CSF and serum samples using an H3Cit-ELISA and a DNase-activity-assay, respectively in patients with the following diseases: acute SRMA (n = 34), SRMA in remission (n = 4), bacterial encephalitis (n = 3), meningioma with neutrophilic inflammation (n = 4), healthy dogs (n = 6). NET-formation was detectable with IF-staining in n = 3/5 CSF samples of dogs with acute SRMA but were not detectable during remission. Vesicular NET-formation was detectable in one exemplary dog using TEM. DNase-activity was significantly reduced in dogs suffering from acute SRMA compared to healthy control group (p < 0.0001). There were no statistical differences of H3Cit levels in CSF or serum samples of acute diseased dogs compared to dogs under treatment, dogs suffering from meningioma or bacterial encephalitis or the healthy control group. Our findings demonstrate that NET-formation and insufficient NET-clearance possibly drive the immunologic dysregulation and complement the pathogenesis of SRMA. The detection of NETs in SRMA offers many possibilities to explore the aetiopathogenetic influence of this defence mechanism of the innate immune system in infectious and non-infectious canine neuropathies. |
| first_indexed | 2024-03-08T12:28:50Z |
| format | Article |
| id | doaj.art-00a9f30994584508b02eedc637320873 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-08T12:28:50Z |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-00a9f30994584508b02eedc6373208732024-01-22T05:31:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01191e029526810.1371/journal.pone.0295268Neutrophil extracellular traps in CSF and serum of dogs with steroid-responsive meningitis-arteritis.Jan Christian WohlseinMarita MeurerMatthias MörgelinJasmin Nicole NesslerThomas FlegelHenning Christian SchenkKonrad JurinaKai RentmeisterAndrea FischerThomas GöddeWolfgang BaumgärtnerMaren von Köckritz-BlickwedeAndrea TipoldIn steroid-responsive meningitis-arteritis (SRMA), inflammatory dysregulation is driven by neutrophilic granulocytes resulting in purulent leptomeningitis. Neutrophils can generate neutrophil extracellular traps (NET). Uncontrolled NET-formation or impaired NET-clearance evidently cause tissue and organ damage resulting in immune-mediated diseases. The aim of the study was to verify that NET-formation is detectable in ex vivo samples of acute diseased dogs with SRMA by visualizing and measuring NET-markers in serum and cerebrospinal fluid (CSF) samples. CSF-samples of dogs with acute SRMA (n = 5) and in remission (n = 4) were examined using immunofluorescence (IF)-staining of DNA-histone-1-complexes, myeloperoxidase and citrullinated Histone H3 (H3Cit). Immunogold-labeling of H3Cit and neutrophil elastase followed by transmission electron microscopy (TEM) were used to determine ultrastructural NET-formation in the CSF of one exemplary dog. H3Cit-levels and DNase-activity were measured in CSF and serum samples using an H3Cit-ELISA and a DNase-activity-assay, respectively in patients with the following diseases: acute SRMA (n = 34), SRMA in remission (n = 4), bacterial encephalitis (n = 3), meningioma with neutrophilic inflammation (n = 4), healthy dogs (n = 6). NET-formation was detectable with IF-staining in n = 3/5 CSF samples of dogs with acute SRMA but were not detectable during remission. Vesicular NET-formation was detectable in one exemplary dog using TEM. DNase-activity was significantly reduced in dogs suffering from acute SRMA compared to healthy control group (p < 0.0001). There were no statistical differences of H3Cit levels in CSF or serum samples of acute diseased dogs compared to dogs under treatment, dogs suffering from meningioma or bacterial encephalitis or the healthy control group. Our findings demonstrate that NET-formation and insufficient NET-clearance possibly drive the immunologic dysregulation and complement the pathogenesis of SRMA. The detection of NETs in SRMA offers many possibilities to explore the aetiopathogenetic influence of this defence mechanism of the innate immune system in infectious and non-infectious canine neuropathies.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0295268&type=printable |
| spellingShingle | Jan Christian Wohlsein Marita Meurer Matthias Mörgelin Jasmin Nicole Nessler Thomas Flegel Henning Christian Schenk Konrad Jurina Kai Rentmeister Andrea Fischer Thomas Gödde Wolfgang Baumgärtner Maren von Köckritz-Blickwede Andrea Tipold Neutrophil extracellular traps in CSF and serum of dogs with steroid-responsive meningitis-arteritis. PLoS ONE |
| title | Neutrophil extracellular traps in CSF and serum of dogs with steroid-responsive meningitis-arteritis. |
| title_full | Neutrophil extracellular traps in CSF and serum of dogs with steroid-responsive meningitis-arteritis. |
| title_fullStr | Neutrophil extracellular traps in CSF and serum of dogs with steroid-responsive meningitis-arteritis. |
| title_full_unstemmed | Neutrophil extracellular traps in CSF and serum of dogs with steroid-responsive meningitis-arteritis. |
| title_short | Neutrophil extracellular traps in CSF and serum of dogs with steroid-responsive meningitis-arteritis. |
| title_sort | neutrophil extracellular traps in csf and serum of dogs with steroid responsive meningitis arteritis |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0295268&type=printable |
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