Functional Analysis of the Asian Soybean Rust Resistance Pathway Mediated by Rpp2
Asian soybean rust is an aggressive foliar disease caused by the obligate biotrophic fungus Phakopsora pachyrhizi. On susceptible plants, the pathogen penetrates and colonizes leaf tissue, resulting in the formation of necrotic lesions and the development of numerous uredinia. The soybean Rpp2 gene...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2011-02-01
|
| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-10-0187 |
| _version_ | 1811277663966330880 |
|---|---|
| author | Ajay K. Pandey Chunling Yang Chunquan Zhang Michelle A. Graham Heidi D. Horstman Yeunsook Lee Olga A. Zabotina John H. Hill Kerry F. Pedley Steven A. Whitham |
| author_facet | Ajay K. Pandey Chunling Yang Chunquan Zhang Michelle A. Graham Heidi D. Horstman Yeunsook Lee Olga A. Zabotina John H. Hill Kerry F. Pedley Steven A. Whitham |
| author_sort | Ajay K. Pandey |
| collection | DOAJ |
| description | Asian soybean rust is an aggressive foliar disease caused by the obligate biotrophic fungus Phakopsora pachyrhizi. On susceptible plants, the pathogen penetrates and colonizes leaf tissue, resulting in the formation of necrotic lesions and the development of numerous uredinia. The soybean Rpp2 gene confers resistance to specific isolates of P. pachyrhizi. Rpp2-mediated resistance limits the growth of the pathogen and is characterized by the formation of reddish-brown lesions and few uredinia. Using virus-induced gene silencing, we screened 140 candidate genes to identify those that play a role in Rpp2 resistance toward P. pachyrhizi. Candidate genes included putative orthologs to known defense-signaling genes, transcription factors, and genes previously found to be upregulated during the Rpp2 resistance response. We identified 11 genes that compromised Rpp2-mediated resistance when silenced, including GmEDS1, GmNPR1, GmPAD4, GmPAL1, five predicted transcription factors, an O-methyl transferase, and a cytochrome P450 monooxygenase. Together, our results provide new insight into the signaling and biochemical pathways required for resistance against P. pachyrhizi. |
| first_indexed | 2024-04-13T00:20:49Z |
| format | Article |
| id | doaj.art-00aad761702a441fa6d62242d4578a0f |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T00:20:49Z |
| publishDate | 2011-02-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-00aad761702a441fa6d62242d4578a0f2022-12-22T03:10:45ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062011-02-0124219420610.1094/MPMI-08-10-0187Functional Analysis of the Asian Soybean Rust Resistance Pathway Mediated by Rpp2Ajay K. PandeyChunling YangChunquan ZhangMichelle A. GrahamHeidi D. HorstmanYeunsook LeeOlga A. ZabotinaJohn H. HillKerry F. PedleySteven A. WhithamAsian soybean rust is an aggressive foliar disease caused by the obligate biotrophic fungus Phakopsora pachyrhizi. On susceptible plants, the pathogen penetrates and colonizes leaf tissue, resulting in the formation of necrotic lesions and the development of numerous uredinia. The soybean Rpp2 gene confers resistance to specific isolates of P. pachyrhizi. Rpp2-mediated resistance limits the growth of the pathogen and is characterized by the formation of reddish-brown lesions and few uredinia. Using virus-induced gene silencing, we screened 140 candidate genes to identify those that play a role in Rpp2 resistance toward P. pachyrhizi. Candidate genes included putative orthologs to known defense-signaling genes, transcription factors, and genes previously found to be upregulated during the Rpp2 resistance response. We identified 11 genes that compromised Rpp2-mediated resistance when silenced, including GmEDS1, GmNPR1, GmPAD4, GmPAL1, five predicted transcription factors, an O-methyl transferase, and a cytochrome P450 monooxygenase. Together, our results provide new insight into the signaling and biochemical pathways required for resistance against P. pachyrhizi.https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-10-0187 |
| spellingShingle | Ajay K. Pandey Chunling Yang Chunquan Zhang Michelle A. Graham Heidi D. Horstman Yeunsook Lee Olga A. Zabotina John H. Hill Kerry F. Pedley Steven A. Whitham Functional Analysis of the Asian Soybean Rust Resistance Pathway Mediated by Rpp2 Molecular Plant-Microbe Interactions |
| title | Functional Analysis of the Asian Soybean Rust Resistance Pathway Mediated by Rpp2 |
| title_full | Functional Analysis of the Asian Soybean Rust Resistance Pathway Mediated by Rpp2 |
| title_fullStr | Functional Analysis of the Asian Soybean Rust Resistance Pathway Mediated by Rpp2 |
| title_full_unstemmed | Functional Analysis of the Asian Soybean Rust Resistance Pathway Mediated by Rpp2 |
| title_short | Functional Analysis of the Asian Soybean Rust Resistance Pathway Mediated by Rpp2 |
| title_sort | functional analysis of the asian soybean rust resistance pathway mediated by rpp2 |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-10-0187 |
| work_keys_str_mv | AT ajaykpandey functionalanalysisoftheasiansoybeanrustresistancepathwaymediatedbyrpp2 AT chunlingyang functionalanalysisoftheasiansoybeanrustresistancepathwaymediatedbyrpp2 AT chunquanzhang functionalanalysisoftheasiansoybeanrustresistancepathwaymediatedbyrpp2 AT michelleagraham functionalanalysisoftheasiansoybeanrustresistancepathwaymediatedbyrpp2 AT heididhorstman functionalanalysisoftheasiansoybeanrustresistancepathwaymediatedbyrpp2 AT yeunsooklee functionalanalysisoftheasiansoybeanrustresistancepathwaymediatedbyrpp2 AT olgaazabotina functionalanalysisoftheasiansoybeanrustresistancepathwaymediatedbyrpp2 AT johnhhill functionalanalysisoftheasiansoybeanrustresistancepathwaymediatedbyrpp2 AT kerryfpedley functionalanalysisoftheasiansoybeanrustresistancepathwaymediatedbyrpp2 AT stevenawhitham functionalanalysisoftheasiansoybeanrustresistancepathwaymediatedbyrpp2 |