The application research of xTAG GPP multiplex PCR in the diagnosis of persistent and chronic diarrhea in children
Abstract Background Persistent and chronic diarrhea is difficult to treat, and infection is still the main cause. In this study, we investigate the application value of xTAG gastrointestinal pathogen panel (xTAG GPP) multiplex PCR in the early diagnosis of persistent and chronic diarrhea in children...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-06-01
|
| Series: | BMC Pediatrics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12887-020-02206-6 |
| _version_ | 1819000854679650304 |
|---|---|
| author | Chunli Wang Xiaoying Zhou Mengshu Zhu Hanjun Yin Jiamei Tang Yan Huang Bixia Zheng Yu Jin Zhifeng Liu |
| author_facet | Chunli Wang Xiaoying Zhou Mengshu Zhu Hanjun Yin Jiamei Tang Yan Huang Bixia Zheng Yu Jin Zhifeng Liu |
| author_sort | Chunli Wang |
| collection | DOAJ |
| description | Abstract Background Persistent and chronic diarrhea is difficult to treat, and infection is still the main cause. In this study, we investigate the application value of xTAG gastrointestinal pathogen panel (xTAG GPP) multiplex PCR in the early diagnosis of persistent and chronic diarrhea in children and to understand the epidemiology of intestinal diarrhea pathogens. Methods One hundred ninety-nine specimens were collected from Nanjing Children’s Hospital Affiliated to Nanjing Medical University (Nanjing, China). We compared the xTAG GPP multiplex PCR assay with traditional methods (culture, rapid enzyme immunoassay chromatography, and microscopic examination) and performed a statistical analysis. Results The positive rate of the xTAG GPP multiplex PCR assay of diarrhea specimens from 199 patients was 72.86% (145/199). The virus detection rate was 48.7%, and rotavirus A was the most common organism detected (34.67%), concentrated in winter, and was common in children. The second most common organism detected was norovirus GI/GII (20.6%). The positive rate of this bacteria was 40.2%, and Campylobacter (22.11%, 44/199) was most frequently detected. C. difficile toxins A/B and Salmonella was detected in 44 and 17 samples, respectively. Infections with Shigella occurred 4 times, and E. coli O157 was only detected once. Three samples were parasitic (1.51%), two samples were positive for Entamoeba histolytica, and one was positive for Cryptosporidium. Adenovirus 40/41, STEC, ETEC, Giardia, Yersinia enterocolitica and Vibrio cholerae were not detected. In total, 86 (43.2%) infected specimens with a single pathogen were detected. There were 59 coinfections (29.65% of the samples) of viruses and/or bacteria and/or parasites. Coinfections involved 49 double infections (24.62%), 9 triple infections (4.52%) and 1 quadruple infections (0.5%). Norovirus GI/GII was found to have the highest involvement, with 32 coinfections (16.08%). Conclusion The xTAG GPP multiplex PCR assay is simple, sensitive, and specific and can be used as a quick way to diagnose persistent and chronic diarrhea in children. |
| first_indexed | 2024-12-20T22:39:56Z |
| format | Article |
| id | doaj.art-00b0d6eef16740f89ff4290b22d43f5a |
| institution | Directory Open Access Journal |
| issn | 1471-2431 |
| language | English |
| last_indexed | 2024-12-20T22:39:56Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pediatrics |
| spelling | doaj.art-00b0d6eef16740f89ff4290b22d43f5a2022-12-21T19:24:29ZengBMCBMC Pediatrics1471-24312020-06-012011710.1186/s12887-020-02206-6The application research of xTAG GPP multiplex PCR in the diagnosis of persistent and chronic diarrhea in childrenChunli Wang0Xiaoying Zhou1Mengshu Zhu2Hanjun Yin3Jiamei Tang4Yan Huang5Bixia Zheng6Yu Jin7Zhifeng Liu8Nanjing Key Laboratory of Pediatrics, Children’s Hospital of Nanjing Medical UniversityDepartment of Gastroenterology, Children’s Hospital of Nanjing Medical UniversityDepartment of Gastroenterology, Children’s Hospital of Nanjing Medical UniversityDepartment of Gastroenterology, Children’s Hospital of Nanjing Medical UniversityDepartment of Gastroenterology, Children’s Hospital of Nanjing Medical UniversityDepartment of Gastroenterology, Children’s Hospital of Nanjing Medical UniversityNanjing Key Laboratory of Pediatrics, Children’s Hospital of Nanjing Medical UniversityDepartment of Gastroenterology, Children’s Hospital of Nanjing Medical UniversityDepartment of Gastroenterology, Children’s Hospital of Nanjing Medical UniversityAbstract Background Persistent and chronic diarrhea is difficult to treat, and infection is still the main cause. In this study, we investigate the application value of xTAG gastrointestinal pathogen panel (xTAG GPP) multiplex PCR in the early diagnosis of persistent and chronic diarrhea in children and to understand the epidemiology of intestinal diarrhea pathogens. Methods One hundred ninety-nine specimens were collected from Nanjing Children’s Hospital Affiliated to Nanjing Medical University (Nanjing, China). We compared the xTAG GPP multiplex PCR assay with traditional methods (culture, rapid enzyme immunoassay chromatography, and microscopic examination) and performed a statistical analysis. Results The positive rate of the xTAG GPP multiplex PCR assay of diarrhea specimens from 199 patients was 72.86% (145/199). The virus detection rate was 48.7%, and rotavirus A was the most common organism detected (34.67%), concentrated in winter, and was common in children. The second most common organism detected was norovirus GI/GII (20.6%). The positive rate of this bacteria was 40.2%, and Campylobacter (22.11%, 44/199) was most frequently detected. C. difficile toxins A/B and Salmonella was detected in 44 and 17 samples, respectively. Infections with Shigella occurred 4 times, and E. coli O157 was only detected once. Three samples were parasitic (1.51%), two samples were positive for Entamoeba histolytica, and one was positive for Cryptosporidium. Adenovirus 40/41, STEC, ETEC, Giardia, Yersinia enterocolitica and Vibrio cholerae were not detected. In total, 86 (43.2%) infected specimens with a single pathogen were detected. There were 59 coinfections (29.65% of the samples) of viruses and/or bacteria and/or parasites. Coinfections involved 49 double infections (24.62%), 9 triple infections (4.52%) and 1 quadruple infections (0.5%). Norovirus GI/GII was found to have the highest involvement, with 32 coinfections (16.08%). Conclusion The xTAG GPP multiplex PCR assay is simple, sensitive, and specific and can be used as a quick way to diagnose persistent and chronic diarrhea in children.http://link.springer.com/article/10.1186/s12887-020-02206-6Persistent diarrheaChronic diarrheaNucleic acid amplification techniquesVirusesBacteriaParasites |
| spellingShingle | Chunli Wang Xiaoying Zhou Mengshu Zhu Hanjun Yin Jiamei Tang Yan Huang Bixia Zheng Yu Jin Zhifeng Liu The application research of xTAG GPP multiplex PCR in the diagnosis of persistent and chronic diarrhea in children BMC Pediatrics Persistent diarrhea Chronic diarrhea Nucleic acid amplification techniques Viruses Bacteria Parasites |
| title | The application research of xTAG GPP multiplex PCR in the diagnosis of persistent and chronic diarrhea in children |
| title_full | The application research of xTAG GPP multiplex PCR in the diagnosis of persistent and chronic diarrhea in children |
| title_fullStr | The application research of xTAG GPP multiplex PCR in the diagnosis of persistent and chronic diarrhea in children |
| title_full_unstemmed | The application research of xTAG GPP multiplex PCR in the diagnosis of persistent and chronic diarrhea in children |
| title_short | The application research of xTAG GPP multiplex PCR in the diagnosis of persistent and chronic diarrhea in children |
| title_sort | application research of xtag gpp multiplex pcr in the diagnosis of persistent and chronic diarrhea in children |
| topic | Persistent diarrhea Chronic diarrhea Nucleic acid amplification techniques Viruses Bacteria Parasites |
| url | http://link.springer.com/article/10.1186/s12887-020-02206-6 |
| work_keys_str_mv | AT chunliwang theapplicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren AT xiaoyingzhou theapplicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren AT mengshuzhu theapplicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren AT hanjunyin theapplicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren AT jiameitang theapplicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren AT yanhuang theapplicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren AT bixiazheng theapplicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren AT yujin theapplicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren AT zhifengliu theapplicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren AT chunliwang applicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren AT xiaoyingzhou applicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren AT mengshuzhu applicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren AT hanjunyin applicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren AT jiameitang applicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren AT yanhuang applicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren AT bixiazheng applicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren AT yujin applicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren AT zhifengliu applicationresearchofxtaggppmultiplexpcrinthediagnosisofpersistentandchronicdiarrheainchildren |