Characterization of the Phenanthrene-Degrading <i>Sphingobium yanoikuyae</i> SJTF8 in Heavy Metal Co-Existing Liquid Medium and Analysis of Its Metabolic Pathway
Polycyclic aromatic hydrocarbons (PAHs) are common organic pollutants with great carcinogenic threaten, and metal/PAH-contaminated environments represent one of the most difficult remedial challenges. In this work, <i>Sphingobium yanoikuyae</i> SJTF8 was isolated and identified with grea...
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MDPI AG
2020-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/6/946 |
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| author | Chong Yin Weiliang Xiong Hua Qiu Wanli Peng Zixin Deng Shuangjun Lin Rubing Liang |
| author_facet | Chong Yin Weiliang Xiong Hua Qiu Wanli Peng Zixin Deng Shuangjun Lin Rubing Liang |
| author_sort | Chong Yin |
| collection | DOAJ |
| description | Polycyclic aromatic hydrocarbons (PAHs) are common organic pollutants with great carcinogenic threaten, and metal/PAH-contaminated environments represent one of the most difficult remedial challenges. In this work, <i>Sphingobium yanoikuyae</i> SJTF8 was isolated and identified with great and stable PAH-degrading efficiency even under stress conditions. It could utilize typical PAHs (naphthalene, phenanthrene, and anthracene) and heterocyclic and halogenated aromatic compounds (dibenzothiophene and 9-bromophenanthrene) as the sole carbon source. It could degrade over 98% of 500 mg/L phenanthrene in 4 days, and the <i>cis</i>-3,4-dihydrophenanthrene-3,4-diol was the first-step intermediate. Notably, strain SJTF8 showed great tolerance to heavy metals and acidic pH. Supplements of 0.30 mM of Cu<sup>2+</sup>, 1.15 mM of Zn<sup>2+</sup>, and 0.01 mM of Cd<sup>2+</sup> had little effect on its cell growth and phenanthrene degradation; phenanthrene of 250 mg/L could still be degraded completely in 48 h. Further, the whole genome sequence of <i>S. yanoikuyae</i> SJTF8 was obtained, and three plasmids were found. The potential genes participating in stress-tolerance and PAH-degradation were annotated and were found mostly distributed in plasmids 1 and 2. Elimination of plasmid 2 resulted in the loss of the PAH-degradation ability. On the basis of genome mining results, the possible degrading pathway and the metabolites of <i>S. yanoikuyae</i> SJTF8 to phenanthrene were predicted. |
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| publishDate | 2020-06-01 |
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| spelling | doaj.art-00b205063a73462d812ea1a385e09b492023-11-20T04:45:01ZengMDPI AGMicroorganisms2076-26072020-06-018694610.3390/microorganisms8060946Characterization of the Phenanthrene-Degrading <i>Sphingobium yanoikuyae</i> SJTF8 in Heavy Metal Co-Existing Liquid Medium and Analysis of Its Metabolic PathwayChong Yin0Weiliang Xiong1Hua Qiu2Wanli Peng3Zixin Deng4Shuangjun Lin5Rubing Liang6State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaPolycyclic aromatic hydrocarbons (PAHs) are common organic pollutants with great carcinogenic threaten, and metal/PAH-contaminated environments represent one of the most difficult remedial challenges. In this work, <i>Sphingobium yanoikuyae</i> SJTF8 was isolated and identified with great and stable PAH-degrading efficiency even under stress conditions. It could utilize typical PAHs (naphthalene, phenanthrene, and anthracene) and heterocyclic and halogenated aromatic compounds (dibenzothiophene and 9-bromophenanthrene) as the sole carbon source. It could degrade over 98% of 500 mg/L phenanthrene in 4 days, and the <i>cis</i>-3,4-dihydrophenanthrene-3,4-diol was the first-step intermediate. Notably, strain SJTF8 showed great tolerance to heavy metals and acidic pH. Supplements of 0.30 mM of Cu<sup>2+</sup>, 1.15 mM of Zn<sup>2+</sup>, and 0.01 mM of Cd<sup>2+</sup> had little effect on its cell growth and phenanthrene degradation; phenanthrene of 250 mg/L could still be degraded completely in 48 h. Further, the whole genome sequence of <i>S. yanoikuyae</i> SJTF8 was obtained, and three plasmids were found. The potential genes participating in stress-tolerance and PAH-degradation were annotated and were found mostly distributed in plasmids 1 and 2. Elimination of plasmid 2 resulted in the loss of the PAH-degradation ability. On the basis of genome mining results, the possible degrading pathway and the metabolites of <i>S. yanoikuyae</i> SJTF8 to phenanthrene were predicted.https://www.mdpi.com/2076-2607/8/6/946polycyclic aromatic hydrocarbons<i>Sphingobium yanoikuyae</i> SJTF8phenanthreneheavy metalbioremediationtolerance |
| spellingShingle | Chong Yin Weiliang Xiong Hua Qiu Wanli Peng Zixin Deng Shuangjun Lin Rubing Liang Characterization of the Phenanthrene-Degrading <i>Sphingobium yanoikuyae</i> SJTF8 in Heavy Metal Co-Existing Liquid Medium and Analysis of Its Metabolic Pathway Microorganisms polycyclic aromatic hydrocarbons <i>Sphingobium yanoikuyae</i> SJTF8 phenanthrene heavy metal bioremediation tolerance |
| title | Characterization of the Phenanthrene-Degrading <i>Sphingobium yanoikuyae</i> SJTF8 in Heavy Metal Co-Existing Liquid Medium and Analysis of Its Metabolic Pathway |
| title_full | Characterization of the Phenanthrene-Degrading <i>Sphingobium yanoikuyae</i> SJTF8 in Heavy Metal Co-Existing Liquid Medium and Analysis of Its Metabolic Pathway |
| title_fullStr | Characterization of the Phenanthrene-Degrading <i>Sphingobium yanoikuyae</i> SJTF8 in Heavy Metal Co-Existing Liquid Medium and Analysis of Its Metabolic Pathway |
| title_full_unstemmed | Characterization of the Phenanthrene-Degrading <i>Sphingobium yanoikuyae</i> SJTF8 in Heavy Metal Co-Existing Liquid Medium and Analysis of Its Metabolic Pathway |
| title_short | Characterization of the Phenanthrene-Degrading <i>Sphingobium yanoikuyae</i> SJTF8 in Heavy Metal Co-Existing Liquid Medium and Analysis of Its Metabolic Pathway |
| title_sort | characterization of the phenanthrene degrading i sphingobium yanoikuyae i sjtf8 in heavy metal co existing liquid medium and analysis of its metabolic pathway |
| topic | polycyclic aromatic hydrocarbons <i>Sphingobium yanoikuyae</i> SJTF8 phenanthrene heavy metal bioremediation tolerance |
| url | https://www.mdpi.com/2076-2607/8/6/946 |
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