Defining the Skeletal Myogenic Lineage in Human Pluripotent Stem Cell-Derived Teratomas
Skeletal muscle stem cells are essential to muscle homeostasis and regeneration after injury, and have emerged as a promising cell source for treating skeletal disorders. An attractive approach to obtain these cells utilizes differentiation of pluripotent stem cells (PSCs). We recently reported that...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/9/1589 |
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| author | Matthew P. Pappas Ning Xie Jacqueline S. Penaloza Sunny S. K. Chan |
| author_facet | Matthew P. Pappas Ning Xie Jacqueline S. Penaloza Sunny S. K. Chan |
| author_sort | Matthew P. Pappas |
| collection | DOAJ |
| description | Skeletal muscle stem cells are essential to muscle homeostasis and regeneration after injury, and have emerged as a promising cell source for treating skeletal disorders. An attractive approach to obtain these cells utilizes differentiation of pluripotent stem cells (PSCs). We recently reported that teratomas derived from mouse PSCs are a rich source of skeletal muscle stem cells. Here, we showed that teratoma formation is also capable of producing skeletal myogenic progenitors from human PSCs. Using single-cell transcriptomics, we discovered several distinct skeletal myogenic subpopulations that represent progressive developmental stages of the skeletal myogenic lineage and recapitulate human embryonic skeletal myogenesis. We further discovered that ERBB3 and CD82 are effective surface markers for prospective isolation of the skeletal myogenic lineage in human PSC-derived teratomas. Therefore, teratoma formation provides an accessible model for obtaining human skeletal myogenic progenitors from PSCs. |
| first_indexed | 2024-03-10T04:15:36Z |
| format | Article |
| id | doaj.art-00b2298c258146848e8511be9f02eb65 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T04:15:36Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-00b2298c258146848e8511be9f02eb652023-11-23T08:01:27ZengMDPI AGCells2073-44092022-05-01119158910.3390/cells11091589Defining the Skeletal Myogenic Lineage in Human Pluripotent Stem Cell-Derived TeratomasMatthew P. Pappas0Ning Xie1Jacqueline S. Penaloza2Sunny S. K. Chan3Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USADepartment of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USADepartment of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USADepartment of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USASkeletal muscle stem cells are essential to muscle homeostasis and regeneration after injury, and have emerged as a promising cell source for treating skeletal disorders. An attractive approach to obtain these cells utilizes differentiation of pluripotent stem cells (PSCs). We recently reported that teratomas derived from mouse PSCs are a rich source of skeletal muscle stem cells. Here, we showed that teratoma formation is also capable of producing skeletal myogenic progenitors from human PSCs. Using single-cell transcriptomics, we discovered several distinct skeletal myogenic subpopulations that represent progressive developmental stages of the skeletal myogenic lineage and recapitulate human embryonic skeletal myogenesis. We further discovered that ERBB3 and CD82 are effective surface markers for prospective isolation of the skeletal myogenic lineage in human PSC-derived teratomas. Therefore, teratoma formation provides an accessible model for obtaining human skeletal myogenic progenitors from PSCs.https://www.mdpi.com/2073-4409/11/9/1589pluripotent stem cellsmyogenic developmentmuscle stem cellssatellite cells |
| spellingShingle | Matthew P. Pappas Ning Xie Jacqueline S. Penaloza Sunny S. K. Chan Defining the Skeletal Myogenic Lineage in Human Pluripotent Stem Cell-Derived Teratomas Cells pluripotent stem cells myogenic development muscle stem cells satellite cells |
| title | Defining the Skeletal Myogenic Lineage in Human Pluripotent Stem Cell-Derived Teratomas |
| title_full | Defining the Skeletal Myogenic Lineage in Human Pluripotent Stem Cell-Derived Teratomas |
| title_fullStr | Defining the Skeletal Myogenic Lineage in Human Pluripotent Stem Cell-Derived Teratomas |
| title_full_unstemmed | Defining the Skeletal Myogenic Lineage in Human Pluripotent Stem Cell-Derived Teratomas |
| title_short | Defining the Skeletal Myogenic Lineage in Human Pluripotent Stem Cell-Derived Teratomas |
| title_sort | defining the skeletal myogenic lineage in human pluripotent stem cell derived teratomas |
| topic | pluripotent stem cells myogenic development muscle stem cells satellite cells |
| url | https://www.mdpi.com/2073-4409/11/9/1589 |
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