Antibiofilm activity of Streptomyces toxytricini Fz94 against Candida albicans ATCC 10231
Candida albicans is a significant cause of morbidity and mortality in immunocompromised patients worldwide. Biofilm formation by Candida species is a significant virulence factor for disease pathogenesis. Keeping in view the importance of Streptomyces' metabolites, the present study was initiat...
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| Format: | Article |
| Language: | English |
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Arab Society for Fungal Conservation
2017-06-01
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| Series: | Microbial Biosystems Journal |
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| Online Access: | http://fungiofegypt.com/Journal/MBJ_2_1_4_Sheir-and-Hafez.pdf |
| _version_ | 1818639893842100224 |
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| author | Sheir DH Hafez MA |
| author_facet | Sheir DH Hafez MA |
| author_sort | Sheir DH |
| collection | DOAJ |
| description | Candida albicans is a significant cause of morbidity and mortality in immunocompromised patients worldwide. Biofilm formation by Candida species is a significant virulence factor for disease pathogenesis. Keeping in view the importance of Streptomyces' metabolites, the present study was initiated during the bioprospecting programme of Egyptian Streptomyces carried by the authors since 2013. Native Streptomyces isolates were recovered from soil samples collected from different governorates. Antifungal activity of forty isolates of Streptomyces were performed against planktonic (free cells) of C. albicans ATCC 10231 and resistant clinical Candida isolates. Streptomyces isolates showed high inhibition activity against free cells of Candida were further assayed against biofilm of C. albicans reference strain. The most active Streptomyces sp. (no.6) was identified phenotypically, biochemically and by using 16S rRNA. The 16S rRNA sequences obtained were compared with those deposited in the GenBank Database and registered with accession number KM052378 as S. toxytricini Fz94. Screening of S. toxytricini Fz94 extract capability in prevention and destruction of C. albicans reference strain biolfilm was assessed by resazurin dye adopted technique. In the pre-exposure scheme, the lowest concentration of 5 gL-1 showed biofilm viability inhibition of 92% after 120 min, while Ketoconazole® gave 90 % inhibition at concentration of 2 gL-1. In post exposure, the concentration of S. toxytricini Fz94 extract 7gL-1 caused 82 % inhibition of biofilms viability after 120 min, while Ketoconazole did not show any destruction capability. The cytotoxicity of S. toxytricini Fz94 crude extract results showed that it was nontoxic at 10 gL-1. S. toxytricini Fz94 is maintained in the Fungarium of Arab Society for Fungal Conservation (ASFC) with accession number FSCU-2017-1110. |
| first_indexed | 2024-12-16T23:02:37Z |
| format | Article |
| id | doaj.art-00b262d01a304273ab0a1ab2e2fffa88 |
| institution | Directory Open Access Journal |
| issn | 2357-0326 2357-0334 |
| language | English |
| last_indexed | 2024-12-16T23:02:37Z |
| publishDate | 2017-06-01 |
| publisher | Arab Society for Fungal Conservation |
| record_format | Article |
| series | Microbial Biosystems Journal |
| spelling | doaj.art-00b262d01a304273ab0a1ab2e2fffa882022-12-21T22:12:39ZengArab Society for Fungal ConservationMicrobial Biosystems Journal2357-03262357-03342017-06-01122639Antibiofilm activity of Streptomyces toxytricini Fz94 against Candida albicans ATCC 10231Sheir DH0Hafez MA1Chemistry of Natural and Microbial Products Department, Pharmaceutical Industries Division, National Research Centre, Cairo, 12622, Egypt.Department of Botany and Microbiology, Faculty of Science, Al Azhar University, Cairo, 11651, Egypt.Candida albicans is a significant cause of morbidity and mortality in immunocompromised patients worldwide. Biofilm formation by Candida species is a significant virulence factor for disease pathogenesis. Keeping in view the importance of Streptomyces' metabolites, the present study was initiated during the bioprospecting programme of Egyptian Streptomyces carried by the authors since 2013. Native Streptomyces isolates were recovered from soil samples collected from different governorates. Antifungal activity of forty isolates of Streptomyces were performed against planktonic (free cells) of C. albicans ATCC 10231 and resistant clinical Candida isolates. Streptomyces isolates showed high inhibition activity against free cells of Candida were further assayed against biofilm of C. albicans reference strain. The most active Streptomyces sp. (no.6) was identified phenotypically, biochemically and by using 16S rRNA. The 16S rRNA sequences obtained were compared with those deposited in the GenBank Database and registered with accession number KM052378 as S. toxytricini Fz94. Screening of S. toxytricini Fz94 extract capability in prevention and destruction of C. albicans reference strain biolfilm was assessed by resazurin dye adopted technique. In the pre-exposure scheme, the lowest concentration of 5 gL-1 showed biofilm viability inhibition of 92% after 120 min, while Ketoconazole® gave 90 % inhibition at concentration of 2 gL-1. In post exposure, the concentration of S. toxytricini Fz94 extract 7gL-1 caused 82 % inhibition of biofilms viability after 120 min, while Ketoconazole did not show any destruction capability. The cytotoxicity of S. toxytricini Fz94 crude extract results showed that it was nontoxic at 10 gL-1. S. toxytricini Fz94 is maintained in the Fungarium of Arab Society for Fungal Conservation (ASFC) with accession number FSCU-2017-1110.http://fungiofegypt.com/Journal/MBJ_2_1_4_Sheir-and-Hafez.pdfBioprospectingEgyptKetoconazoleStreptomyces toxytricini Fz94resazurin |
| spellingShingle | Sheir DH Hafez MA Antibiofilm activity of Streptomyces toxytricini Fz94 against Candida albicans ATCC 10231 Microbial Biosystems Journal Bioprospecting Egypt Ketoconazole Streptomyces toxytricini Fz94 resazurin |
| title | Antibiofilm activity of Streptomyces toxytricini Fz94 against Candida albicans ATCC 10231 |
| title_full | Antibiofilm activity of Streptomyces toxytricini Fz94 against Candida albicans ATCC 10231 |
| title_fullStr | Antibiofilm activity of Streptomyces toxytricini Fz94 against Candida albicans ATCC 10231 |
| title_full_unstemmed | Antibiofilm activity of Streptomyces toxytricini Fz94 against Candida albicans ATCC 10231 |
| title_short | Antibiofilm activity of Streptomyces toxytricini Fz94 against Candida albicans ATCC 10231 |
| title_sort | antibiofilm activity of streptomyces toxytricini fz94 against candida albicans atcc 10231 |
| topic | Bioprospecting Egypt Ketoconazole Streptomyces toxytricini Fz94 resazurin |
| url | http://fungiofegypt.com/Journal/MBJ_2_1_4_Sheir-and-Hafez.pdf |
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