Comparative Study on the Structural Properties and Bioactivities of Three Different Molecular Weights of <i>Lycium barbarum</i> Polysaccharides
The molecular weight, the triple-helix conformation, the monosaccharide content, the manner of glycosidic linkages, and the polysaccharide conjugates of polysaccharides all affect bioactivity. The purpose of this study was to determine how different molecular weights affected the bioactivity of the...
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MDPI AG
2023-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/2/701 |
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| author | Wenjun Zeng Lulu Chen Zhihui Xiao Yanping Li Jianlong Ma Jianbao Ding Jin Yang |
| author_facet | Wenjun Zeng Lulu Chen Zhihui Xiao Yanping Li Jianlong Ma Jianbao Ding Jin Yang |
| author_sort | Wenjun Zeng |
| collection | DOAJ |
| description | The molecular weight, the triple-helix conformation, the monosaccharide content, the manner of glycosidic linkages, and the polysaccharide conjugates of polysaccharides all affect bioactivity. The purpose of this study was to determine how different molecular weights affected the bioactivity of the <i>Lycium barbarum</i> polysaccharides (LBPs). By ethanol-graded precipitation and ultrafiltration membrane separation, one oligosaccharide (LBPs-1, 1.912 kDa) and two polysaccharides (LBPs-2, 7.481 kDa; LBPs-3, 46.239 kDa) were obtained from <i>Lycium barbarum</i>. While the major component of LBPs-1 and LBPs-2 was glucose, the main constituents of LBPs-3 were arabinose, galactose, and glucose. LBPs-2 and LBPs-3 exhibited triple-helix conformations, as evidenced by the Congo red experiment and AFM data. Sugar residues of LBPs-2 and LBPs-3 were elucidated by NMR spectra. The polysaccharides (LBPs-2 and LBPs-3) exhibited much higher antioxidant capacities than oligosaccharide (LBPs-1). LBPs-3 showed higher oxygen radical absorbance capacity (ORAC) and superoxide dismutase (SOD) activity than LBPs-2, but a lower capability for scavenging ABTS<sup>+</sup> radicals. In zebrafish, LBPs-2 and LBPs-3 boosted the growth of T-lymphocytes and macrophages, enhanced the immunological response, and mitigated the immune damage generated by VTI. In addition to the molecular weight, the results indicated that the biological activities would be the consequence of various aspects, such as the monosaccharide composition ratio, the chemical composition, and the chemical reaction mechanism. |
| first_indexed | 2024-03-09T11:34:08Z |
| format | Article |
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| language | English |
| last_indexed | 2024-03-09T11:34:08Z |
| publishDate | 2023-01-01 |
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| series | Molecules |
| spelling | doaj.art-00b3e91be2754fb2af9be9fff9266a7b2023-11-30T23:43:33ZengMDPI AGMolecules1420-30492023-01-0128270110.3390/molecules28020701Comparative Study on the Structural Properties and Bioactivities of Three Different Molecular Weights of <i>Lycium barbarum</i> PolysaccharidesWenjun Zeng0Lulu Chen1Zhihui Xiao2Yanping Li3Jianlong Ma4Jianbao Ding5Jin Yang6School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, ChinaSchool of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, ChinaSouth China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaNingxia Wuxing Science and Technology Co., Ltd., Yinchuan 750021, ChinaNingxia Research Center for Natural Medicine Engineering and Technology, Yinchuan 750021, ChinaNingxia Wuxing Science and Technology Co., Ltd., Yinchuan 750021, ChinaSchool of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, ChinaThe molecular weight, the triple-helix conformation, the monosaccharide content, the manner of glycosidic linkages, and the polysaccharide conjugates of polysaccharides all affect bioactivity. The purpose of this study was to determine how different molecular weights affected the bioactivity of the <i>Lycium barbarum</i> polysaccharides (LBPs). By ethanol-graded precipitation and ultrafiltration membrane separation, one oligosaccharide (LBPs-1, 1.912 kDa) and two polysaccharides (LBPs-2, 7.481 kDa; LBPs-3, 46.239 kDa) were obtained from <i>Lycium barbarum</i>. While the major component of LBPs-1 and LBPs-2 was glucose, the main constituents of LBPs-3 were arabinose, galactose, and glucose. LBPs-2 and LBPs-3 exhibited triple-helix conformations, as evidenced by the Congo red experiment and AFM data. Sugar residues of LBPs-2 and LBPs-3 were elucidated by NMR spectra. The polysaccharides (LBPs-2 and LBPs-3) exhibited much higher antioxidant capacities than oligosaccharide (LBPs-1). LBPs-3 showed higher oxygen radical absorbance capacity (ORAC) and superoxide dismutase (SOD) activity than LBPs-2, but a lower capability for scavenging ABTS<sup>+</sup> radicals. In zebrafish, LBPs-2 and LBPs-3 boosted the growth of T-lymphocytes and macrophages, enhanced the immunological response, and mitigated the immune damage generated by VTI. In addition to the molecular weight, the results indicated that the biological activities would be the consequence of various aspects, such as the monosaccharide composition ratio, the chemical composition, and the chemical reaction mechanism.https://www.mdpi.com/1420-3049/28/2/701<i>Lycium barbarum</i> polysaccharidesmolecular weightchemical characteristicsantioxidant activityimmunomodulatory activity |
| spellingShingle | Wenjun Zeng Lulu Chen Zhihui Xiao Yanping Li Jianlong Ma Jianbao Ding Jin Yang Comparative Study on the Structural Properties and Bioactivities of Three Different Molecular Weights of <i>Lycium barbarum</i> Polysaccharides Molecules <i>Lycium barbarum</i> polysaccharides molecular weight chemical characteristics antioxidant activity immunomodulatory activity |
| title | Comparative Study on the Structural Properties and Bioactivities of Three Different Molecular Weights of <i>Lycium barbarum</i> Polysaccharides |
| title_full | Comparative Study on the Structural Properties and Bioactivities of Three Different Molecular Weights of <i>Lycium barbarum</i> Polysaccharides |
| title_fullStr | Comparative Study on the Structural Properties and Bioactivities of Three Different Molecular Weights of <i>Lycium barbarum</i> Polysaccharides |
| title_full_unstemmed | Comparative Study on the Structural Properties and Bioactivities of Three Different Molecular Weights of <i>Lycium barbarum</i> Polysaccharides |
| title_short | Comparative Study on the Structural Properties and Bioactivities of Three Different Molecular Weights of <i>Lycium barbarum</i> Polysaccharides |
| title_sort | comparative study on the structural properties and bioactivities of three different molecular weights of i lycium barbarum i polysaccharides |
| topic | <i>Lycium barbarum</i> polysaccharides molecular weight chemical characteristics antioxidant activity immunomodulatory activity |
| url | https://www.mdpi.com/1420-3049/28/2/701 |
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