Upregulation of lncRNA LANCL1-AS1 inhibits the progression of non-small-cell lung cancer via the miR-3680-3p/GMFG axis
Patients with non-small-cell lung cancer (NSCLC) have a low survival rate. Long non-coding RNA (LncRNA) LANCL1 antisense RNA 1 (LANCL1-AS1) was indicated to be downregulated in NSCLC; however, its detailed function in NSCLC is unanswered. Real-time quantitative polymerase chain reaction revealed the...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2023-03-01
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| Series: | Open Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/med-2023-0666 |
| _version_ | 1827939317224308736 |
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| author | Pan Hui Peng Jing Qiao Xiaoni Gao Han |
| author_facet | Pan Hui Peng Jing Qiao Xiaoni Gao Han |
| author_sort | Pan Hui |
| collection | DOAJ |
| description | Patients with non-small-cell lung cancer (NSCLC) have a low survival rate. Long non-coding RNA (LncRNA) LANCL1 antisense RNA 1 (LANCL1-AS1) was indicated to be downregulated in NSCLC; however, its detailed function in NSCLC is unanswered. Real-time quantitative polymerase chain reaction revealed the downregulation of LANCL1-AS1 in NSCLC cell lines and subcellular fractionation assay showed that LANCL1-AS1 was mainly located in the cytoplasm of NSCLC cells. Cell counting kit-8, Transwell, and tube formation assays displayed that overexpression of LANCL1-AS1 suppressed NSCLC cell proliferation, migration, invasiveness, and angiogenesis in vitro. Animal experiments validated the tumor-suppressive role of LANCL1-AS1 in tumor-bearing mice. Mechanistically, LANCL1-AS1 upregulated glia maturation factor gamma (GMFG) expression by competitively binding to miR-3680-3p. GMFG knockdown reversed LANCL1-AS1 overexpression-mediated inhibitory impact on NSCLC malignant behaviors. Collectively, LANCL1-AS1 upregulation inhibits the progression of NSCLC by modulating the miR-3680-3p/GMFG axis. |
| first_indexed | 2024-03-13T08:51:48Z |
| format | Article |
| id | doaj.art-00b6cf25efe44a999e08c274ef47aaa4 |
| institution | Directory Open Access Journal |
| issn | 2391-5463 |
| language | English |
| last_indexed | 2024-03-13T08:51:48Z |
| publishDate | 2023-03-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Medicine |
| spelling | doaj.art-00b6cf25efe44a999e08c274ef47aaa42023-05-29T09:50:06ZengDe GruyterOpen Medicine2391-54632023-03-011816243310.1515/med-2023-0666Upregulation of lncRNA LANCL1-AS1 inhibits the progression of non-small-cell lung cancer via the miR-3680-3p/GMFG axisPan Hui0Peng Jing1Qiao Xiaoni2Gao Han3Department of Respiratory Medicine, Gansu Provincial Hospital, Lanzhou730000, Gansu, ChinaDepartment of Customer Service Center, Gansu Provincial Hospital, Lanzhou730000, Gansu, ChinaDepartment of Information Center, Gansu Provincial Hospital, Lanzhou730000, Gansu, ChinaDepartment of Information Center, Gansu Provincial Hospital, Lanzhou730000, Gansu, ChinaPatients with non-small-cell lung cancer (NSCLC) have a low survival rate. Long non-coding RNA (LncRNA) LANCL1 antisense RNA 1 (LANCL1-AS1) was indicated to be downregulated in NSCLC; however, its detailed function in NSCLC is unanswered. Real-time quantitative polymerase chain reaction revealed the downregulation of LANCL1-AS1 in NSCLC cell lines and subcellular fractionation assay showed that LANCL1-AS1 was mainly located in the cytoplasm of NSCLC cells. Cell counting kit-8, Transwell, and tube formation assays displayed that overexpression of LANCL1-AS1 suppressed NSCLC cell proliferation, migration, invasiveness, and angiogenesis in vitro. Animal experiments validated the tumor-suppressive role of LANCL1-AS1 in tumor-bearing mice. Mechanistically, LANCL1-AS1 upregulated glia maturation factor gamma (GMFG) expression by competitively binding to miR-3680-3p. GMFG knockdown reversed LANCL1-AS1 overexpression-mediated inhibitory impact on NSCLC malignant behaviors. Collectively, LANCL1-AS1 upregulation inhibits the progression of NSCLC by modulating the miR-3680-3p/GMFG axis.https://doi.org/10.1515/med-2023-0666non-small-cell lung cancerlancl1-as1mir-3680-3pgmfg |
| spellingShingle | Pan Hui Peng Jing Qiao Xiaoni Gao Han Upregulation of lncRNA LANCL1-AS1 inhibits the progression of non-small-cell lung cancer via the miR-3680-3p/GMFG axis Open Medicine non-small-cell lung cancer lancl1-as1 mir-3680-3p gmfg |
| title | Upregulation of lncRNA LANCL1-AS1 inhibits the progression of non-small-cell lung cancer via the miR-3680-3p/GMFG axis |
| title_full | Upregulation of lncRNA LANCL1-AS1 inhibits the progression of non-small-cell lung cancer via the miR-3680-3p/GMFG axis |
| title_fullStr | Upregulation of lncRNA LANCL1-AS1 inhibits the progression of non-small-cell lung cancer via the miR-3680-3p/GMFG axis |
| title_full_unstemmed | Upregulation of lncRNA LANCL1-AS1 inhibits the progression of non-small-cell lung cancer via the miR-3680-3p/GMFG axis |
| title_short | Upregulation of lncRNA LANCL1-AS1 inhibits the progression of non-small-cell lung cancer via the miR-3680-3p/GMFG axis |
| title_sort | upregulation of lncrna lancl1 as1 inhibits the progression of non small cell lung cancer via the mir 3680 3p gmfg axis |
| topic | non-small-cell lung cancer lancl1-as1 mir-3680-3p gmfg |
| url | https://doi.org/10.1515/med-2023-0666 |
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