Quartz Crystal Microbalance (QCM) Based Biosensor Functionalized by HER2/neu Antibody for Breast Cancer Cell Detection
The heterogeneity and metastatic features of cancer cells lead to a great number of casualties in the world. Additionally, its diagnosis as well as its treatment is highly expensive. Therefore, development of simple but effective diagnostic systems which detect the molecular markers of cancer is of...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/9/4/80 |
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| author | Merve Yılmaz Monireh Bakhshpour Ilgım Göktürk Ayşe Kevser Pişkin Adil Denizli |
| author_facet | Merve Yılmaz Monireh Bakhshpour Ilgım Göktürk Ayşe Kevser Pişkin Adil Denizli |
| author_sort | Merve Yılmaz |
| collection | DOAJ |
| description | The heterogeneity and metastatic features of cancer cells lead to a great number of casualties in the world. Additionally, its diagnosis as well as its treatment is highly expensive. Therefore, development of simple but effective diagnostic systems which detect the molecular markers of cancer is of great importance. The molecular changes on cancer cell membranes serve as targets, such as HER2/neu receptor which is detected on the surface of highly metastatic breast cancer cells. We have aimed to develop a specific and simple quartz crystal microbalance (QCM)-based system to identify HER2/neu expressing breast cancer cells via a receptor-specific monoclonal antibody. First, the QCM chip was coated with polymeric nanoparticles composed of hydroxyethylmethacrylate (HEMA) and ethylene glycol dimethacrylate (EDMA). The nanoparticle coated QCM chip was then functionalized by binding of HER2/neu antibody. The breast cancer cells with/without HER2/neu receptor expression, namely, SKBR3, MDA-MB 231 and also mouse fibroblasts were passed over the chip at a rate of 10–500 cells/mL and the mass changes (Δm) on cell/cm<sup>2</sup> unit surface of sensor were detected in real-time. The detection limit of the system was 10 cells/mL. Thus, this QCM-based HER2/neu receptor antibody functionalized system might be used effectively in the detection of HER2/neu expressing SKBR3 breast cancer cells. |
| first_indexed | 2024-03-10T12:19:09Z |
| format | Article |
| id | doaj.art-00b6f0423a6c46b2925e6cafa9bae5e3 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-10T12:19:09Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-00b6f0423a6c46b2925e6cafa9bae5e32023-11-21T15:35:24ZengMDPI AGChemosensors2227-90402021-04-01948010.3390/chemosensors9040080Quartz Crystal Microbalance (QCM) Based Biosensor Functionalized by HER2/neu Antibody for Breast Cancer Cell DetectionMerve Yılmaz0Monireh Bakhshpour1Ilgım Göktürk2Ayşe Kevser Pişkin3Adil Denizli4Medical Biochemistry Department, Faculty of Medicine, Hacettepe University, 06230 Ankara, TurkeyChemistry Department, Faculty of Science, Hacettepe University, 06230 Ankara, TurkeyChemistry Department, Faculty of Science, Hacettepe University, 06230 Ankara, TurkeyMedical Biology Department, Faculty of Medicine, Lokman Hekim University, 06230 Ankara, TurkeyChemistry Department, Faculty of Science, Hacettepe University, 06230 Ankara, TurkeyThe heterogeneity and metastatic features of cancer cells lead to a great number of casualties in the world. Additionally, its diagnosis as well as its treatment is highly expensive. Therefore, development of simple but effective diagnostic systems which detect the molecular markers of cancer is of great importance. The molecular changes on cancer cell membranes serve as targets, such as HER2/neu receptor which is detected on the surface of highly metastatic breast cancer cells. We have aimed to develop a specific and simple quartz crystal microbalance (QCM)-based system to identify HER2/neu expressing breast cancer cells via a receptor-specific monoclonal antibody. First, the QCM chip was coated with polymeric nanoparticles composed of hydroxyethylmethacrylate (HEMA) and ethylene glycol dimethacrylate (EDMA). The nanoparticle coated QCM chip was then functionalized by binding of HER2/neu antibody. The breast cancer cells with/without HER2/neu receptor expression, namely, SKBR3, MDA-MB 231 and also mouse fibroblasts were passed over the chip at a rate of 10–500 cells/mL and the mass changes (Δm) on cell/cm<sup>2</sup> unit surface of sensor were detected in real-time. The detection limit of the system was 10 cells/mL. Thus, this QCM-based HER2/neu receptor antibody functionalized system might be used effectively in the detection of HER2/neu expressing SKBR3 breast cancer cells.https://www.mdpi.com/2227-9040/9/4/80breast cancerHER2/neureceptorQCMbiosensor |
| spellingShingle | Merve Yılmaz Monireh Bakhshpour Ilgım Göktürk Ayşe Kevser Pişkin Adil Denizli Quartz Crystal Microbalance (QCM) Based Biosensor Functionalized by HER2/neu Antibody for Breast Cancer Cell Detection Chemosensors breast cancer HER2/neu receptor QCM biosensor |
| title | Quartz Crystal Microbalance (QCM) Based Biosensor Functionalized by HER2/neu Antibody for Breast Cancer Cell Detection |
| title_full | Quartz Crystal Microbalance (QCM) Based Biosensor Functionalized by HER2/neu Antibody for Breast Cancer Cell Detection |
| title_fullStr | Quartz Crystal Microbalance (QCM) Based Biosensor Functionalized by HER2/neu Antibody for Breast Cancer Cell Detection |
| title_full_unstemmed | Quartz Crystal Microbalance (QCM) Based Biosensor Functionalized by HER2/neu Antibody for Breast Cancer Cell Detection |
| title_short | Quartz Crystal Microbalance (QCM) Based Biosensor Functionalized by HER2/neu Antibody for Breast Cancer Cell Detection |
| title_sort | quartz crystal microbalance qcm based biosensor functionalized by her2 neu antibody for breast cancer cell detection |
| topic | breast cancer HER2/neu receptor QCM biosensor |
| url | https://www.mdpi.com/2227-9040/9/4/80 |
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