Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study
Objective: Disruption of the intestinal epithelial barrier has been shown to occur following burn injury. This process can lead to translocation of pathogens from the gut lumen to the systemic circulation and distant organs thereby increasing the risk for sepsis. The aim of this study was to examine...
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| Format: | Article |
| Language: | English |
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Hitit University
2021-10-01
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| Series: | Hitit Medical Journal |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/download/article-file/1869581 |
| _version_ | 1797221462875045888 |
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| author | Emre Karakaya Necip Altundaş Erdal Karagülle |
| author_facet | Emre Karakaya Necip Altundaş Erdal Karagülle |
| author_sort | Emre Karakaya |
| collection | DOAJ |
| description | Objective: Disruption of the intestinal epithelial barrier has been shown to occur following burn injury. This process can lead to translocation of pathogens from the gut lumen to the systemic circulation and distant organs thereby increasing the risk for sepsis. The aim of this study was to examine the effect of amifostine (WR-2721) on bacterial translocation in a rat burn injury model.
Material and Method: A total of 27 male Wistar albino rats were divided into three groups of nine. Group I was a control group. Group II and Group III was subjected to third-degree burns over 30% of the total body surface area, and group III was administered amifostine 200 ml/kg intraperitoneally, followed by a 10 ml/kg/day maintenance dose after undergoing third-degree burns. After 48 hours, tissue and blood samples were obtained and cultured from the liver, spleen, mesenteric lymph nodes, and cecum.
Results: Blood cultures were negative in all groups. In the control group, colonization appeared only in the cecum, but in groups II and III, colonization was found in the liver, spleen, mesenteric lymph nodes, and cecum. While bacterial colonization was most frequently found in the cecum and mesenteric lymph nodes, bacterial counts did not significantly differ in the cecum (P = 0.298) and mesenteric lymph nodes (P = 0.418) between groups II and III.
Conclusion: Amifostine alone is not effective in controlling bacterial translocation associated with burn injuries. These results should be interpreted with caution as there are a number of factors that affect bacterial translocation. |
| first_indexed | 2024-03-11T15:43:44Z |
| format | Article |
| id | doaj.art-00b776c6ef8f4d6585c11e76066e73cc |
| institution | Directory Open Access Journal |
| issn | 2687-4717 |
| language | English |
| last_indexed | 2024-04-24T13:05:50Z |
| publishDate | 2021-10-01 |
| publisher | Hitit University |
| record_format | Article |
| series | Hitit Medical Journal |
| spelling | doaj.art-00b776c6ef8f4d6585c11e76066e73cc2024-04-05T08:04:17ZengHitit UniversityHitit Medical Journal2687-47172021-10-0133717510.52827/hititmedj.967106150Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental StudyEmre Karakaya0Necip Altundaş1Erdal Karagülle2BAŞKENT ÜNİVERSİTESİATATÜRK ÜNİVERSİTESİBAŞKENT ÜNİVERSİTESİObjective: Disruption of the intestinal epithelial barrier has been shown to occur following burn injury. This process can lead to translocation of pathogens from the gut lumen to the systemic circulation and distant organs thereby increasing the risk for sepsis. The aim of this study was to examine the effect of amifostine (WR-2721) on bacterial translocation in a rat burn injury model. Material and Method: A total of 27 male Wistar albino rats were divided into three groups of nine. Group I was a control group. Group II and Group III was subjected to third-degree burns over 30% of the total body surface area, and group III was administered amifostine 200 ml/kg intraperitoneally, followed by a 10 ml/kg/day maintenance dose after undergoing third-degree burns. After 48 hours, tissue and blood samples were obtained and cultured from the liver, spleen, mesenteric lymph nodes, and cecum. Results: Blood cultures were negative in all groups. In the control group, colonization appeared only in the cecum, but in groups II and III, colonization was found in the liver, spleen, mesenteric lymph nodes, and cecum. While bacterial colonization was most frequently found in the cecum and mesenteric lymph nodes, bacterial counts did not significantly differ in the cecum (P = 0.298) and mesenteric lymph nodes (P = 0.418) between groups II and III. Conclusion: Amifostine alone is not effective in controlling bacterial translocation associated with burn injuries. These results should be interpreted with caution as there are a number of factors that affect bacterial translocation.https://dergipark.org.tr/tr/download/article-file/1869581amifostinebacterial translocationburnsyanıklaramifostinbakteriyel translokasyonyanıklar |
| spellingShingle | Emre Karakaya Necip Altundaş Erdal Karagülle Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study Hitit Medical Journal amifostine bacterial translocation burns yanıklar amifostin bakteriyel translokasyon yanıklar |
| title | Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study |
| title_full | Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study |
| title_fullStr | Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study |
| title_full_unstemmed | Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study |
| title_short | Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study |
| title_sort | effect of amifostine wr 2721 on bacterial translocation after burn injury an experimental study |
| topic | amifostine bacterial translocation burns yanıklar amifostin bakteriyel translokasyon yanıklar |
| url | https://dergipark.org.tr/tr/download/article-file/1869581 |
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