Comparative Evaluation of Protease Extraction from Leaves of <i>Syzygium polyanthum</i>
In this study, the comparison of four extraction methods for protease from the leaves of Syzygium polyanthum was evaluated. The methods include sodium phosphate buffer, 1-methyl-8-oxyquinolinium betaine (QB) solvent based extraction, hydrogen-bond acceptor (HBA) solvent based extraction and Trichlor...
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| Format: | Article |
| Language: | English |
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Department of Chemistry, Universitas Gadjah Mada
2019-04-01
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| Series: | Indonesian Journal of Chemistry |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijc/article/view/40860 |
| _version_ | 1830358029131841536 |
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| author | Noorhafiza Yahya Zatul Iffah Mohd Arshad Siti Kholijah Abdul Mudalip Siti Zubaidah Sulaiman Rohaida Che Man Shalyda Md Shaarani Mohd Hairul Ab. Rahim |
| author_facet | Noorhafiza Yahya Zatul Iffah Mohd Arshad Siti Kholijah Abdul Mudalip Siti Zubaidah Sulaiman Rohaida Che Man Shalyda Md Shaarani Mohd Hairul Ab. Rahim |
| author_sort | Noorhafiza Yahya |
| collection | DOAJ |
| description | In this study, the comparison of four extraction methods for protease from the leaves of Syzygium polyanthum was evaluated. The methods include sodium phosphate buffer, 1-methyl-8-oxyquinolinium betaine (QB) solvent based extraction, hydrogen-bond acceptor (HBA) solvent based extraction and Trichloroacetic-acetone (TCA) precipitation. The effectiveness of these extraction methods was measured based on the specific activity demonstrated by the crude protease extracted in this experiment. The results indicated that QB solvent-based extraction method could extract the crude protease from the leaves of S. polyanthum with the highest specific activity of 10.204 ± 0.329 U/mg compared to other extraction methods. |
| first_indexed | 2024-12-20T02:33:58Z |
| format | Article |
| id | doaj.art-00b9cae3923f49f2a223c2f8b15019b9 |
| institution | Directory Open Access Journal |
| issn | 1411-9420 2460-1578 |
| language | English |
| last_indexed | 2024-12-20T02:33:58Z |
| publishDate | 2019-04-01 |
| publisher | Department of Chemistry, Universitas Gadjah Mada |
| record_format | Article |
| series | Indonesian Journal of Chemistry |
| spelling | doaj.art-00b9cae3923f49f2a223c2f8b15019b92022-12-21T19:56:30ZengDepartment of Chemistry, Universitas Gadjah MadaIndonesian Journal of Chemistry1411-94202460-15782019-04-0119250351010.22146/ijc.4086023280Comparative Evaluation of Protease Extraction from Leaves of <i>Syzygium polyanthum</i>Noorhafiza Yahya0Zatul Iffah Mohd Arshad1Siti Kholijah Abdul Mudalip2Siti Zubaidah Sulaiman3Rohaida Che Man4Shalyda Md Shaarani5Mohd Hairul Ab. Rahim6Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak 26300, Gambang, Pahang, MalaysiaFaculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak 26300, Gambang, Pahang, MalaysiaFaculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak 26300, Gambang, Pahang, MalaysiaFaculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak 26300, Gambang, Pahang, MalaysiaFaculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak 26300, Gambang, Pahang, MalaysiaFaculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak 26300, Gambang, Pahang, MalaysiaFaculty of Industrial Sciences and Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak 26300, Gambang, Pahang, MalaysiaIn this study, the comparison of four extraction methods for protease from the leaves of Syzygium polyanthum was evaluated. The methods include sodium phosphate buffer, 1-methyl-8-oxyquinolinium betaine (QB) solvent based extraction, hydrogen-bond acceptor (HBA) solvent based extraction and Trichloroacetic-acetone (TCA) precipitation. The effectiveness of these extraction methods was measured based on the specific activity demonstrated by the crude protease extracted in this experiment. The results indicated that QB solvent-based extraction method could extract the crude protease from the leaves of S. polyanthum with the highest specific activity of 10.204 ± 0.329 U/mg compared to other extraction methods.https://jurnal.ugm.ac.id/ijc/article/view/40860plant proteaseprotease extractionSyzigium polyanthum |
| spellingShingle | Noorhafiza Yahya Zatul Iffah Mohd Arshad Siti Kholijah Abdul Mudalip Siti Zubaidah Sulaiman Rohaida Che Man Shalyda Md Shaarani Mohd Hairul Ab. Rahim Comparative Evaluation of Protease Extraction from Leaves of <i>Syzygium polyanthum</i> Indonesian Journal of Chemistry plant protease protease extraction Syzigium polyanthum |
| title | Comparative Evaluation of Protease Extraction from Leaves of <i>Syzygium polyanthum</i> |
| title_full | Comparative Evaluation of Protease Extraction from Leaves of <i>Syzygium polyanthum</i> |
| title_fullStr | Comparative Evaluation of Protease Extraction from Leaves of <i>Syzygium polyanthum</i> |
| title_full_unstemmed | Comparative Evaluation of Protease Extraction from Leaves of <i>Syzygium polyanthum</i> |
| title_short | Comparative Evaluation of Protease Extraction from Leaves of <i>Syzygium polyanthum</i> |
| title_sort | comparative evaluation of protease extraction from leaves of i syzygium polyanthum i |
| topic | plant protease protease extraction Syzigium polyanthum |
| url | https://jurnal.ugm.ac.id/ijc/article/view/40860 |
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