Registered report: Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion

The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of 50 papers in the field of cancer biology published between 2010 and 2012. This Registered Report describes the proposed replication plan of key exp...

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Main Authors: David Blum, Samuel LaBarge, Reproducibility Project: Cancer Biology
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2014-12-01
Series:eLife
Subjects:
Online Access:https://elifesciences.org/articles/04034
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author David Blum
Samuel LaBarge
Reproducibility Project: Cancer Biology
author_facet David Blum
Samuel LaBarge
Reproducibility Project: Cancer Biology
author_sort David Blum
collection DOAJ
description The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of 50 papers in the field of cancer biology published between 2010 and 2012. This Registered Report describes the proposed replication plan of key experiments from ‘Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion’ by Straussman and colleagues, published in Nature in 2012 (Straussman et al., 2012). The key experiments being replicated in this study are from Figure 2A, C, and D (and Supplemental Figure 11) and Figure 4C (and Supplemental Figure 19) (Straussman et al., 2012). Figure 2 demonstrates resistance to drug sensitivity conferred by co-culture with some stromal cell lines and identifies the secreted factor responsible as HGF. In Figure 4, Straussman and colleagues show that blocking the HGF receptor MET abrogates HGF’s rescue of drug sensitivity. The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Science and Science Exchange, and the results of the replications will be published by eLife.
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spelling doaj.art-00bc28e415b146678905d141bebde1812022-12-22T04:32:17ZengeLife Sciences Publications LtdeLife2050-084X2014-12-01310.7554/eLife.04034Registered report: Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretionDavid Blum0Samuel LaBarge1Reproducibility Project: Cancer BiologyBioexpression and Fermentation Facility, University of Georgia, Athens, United StatesCity of Hope, Duarte, United StatesThe Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of 50 papers in the field of cancer biology published between 2010 and 2012. This Registered Report describes the proposed replication plan of key experiments from ‘Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion’ by Straussman and colleagues, published in Nature in 2012 (Straussman et al., 2012). The key experiments being replicated in this study are from Figure 2A, C, and D (and Supplemental Figure 11) and Figure 4C (and Supplemental Figure 19) (Straussman et al., 2012). Figure 2 demonstrates resistance to drug sensitivity conferred by co-culture with some stromal cell lines and identifies the secreted factor responsible as HGF. In Figure 4, Straussman and colleagues show that blocking the HGF receptor MET abrogates HGF’s rescue of drug sensitivity. The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Science and Science Exchange, and the results of the replications will be published by eLife.https://elifesciences.org/articles/04034Reproducibility Project: Cancer BiologymethodologyRTK inhibitor resistance
spellingShingle David Blum
Samuel LaBarge
Reproducibility Project: Cancer Biology
Registered report: Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion
eLife
Reproducibility Project: Cancer Biology
methodology
RTK inhibitor resistance
title Registered report: Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion
title_full Registered report: Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion
title_fullStr Registered report: Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion
title_full_unstemmed Registered report: Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion
title_short Registered report: Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion
title_sort registered report tumour micro environment elicits innate resistance to raf inhibitors through hgf secretion
topic Reproducibility Project: Cancer Biology
methodology
RTK inhibitor resistance
url https://elifesciences.org/articles/04034
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