CRISPR/Cas9-mediated genome editing in vancomycin-producing strain Amycolatopsis keratiniphila

CRISPR/Cas9-mediated genome editing in vancomycin-producing strain Amycolatopsis keratiniphila

Amycolatopsis is an important source of diverse valuable bioactive natural products. The CRISPR/Cas-mediated gene editing tool has been established in some Amycolatopsis species and has accomplished the deletion of single gene or two genes. The goal of this study was to develop a high-efficient CRIS...

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Bibliographic Details
Main Authors: Mengyi Hu, Shuo Chen, Yao Ni, Wei Wei, Wenwei Mao, Mei Ge, Xiuping Qian
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-03-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
amycolatopsis
CRISPR/Cas9
genome editing
large fragment deletion
Eco-0501
vancomycin
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2023.1141176/full

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