Nexrutine® preserves muscle mass similar to exercise in prostate cancer mouse model
Abstract Muscle loss is a debilitating side effect to prostate cancer (PCa) experienced by nearly 60% of men. The purpose of this study was to test the hypothesis that Nexrutine®, a bark extract from the Phellodendrum amurense, can protect against prostate cancer induced muscle loss in a similar man...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-08-01
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| Series: | Physiological Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.14814/phy2.14217 |
| _version_ | 1811224351162236928 |
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| author | Darpan I. Patel Derek Wallace Kira Abuchowski Paul Rivas Amber Gallegos Nicolas Musi Addanki Pratap. Kumar |
| author_facet | Darpan I. Patel Derek Wallace Kira Abuchowski Paul Rivas Amber Gallegos Nicolas Musi Addanki Pratap. Kumar |
| author_sort | Darpan I. Patel |
| collection | DOAJ |
| description | Abstract Muscle loss is a debilitating side effect to prostate cancer (PCa) experienced by nearly 60% of men. The purpose of this study was to test the hypothesis that Nexrutine®, a bark extract from the Phellodendrum amurense, can protect against prostate cancer induced muscle loss in a similar manner as exercise, using the transgenic adenocarcinoma of mouse prostate (TRAMP) model. Forty‐five, 8‐ to 10‐week old TRAMP mice were randomized to either control, Nexrutine® (600 mg/kg pelleted in chow) or exercise (voluntary wheel running). Mice were serially sacrificed at weeks 4, 8, 12, and 20, at which time either the left or right gastrocnemius muscle was harvested, weighted, and frozen. Proteolysis inducing factor (PIF), ubiquitin, and NF‐κB concentrations were quantified using ELISA kits. Nexrutine® and exercise were equally able to protect TRAMP mice against PCa‐induced muscle loss (P = 0.04). Both interventions decreased intramuscular PIF concentrations at 20 weeks compared to control (P < 0.05). A treatment effect was also observed when all time points were combined with exercise significantly lowering PIF concentrations (P < 0.01). Exercise significantly lowered intramuscular ubiquitin concentrations in weeks 4, 8, and 20 compared to control mice (P < 0.001). A treatment effect was also observed with exercise significantly lowering ubiquitin compared to control mice (P < 0.001). No significant changes were observed for NF‐κB. The results of this investigation demonstrate that PCa‐induced muscle loss can be attenuated with the herbal supplement Nexrutine®. This investigation provides preliminary evidence to support continued research into Nexrutine® as a potential exercise analog in protecting against muscle loss. |
| first_indexed | 2024-04-12T08:47:54Z |
| format | Article |
| id | doaj.art-00bf1b87b0fa4220a62981c7a6f3ecd8 |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-04-12T08:47:54Z |
| publishDate | 2019-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-00bf1b87b0fa4220a62981c7a6f3ecd82022-12-22T03:39:39ZengWileyPhysiological Reports2051-817X2019-08-01716n/an/a10.14814/phy2.14217Nexrutine® preserves muscle mass similar to exercise in prostate cancer mouse modelDarpan I. Patel0Derek Wallace1Kira Abuchowski2Paul Rivas3Amber Gallegos4Nicolas Musi5Addanki Pratap. Kumar6School of Nursing University of Texas Health Science Center at San Antonio San Antonio TexasSchool of Nursing University of Texas Health Science Center at San Antonio San Antonio TexasSchool of Nursing University of Texas Health Science Center at San Antonio San Antonio TexasDepartment of Urology, School of Medicine University of Texas Health Science Center at San Antonio San Antonio TexasSchool of Nursing University of Texas Health Science Center at San Antonio San Antonio TexasBarshop Institute for Aging University of Texas Health Science Center at San Antonio San Antonio TexasMays Cancer Center at UT Health San Antonio San Antonio TexasAbstract Muscle loss is a debilitating side effect to prostate cancer (PCa) experienced by nearly 60% of men. The purpose of this study was to test the hypothesis that Nexrutine®, a bark extract from the Phellodendrum amurense, can protect against prostate cancer induced muscle loss in a similar manner as exercise, using the transgenic adenocarcinoma of mouse prostate (TRAMP) model. Forty‐five, 8‐ to 10‐week old TRAMP mice were randomized to either control, Nexrutine® (600 mg/kg pelleted in chow) or exercise (voluntary wheel running). Mice were serially sacrificed at weeks 4, 8, 12, and 20, at which time either the left or right gastrocnemius muscle was harvested, weighted, and frozen. Proteolysis inducing factor (PIF), ubiquitin, and NF‐κB concentrations were quantified using ELISA kits. Nexrutine® and exercise were equally able to protect TRAMP mice against PCa‐induced muscle loss (P = 0.04). Both interventions decreased intramuscular PIF concentrations at 20 weeks compared to control (P < 0.05). A treatment effect was also observed when all time points were combined with exercise significantly lowering PIF concentrations (P < 0.01). Exercise significantly lowered intramuscular ubiquitin concentrations in weeks 4, 8, and 20 compared to control mice (P < 0.001). A treatment effect was also observed with exercise significantly lowering ubiquitin compared to control mice (P < 0.001). No significant changes were observed for NF‐κB. The results of this investigation demonstrate that PCa‐induced muscle loss can be attenuated with the herbal supplement Nexrutine®. This investigation provides preliminary evidence to support continued research into Nexrutine® as a potential exercise analog in protecting against muscle loss.https://doi.org/10.14814/phy2.14217atrophyinflammationnatural productneoplasm of the prostateproteolysis‐inducing factor |
| spellingShingle | Darpan I. Patel Derek Wallace Kira Abuchowski Paul Rivas Amber Gallegos Nicolas Musi Addanki Pratap. Kumar Nexrutine® preserves muscle mass similar to exercise in prostate cancer mouse model Physiological Reports atrophy inflammation natural product neoplasm of the prostate proteolysis‐inducing factor |
| title | Nexrutine® preserves muscle mass similar to exercise in prostate cancer mouse model |
| title_full | Nexrutine® preserves muscle mass similar to exercise in prostate cancer mouse model |
| title_fullStr | Nexrutine® preserves muscle mass similar to exercise in prostate cancer mouse model |
| title_full_unstemmed | Nexrutine® preserves muscle mass similar to exercise in prostate cancer mouse model |
| title_short | Nexrutine® preserves muscle mass similar to exercise in prostate cancer mouse model |
| title_sort | nexrutine r preserves muscle mass similar to exercise in prostate cancer mouse model |
| topic | atrophy inflammation natural product neoplasm of the prostate proteolysis‐inducing factor |
| url | https://doi.org/10.14814/phy2.14217 |
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