The alteration of intrauterine microbiota in chronic endometritis patients based on 16S rRNA sequencing analysis
Abstract Background Chronic endometritis (CE) is a disease of continuous and subtle inflammation occurring in the endometrial stromal area, which is often asymptomatic or present with non-specific clinical symptoms. Methods This study investigated the composition and distribution of the intrauterine...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
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| Online Access: | https://doi.org/10.1186/s12941-023-00556-4 |
| _version_ | 1797952597431156736 |
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| author | Qing Chen Xiaowei Zhang Qicai Hu Wei Zhang Yi Xie Weixia Wei |
| author_facet | Qing Chen Xiaowei Zhang Qicai Hu Wei Zhang Yi Xie Weixia Wei |
| author_sort | Qing Chen |
| collection | DOAJ |
| description | Abstract Background Chronic endometritis (CE) is a disease of continuous and subtle inflammation occurring in the endometrial stromal area, which is often asymptomatic or present with non-specific clinical symptoms. Methods This study investigated the composition and distribution of the intrauterine microbiota of 71 patients who underwent hysteroscopy during the routine clinical inspection of infertility. Among them, patients who were diagnosed with chronic endometritis (CE) were allocated into CE group (n = 29) and others into non-CE group (n = 42). There was no significant difference in average age between the two groups (P = 0.19). Uterine flushing fluid was collected by the self-developed cervical trocar uterine cavity sampler and 16S rRNA sequencing was performed. Results The alpha diversity in the CE group was significantly higher than that in the non-CE group (P < 0.05). Firmicutes (newly named Bacillota) were the dominant phylum in the non-CE group (72.23%), while their abundance was much lower in the CE group (49.92%), but there was no statistically significant difference between the two groups. The abundances of Actinobacteriota and Cyanobacteria in the CE group were significantly higher than those in the non-CE group (P < 0.05). At the genus level, the abundance of Lactobacillus dominated in all samples, which presented a significantly lower abundance in the CE group (40.88%) than that in the non-CE group (64.22%) (P < 0.05). Correspondingly, the abundance of non-Lactobacillus was higher in the CE group, among which Pseudomonas and Cutibacterium increased significantly (P < 0.01). Moreover, compared with the non-CE group, the pathways involved in arginine and proline metabolism and retinol metabolism were significantly enriched in the CE group (P < 0.05), while the metabolism of lipid and prenyltransferases were significantly decreased in the CE group (P < 0.05). Conclusions A certain microbial community was colonized in the uterine cavity, which was dominated by Lactobacillus. The structure and distribution of intrauterine microbiota in the CE group were different from those in the non-CE group by showing a lower abundance of Lactobacillus, and a significantly higher abundance of Pseudomonas and Cutibacterium. Additionally, the microbial metabolism was altered in the CE group. This study elaborated the alteration of intrauterine microbiota in CE patients, which may contribute to the diagnosis of CE and provide a reference for antibiotic treatment of CE. |
| first_indexed | 2024-04-10T22:48:49Z |
| format | Article |
| id | doaj.art-00c0536a72ca44ce883d866c63bc1f50 |
| institution | Directory Open Access Journal |
| issn | 1476-0711 |
| language | English |
| last_indexed | 2024-04-10T22:48:49Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
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| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj.art-00c0536a72ca44ce883d866c63bc1f502023-01-15T12:06:38ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112023-01-0122111010.1186/s12941-023-00556-4The alteration of intrauterine microbiota in chronic endometritis patients based on 16S rRNA sequencing analysisQing Chen0Xiaowei Zhang1Qicai Hu2Wei Zhang3Yi Xie4Weixia Wei5Department of Obstetrics and Gynecology, Peking University Shenzhen HospitalDepartment of Obstetrics and Gynecology, Peking University Shenzhen HospitalDepartment of Obstetrics and Gynecology, Peking University Shenzhen HospitalDepartment of Obstetrics and Gynecology, Peking University Shenzhen HospitalDepartment of Obstetrics and Gynecology, Peking University Shenzhen HospitalDepartment of Obstetrics and Gynecology, Peking University Shenzhen HospitalAbstract Background Chronic endometritis (CE) is a disease of continuous and subtle inflammation occurring in the endometrial stromal area, which is often asymptomatic or present with non-specific clinical symptoms. Methods This study investigated the composition and distribution of the intrauterine microbiota of 71 patients who underwent hysteroscopy during the routine clinical inspection of infertility. Among them, patients who were diagnosed with chronic endometritis (CE) were allocated into CE group (n = 29) and others into non-CE group (n = 42). There was no significant difference in average age between the two groups (P = 0.19). Uterine flushing fluid was collected by the self-developed cervical trocar uterine cavity sampler and 16S rRNA sequencing was performed. Results The alpha diversity in the CE group was significantly higher than that in the non-CE group (P < 0.05). Firmicutes (newly named Bacillota) were the dominant phylum in the non-CE group (72.23%), while their abundance was much lower in the CE group (49.92%), but there was no statistically significant difference between the two groups. The abundances of Actinobacteriota and Cyanobacteria in the CE group were significantly higher than those in the non-CE group (P < 0.05). At the genus level, the abundance of Lactobacillus dominated in all samples, which presented a significantly lower abundance in the CE group (40.88%) than that in the non-CE group (64.22%) (P < 0.05). Correspondingly, the abundance of non-Lactobacillus was higher in the CE group, among which Pseudomonas and Cutibacterium increased significantly (P < 0.01). Moreover, compared with the non-CE group, the pathways involved in arginine and proline metabolism and retinol metabolism were significantly enriched in the CE group (P < 0.05), while the metabolism of lipid and prenyltransferases were significantly decreased in the CE group (P < 0.05). Conclusions A certain microbial community was colonized in the uterine cavity, which was dominated by Lactobacillus. The structure and distribution of intrauterine microbiota in the CE group were different from those in the non-CE group by showing a lower abundance of Lactobacillus, and a significantly higher abundance of Pseudomonas and Cutibacterium. Additionally, the microbial metabolism was altered in the CE group. This study elaborated the alteration of intrauterine microbiota in CE patients, which may contribute to the diagnosis of CE and provide a reference for antibiotic treatment of CE.https://doi.org/10.1186/s12941-023-00556-4Chronic endometritisIntrauterine microbiota16S rRNA sequencingFirmicutes (Bacillota)Lactobacillus |
| spellingShingle | Qing Chen Xiaowei Zhang Qicai Hu Wei Zhang Yi Xie Weixia Wei The alteration of intrauterine microbiota in chronic endometritis patients based on 16S rRNA sequencing analysis Annals of Clinical Microbiology and Antimicrobials Chronic endometritis Intrauterine microbiota 16S rRNA sequencing Firmicutes (Bacillota) Lactobacillus |
| title | The alteration of intrauterine microbiota in chronic endometritis patients based on 16S rRNA sequencing analysis |
| title_full | The alteration of intrauterine microbiota in chronic endometritis patients based on 16S rRNA sequencing analysis |
| title_fullStr | The alteration of intrauterine microbiota in chronic endometritis patients based on 16S rRNA sequencing analysis |
| title_full_unstemmed | The alteration of intrauterine microbiota in chronic endometritis patients based on 16S rRNA sequencing analysis |
| title_short | The alteration of intrauterine microbiota in chronic endometritis patients based on 16S rRNA sequencing analysis |
| title_sort | alteration of intrauterine microbiota in chronic endometritis patients based on 16s rrna sequencing analysis |
| topic | Chronic endometritis Intrauterine microbiota 16S rRNA sequencing Firmicutes (Bacillota) Lactobacillus |
| url | https://doi.org/10.1186/s12941-023-00556-4 |
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