Dihydroneopterin and the generation of superoxide from iron ions
We describe the impact of dihydroneopterin on the formation of superoxide radicals. As radical source ferrous iron in air-saturated aequeous solution was used. Detection of superoxide was achieved using reduction of nitroblue tetrazolium to formazan. Dihydroneopterin decreases formazan formation whe...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2000-05-01
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| Series: | Pteridines |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/pteridines.2000.11.2.60 |
| _version_ | 1818695818156179456 |
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| author | Oettl Karl Greilberger Joachim Reibnegger Gilbert |
| author_facet | Oettl Karl Greilberger Joachim Reibnegger Gilbert |
| author_sort | Oettl Karl |
| collection | DOAJ |
| description | We describe the impact of dihydroneopterin on the formation of superoxide radicals. As radical source ferrous iron in air-saturated aequeous solution was used. Detection of superoxide was achieved using reduction of nitroblue tetrazolium to formazan. Dihydroneopterin decreases formazan formation when high concentrations of ferrous iron are used which induce a rapid superoxide formation within minutes. On the other hand, when low concentrations of ferrous iron are used, addition of dihydroneopterin to the assay mixture leads to an increase ir. formazan formation for several hours of incubation. We therefore conclude that the radical promoting activity of dihydroneopterin is physiologically more relevant compared to its radical scavenging properties. |
| first_indexed | 2024-12-17T13:51:31Z |
| format | Article |
| id | doaj.art-00c20a361043458b9fb5cb6b11bf7cf1 |
| institution | Directory Open Access Journal |
| issn | 0933-4807 2195-4720 |
| language | English |
| last_indexed | 2024-12-17T13:51:31Z |
| publishDate | 2000-05-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Pteridines |
| spelling | doaj.art-00c20a361043458b9fb5cb6b11bf7cf12022-12-21T21:46:01ZengDe GruyterPteridines0933-48072195-47202000-05-01112606310.1515/pteridines.2000.11.2.60Dihydroneopterin and the generation of superoxide from iron ionsOettl Karl0Greilberger Joachim1Reibnegger Gilbert2Institute for Medical Chernisty and Pregl-Laboratory, Karl-Franzens-University Graz, Harrachgasse 21111, A· 8010 Graz, AustriaInstitute for Medical Chernisty and Pregl-Laboratory, Karl-Franzens-University Graz, Harrachgasse 21111, A· 8010 Graz, AustriaInstitute for Medical Chernisty and Pregl-Laboratory, Karl-Franzens-University Graz, Harrachgasse 21111, A· 8010 Graz, AustriaWe describe the impact of dihydroneopterin on the formation of superoxide radicals. As radical source ferrous iron in air-saturated aequeous solution was used. Detection of superoxide was achieved using reduction of nitroblue tetrazolium to formazan. Dihydroneopterin decreases formazan formation when high concentrations of ferrous iron are used which induce a rapid superoxide formation within minutes. On the other hand, when low concentrations of ferrous iron are used, addition of dihydroneopterin to the assay mixture leads to an increase ir. formazan formation for several hours of incubation. We therefore conclude that the radical promoting activity of dihydroneopterin is physiologically more relevant compared to its radical scavenging properties.https://doi.org/10.1515/pteridines.2000.11.2.60oxygen speciesnitroblue tetrazoliumfolmazaniron |
| spellingShingle | Oettl Karl Greilberger Joachim Reibnegger Gilbert Dihydroneopterin and the generation of superoxide from iron ions Pteridines oxygen species nitroblue tetrazolium folmazan iron |
| title | Dihydroneopterin and the generation of superoxide from iron ions |
| title_full | Dihydroneopterin and the generation of superoxide from iron ions |
| title_fullStr | Dihydroneopterin and the generation of superoxide from iron ions |
| title_full_unstemmed | Dihydroneopterin and the generation of superoxide from iron ions |
| title_short | Dihydroneopterin and the generation of superoxide from iron ions |
| title_sort | dihydroneopterin and the generation of superoxide from iron ions |
| topic | oxygen species nitroblue tetrazolium folmazan iron |
| url | https://doi.org/10.1515/pteridines.2000.11.2.60 |
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