Research Note: Cecal microbiota harbored by free-range chickens may influence the reduction of Helicobacter pullorum relative abundance
ABSTRACT: Helicobacter pullorum is recognized as an emerging food-borne pathogen that may colonize the intestinal tract and the liver of avian species and humans causing several gastrointestinal and liver diseases. However, not all strains are reported to be capable of causing clinical disease, thus...
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-02-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122005181 |
| _version_ | 1811175520103038976 |
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| author | Luca Borrelli Lorena Varriale Lorena Coretti Antonino Pace Tamara Pasqualina Russo Antonio Santaniello Luigi Gavazzi Alessandro Fioretti Ludovico Dipineto |
| author_facet | Luca Borrelli Lorena Varriale Lorena Coretti Antonino Pace Tamara Pasqualina Russo Antonio Santaniello Luigi Gavazzi Alessandro Fioretti Ludovico Dipineto |
| author_sort | Luca Borrelli |
| collection | DOAJ |
| description | ABSTRACT: Helicobacter pullorum is recognized as an emerging food-borne pathogen that may colonize the intestinal tract and the liver of avian species and humans causing several gastrointestinal and liver diseases. However, not all strains are reported to be capable of causing clinical disease, thus making poultry as reservoir for the zoonotic transmission of the infection through carcass contamination of broilers at slaughter. In poultry, the prevalence of this bacterium could be underestimated and the available data mainly refer to conventional rearing systems, whereas free-range and organic breedings have been poorly investigated. Therefore, this study was aimed to characterize the caecal microbiota community of free-range grown chickens and determine the presence and the relative abundance of H. pullorum by using NGS-based 16S rDNA sequencing. A total of 18 chickens reared for 56 d on a semi-extensive management system were euthanized at two time points: 9 birds at 28 d of age (before have access to outdoor; I = Indoor) and other 9 birds at 56 d of age (before slaughter; O = Outdoor). Cecal contents were collected for microbiota analyses. H. pullorum was detected in the cecum of 16/18 samples and its proportion in indoor was significantly higher than outdoor chickens (2.46 and 0.52%, respectively; P < 0.05), showing 78.8% of decrease with the outdoor access of the chickens. Therefore, it may be assumed that the potential for zoonotic infection is less likely. Moreover, H. pullorum was negatively correlated with 17 bacterial species as significantly more abundant in Outdoor microbial caecal communities. Among these, we highlighted the presence of Mucispirillium schaedleri and Oscillospira, already previously associated with a healthy gut and thus representing promising gut bacterial markers for host health. Our findings suggest that alternative production systems with outdoor access, may play a crucial role in the establishment of a healthy gut microbiota, which in turn might prevent colonization of harmful bacteria such as Helicobacter pullorum. |
| first_indexed | 2024-04-10T19:37:14Z |
| format | Article |
| id | doaj.art-00c25ea5c1b44729b6648e3b5e56a71f |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-10T19:37:14Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-00c25ea5c1b44729b6648e3b5e56a71f2023-01-30T04:11:06ZengElsevierPoultry Science0032-57912023-02-011022102222Research Note: Cecal microbiota harbored by free-range chickens may influence the reduction of Helicobacter pullorum relative abundanceLuca Borrelli0Lorena Varriale1Lorena Coretti2Antonino Pace3Tamara Pasqualina Russo4Antonio Santaniello5Luigi Gavazzi6Alessandro Fioretti7Ludovico Dipineto8Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy; Task Force on Microbiome Studies, University of Naples Federico II, Naples, Italy; Corresponding author:Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, ItalyTask Force on Microbiome Studies, University of Naples Federico II, Naples, Italy; Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Naples, ItalyDepartment of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, ItalyDepartment of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, ItalyDepartment of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, ItalyVeterinary Practice of Poultry Sector, 25100 Brescia, ItalyDepartment of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, ItalyDepartment of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy; Task Force on Microbiome Studies, University of Naples Federico II, Naples, ItalyABSTRACT: Helicobacter pullorum is recognized as an emerging food-borne pathogen that may colonize the intestinal tract and the liver of avian species and humans causing several gastrointestinal and liver diseases. However, not all strains are reported to be capable of causing clinical disease, thus making poultry as reservoir for the zoonotic transmission of the infection through carcass contamination of broilers at slaughter. In poultry, the prevalence of this bacterium could be underestimated and the available data mainly refer to conventional rearing systems, whereas free-range and organic breedings have been poorly investigated. Therefore, this study was aimed to characterize the caecal microbiota community of free-range grown chickens and determine the presence and the relative abundance of H. pullorum by using NGS-based 16S rDNA sequencing. A total of 18 chickens reared for 56 d on a semi-extensive management system were euthanized at two time points: 9 birds at 28 d of age (before have access to outdoor; I = Indoor) and other 9 birds at 56 d of age (before slaughter; O = Outdoor). Cecal contents were collected for microbiota analyses. H. pullorum was detected in the cecum of 16/18 samples and its proportion in indoor was significantly higher than outdoor chickens (2.46 and 0.52%, respectively; P < 0.05), showing 78.8% of decrease with the outdoor access of the chickens. Therefore, it may be assumed that the potential for zoonotic infection is less likely. Moreover, H. pullorum was negatively correlated with 17 bacterial species as significantly more abundant in Outdoor microbial caecal communities. Among these, we highlighted the presence of Mucispirillium schaedleri and Oscillospira, already previously associated with a healthy gut and thus representing promising gut bacterial markers for host health. Our findings suggest that alternative production systems with outdoor access, may play a crucial role in the establishment of a healthy gut microbiota, which in turn might prevent colonization of harmful bacteria such as Helicobacter pullorum.http://www.sciencedirect.com/science/article/pii/S0032579122005181Helicobacter pullorumfree-range chickenceacal microbiotazoonoses |
| spellingShingle | Luca Borrelli Lorena Varriale Lorena Coretti Antonino Pace Tamara Pasqualina Russo Antonio Santaniello Luigi Gavazzi Alessandro Fioretti Ludovico Dipineto Research Note: Cecal microbiota harbored by free-range chickens may influence the reduction of Helicobacter pullorum relative abundance Poultry Science Helicobacter pullorum free-range chicken ceacal microbiota zoonoses |
| title | Research Note: Cecal microbiota harbored by free-range chickens may influence the reduction of Helicobacter pullorum relative abundance |
| title_full | Research Note: Cecal microbiota harbored by free-range chickens may influence the reduction of Helicobacter pullorum relative abundance |
| title_fullStr | Research Note: Cecal microbiota harbored by free-range chickens may influence the reduction of Helicobacter pullorum relative abundance |
| title_full_unstemmed | Research Note: Cecal microbiota harbored by free-range chickens may influence the reduction of Helicobacter pullorum relative abundance |
| title_short | Research Note: Cecal microbiota harbored by free-range chickens may influence the reduction of Helicobacter pullorum relative abundance |
| title_sort | research note cecal microbiota harbored by free range chickens may influence the reduction of helicobacter pullorum relative abundance |
| topic | Helicobacter pullorum free-range chicken ceacal microbiota zoonoses |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122005181 |
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