The context signals of mitochondrial miRNAs (mitomiRs) of mammals
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level in the cytoplasm and play an important role in a wide range of biological processes. Recent studies have found that the miRNA sequences are presented not only in the cytoplasm, but also in th...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2023-01-01
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| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/3584 |
| _version_ | 1797213917338927104 |
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| author | O. V. Vishnevsky P. S. Vorozheykin I. I. Titov |
| author_facet | O. V. Vishnevsky P. S. Vorozheykin I. I. Titov |
| author_sort | O. V. Vishnevsky |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level in the cytoplasm and play an important role in a wide range of biological processes. Recent studies have found that the miRNA sequences are presented not only in the cytoplasm, but also in the mitochondria. These miRNAs (the so-called mitomiRs) may be the sequences of nuclear or mitochondrial origin; some of them are involved in regulation of the mitochondrial gene functions, while the role of others is still unknown. The identification of nucleotide signals, which are unique to mitomiRs, may help to determine this role. We formed a dataset that combined the experimentally discovered mitomiRs in human, rat and mouse. To isolate signals that may be responsible for the mitomiRs’ functions or for their translocation from or into mitochondria a context analysis was carried out for the sequences. For three species in the group mitomiRs/non-mitomiRs and the group of all miRNAs from the miRBase database statistically overrepresented 8-letter motifs were identified (p-value < 0.01 with Bonferroni correction for multiple comparisons), for these motifs the patterns of the localization in functionally important regions for different types of miRNAs were found. Also, for the group mitomiRs/non-mitomiRs we found the statistically significant features of the miRNA nucleotide context near the Dicer and Drosha cleavage sites (Pearson’s χ2 test of independence for the first three positions of the miRNA, p-value < 0.05). The observed nucleotide frequencies may indicate a more homogeneous pri-miRNA cleavage by the Drosha complex during the formation of the 5’ end of mitomiRs. The obtained results can help to determine the role of the nucleotide signals in the origin, processing, and functions of the mitomiRs. |
| first_indexed | 2024-03-07T16:04:40Z |
| format | Article |
| id | doaj.art-00c2bb41763e499d8cf4fd099c818412 |
| institution | Directory Open Access Journal |
| issn | 2500-3259 |
| language | English |
| last_indexed | 2024-04-24T11:05:54Z |
| publishDate | 2023-01-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj.art-00c2bb41763e499d8cf4fd099c8184122024-04-11T15:31:05ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592023-01-0126881982510.18699/VJGB-22-991321The context signals of mitochondrial miRNAs (mitomiRs) of mammalsO. V. Vishnevsky0P. S. Vorozheykin1I. I. Titov2Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesNovosibirsk State UniversityInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Novosibirsk State University; Kurchatov Genomic Center of ICG SB RASMicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level in the cytoplasm and play an important role in a wide range of biological processes. Recent studies have found that the miRNA sequences are presented not only in the cytoplasm, but also in the mitochondria. These miRNAs (the so-called mitomiRs) may be the sequences of nuclear or mitochondrial origin; some of them are involved in regulation of the mitochondrial gene functions, while the role of others is still unknown. The identification of nucleotide signals, which are unique to mitomiRs, may help to determine this role. We formed a dataset that combined the experimentally discovered mitomiRs in human, rat and mouse. To isolate signals that may be responsible for the mitomiRs’ functions or for their translocation from or into mitochondria a context analysis was carried out for the sequences. For three species in the group mitomiRs/non-mitomiRs and the group of all miRNAs from the miRBase database statistically overrepresented 8-letter motifs were identified (p-value < 0.01 with Bonferroni correction for multiple comparisons), for these motifs the patterns of the localization in functionally important regions for different types of miRNAs were found. Also, for the group mitomiRs/non-mitomiRs we found the statistically significant features of the miRNA nucleotide context near the Dicer and Drosha cleavage sites (Pearson’s χ2 test of independence for the first three positions of the miRNA, p-value < 0.05). The observed nucleotide frequencies may indicate a more homogeneous pri-miRNA cleavage by the Drosha complex during the formation of the 5’ end of mitomiRs. The obtained results can help to determine the role of the nucleotide signals in the origin, processing, and functions of the mitomiRs.https://vavilov.elpub.ru/jour/article/view/3584mirnapre-mirnamitomirmitochondrion |
| spellingShingle | O. V. Vishnevsky P. S. Vorozheykin I. I. Titov The context signals of mitochondrial miRNAs (mitomiRs) of mammals Вавиловский журнал генетики и селекции mirna pre-mirna mitomir mitochondrion |
| title | The context signals of mitochondrial miRNAs (mitomiRs) of mammals |
| title_full | The context signals of mitochondrial miRNAs (mitomiRs) of mammals |
| title_fullStr | The context signals of mitochondrial miRNAs (mitomiRs) of mammals |
| title_full_unstemmed | The context signals of mitochondrial miRNAs (mitomiRs) of mammals |
| title_short | The context signals of mitochondrial miRNAs (mitomiRs) of mammals |
| title_sort | context signals of mitochondrial mirnas mitomirs of mammals |
| topic | mirna pre-mirna mitomir mitochondrion |
| url | https://vavilov.elpub.ru/jour/article/view/3584 |
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