Inhibition of TRPP3 by calmodulin through Ca2+/calmodulin-dependent protein kinase II
Transient receptor potential (TRP) polycystin-3 (TRPP3) is a non-selective cation channel activated by Ca2+ and protons and is involved in regulating ciliary Ca2+ concentration, hedgehog signaling and sour tasting. The TRPP3 channel function and regulation are still not well understood. Here we inve...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-04-01
|
| Series: | Cell Insight |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772892723000123 |
| _version_ | 1797903286714499072 |
|---|---|
| author | Xiong Liu Yifang Wang Ziyi Weng Qinyi Xu Cefan Zhou JingFeng Tang Xing-Zhen Chen |
| author_facet | Xiong Liu Yifang Wang Ziyi Weng Qinyi Xu Cefan Zhou JingFeng Tang Xing-Zhen Chen |
| author_sort | Xiong Liu |
| collection | DOAJ |
| description | Transient receptor potential (TRP) polycystin-3 (TRPP3) is a non-selective cation channel activated by Ca2+ and protons and is involved in regulating ciliary Ca2+ concentration, hedgehog signaling and sour tasting. The TRPP3 channel function and regulation are still not well understood. Here we investigated regulation of TRPP3 by calmodulin (CaM) by means of electrophysiology and Xenopus oocytes as an expression model. We found that TRPP3 channel function is enhanced by calmidazolium, a CaM antagonist, and inhibited by CaM through binding of the CaM N-lobe to a TRPP3 C-terminal domain not overlapped with the EF-hand. We further revealed that the TRPP3/CaM interaction promotes phosphorylation of TRPP3 at threonine 591 by Ca2+/CaM-dependent protein kinase II, which mediates the inhibition of TRPP3 by CaM. |
| first_indexed | 2024-04-10T09:30:29Z |
| format | Article |
| id | doaj.art-00c587e925fa4d8a9f15d398bb50760b |
| institution | Directory Open Access Journal |
| issn | 2772-8927 |
| language | English |
| last_indexed | 2024-04-10T09:30:29Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Insight |
| spelling | doaj.art-00c587e925fa4d8a9f15d398bb50760b2023-02-19T04:27:47ZengElsevierCell Insight2772-89272023-04-0122100088Inhibition of TRPP3 by calmodulin through Ca2+/calmodulin-dependent protein kinase IIXiong Liu0Yifang Wang1Ziyi Weng2Qinyi Xu3Cefan Zhou4JingFeng Tang5Xing-Zhen Chen6Membrane Protein Disease Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, T6G 2H7, Edmonton, AB, CanadaMembrane Protein Disease Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, T6G 2H7, Edmonton, AB, Canada; National “111'' Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan, Hubei, 430068, ChinaMembrane Protein Disease Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, T6G 2H7, Edmonton, AB, Canada; National “111'' Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan, Hubei, 430068, ChinaMembrane Protein Disease Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, T6G 2H7, Edmonton, AB, CanadaNational “111'' Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan, Hubei, 430068, ChinaNational “111'' Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan, Hubei, 430068, China; Corresponding author. National ''111'' Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan, Hubei, 430068, China.Membrane Protein Disease Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, T6G 2H7, Edmonton, AB, Canada; National “111'' Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan, Hubei, 430068, China; Corresponding author. Membrane Protein Disease Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, T6G 2H7, Edmonton, AB, Canada.Transient receptor potential (TRP) polycystin-3 (TRPP3) is a non-selective cation channel activated by Ca2+ and protons and is involved in regulating ciliary Ca2+ concentration, hedgehog signaling and sour tasting. The TRPP3 channel function and regulation are still not well understood. Here we investigated regulation of TRPP3 by calmodulin (CaM) by means of electrophysiology and Xenopus oocytes as an expression model. We found that TRPP3 channel function is enhanced by calmidazolium, a CaM antagonist, and inhibited by CaM through binding of the CaM N-lobe to a TRPP3 C-terminal domain not overlapped with the EF-hand. We further revealed that the TRPP3/CaM interaction promotes phosphorylation of TRPP3 at threonine 591 by Ca2+/CaM-dependent protein kinase II, which mediates the inhibition of TRPP3 by CaM.http://www.sciencedirect.com/science/article/pii/S2772892723000123TRP channelCaMCalciumCaMK2ElectrophysiologyXenopus oocyte |
| spellingShingle | Xiong Liu Yifang Wang Ziyi Weng Qinyi Xu Cefan Zhou JingFeng Tang Xing-Zhen Chen Inhibition of TRPP3 by calmodulin through Ca2+/calmodulin-dependent protein kinase II Cell Insight TRP channel CaM Calcium CaMK2 Electrophysiology Xenopus oocyte |
| title | Inhibition of TRPP3 by calmodulin through Ca2+/calmodulin-dependent protein kinase II |
| title_full | Inhibition of TRPP3 by calmodulin through Ca2+/calmodulin-dependent protein kinase II |
| title_fullStr | Inhibition of TRPP3 by calmodulin through Ca2+/calmodulin-dependent protein kinase II |
| title_full_unstemmed | Inhibition of TRPP3 by calmodulin through Ca2+/calmodulin-dependent protein kinase II |
| title_short | Inhibition of TRPP3 by calmodulin through Ca2+/calmodulin-dependent protein kinase II |
| title_sort | inhibition of trpp3 by calmodulin through ca2 calmodulin dependent protein kinase ii |
| topic | TRP channel CaM Calcium CaMK2 Electrophysiology Xenopus oocyte |
| url | http://www.sciencedirect.com/science/article/pii/S2772892723000123 |
| work_keys_str_mv | AT xiongliu inhibitionoftrpp3bycalmodulinthroughca2calmodulindependentproteinkinaseii AT yifangwang inhibitionoftrpp3bycalmodulinthroughca2calmodulindependentproteinkinaseii AT ziyiweng inhibitionoftrpp3bycalmodulinthroughca2calmodulindependentproteinkinaseii AT qinyixu inhibitionoftrpp3bycalmodulinthroughca2calmodulindependentproteinkinaseii AT cefanzhou inhibitionoftrpp3bycalmodulinthroughca2calmodulindependentproteinkinaseii AT jingfengtang inhibitionoftrpp3bycalmodulinthroughca2calmodulindependentproteinkinaseii AT xingzhenchen inhibitionoftrpp3bycalmodulinthroughca2calmodulindependentproteinkinaseii |