Performance of Different Cotton and Nylon Swabs on DNA Recovery and Storage
Touch DNA samples are routine yet challenging pieces of evidence that provide investigators with information that helps them solve crimes. However, this type of evidence can be easily lost if the correct collection method is not used. This problem could be overcome with an optimal method of collecti...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Arabic |
| Published: |
Naif University Publishing House
2023-09-01
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| Series: | Arab Journal of Forensic Sciences & Forensic Medicine |
| Subjects: | |
| Online Access: | https://journals.nauss.edu.sa/index.php/AJFSFM/article/view/2242 |
| _version_ | 1827320085553872896 |
|---|---|
| author | Mohammed Alrahma Hanan Almulla Suaad Alshehhi Maryam Almuhairi Naima Aljanahi Ayesha Alsabhan Hussain Alghanim |
| author_facet | Mohammed Alrahma Hanan Almulla Suaad Alshehhi Maryam Almuhairi Naima Aljanahi Ayesha Alsabhan Hussain Alghanim |
| author_sort | Mohammed Alrahma |
| collection | DOAJ |
| description | Touch DNA samples are routine yet challenging pieces of evidence that provide investigators with information that helps them solve crimes. However, this type of evidence can be easily lost if the correct collection method is not used. This problem could be overcome with an optimal method of collection that increases the amount of touch DNA collected from different types of surfaces. Better-quality touch DNA can increase the chances of getting a full genetic profile. This study was divided into two parts which aimed to assess whether the type of swab used on different surfaces will significantly increase DNA recovery, concentrations, and the DNA preservation during three different timeframes (24h, 1 month and 3 months). Two different cotton swabs and Nylon swabs were used to lift touch DNA on three different surfaces (glass, plastic and wood) to identify the most suitable method of collection across all three surfaces. A total of 72 samples were lifted (3 replicates from each swab on 3 different surfaces) from two different participants (Male and Female) which were left to dry for 14 days in room temperature prior to DNA extraction. DNA preservation of the swabs was observed while using three dilutions of blood sample which was prepared from one of the volunteers (1:1 – 1:10 – 1:20) where 10 uL of each dilution was pipetted onto the four types of swabs in three replicates (n=36) to observe the preservation over three different timeframes 24h storage, 1 Month and 3 Months with a total of 108 samples. The COPAN CLASSIQSwabsTM Dry swab showed an overall average result during the storage periods of 24h with (1:1) dilution by (2.694ng/μL), (1:10) dilution with (0.548ng/μL) and (1:20) dilution with (0.143ng/μL). Results for the period of 1 Month also showed an average of (1:1) dilution with (2.825ng/μL), (1:10) dilution with (0.361ng/μL) and (1:20) dilution with (0.156ng/μL). These findings can be helpful for laboratories and crime scene investigators to optimize DNA sample collection and preservation based on their workflow. |
| first_indexed | 2024-04-25T00:39:16Z |
| format | Article |
| id | doaj.art-00ccc831ae58476b9f867bbded8eb8cd |
| institution | Directory Open Access Journal |
| issn | 1658-6786 1658-6794 |
| language | Arabic |
| last_indexed | 2024-04-25T00:39:16Z |
| publishDate | 2023-09-01 |
| publisher | Naif University Publishing House |
| record_format | Article |
| series | Arab Journal of Forensic Sciences & Forensic Medicine |
| spelling | doaj.art-00ccc831ae58476b9f867bbded8eb8cd2024-03-12T12:27:57ZaraNaif University Publishing HouseArab Journal of Forensic Sciences & Forensic Medicine1658-67861658-67942023-09-015213514310.26735/YQMY91901946Performance of Different Cotton and Nylon Swabs on DNA Recovery and StorageMohammed Alrahma0https://orcid.org/0000-0001-5128-7935Hanan Almulla1Suaad Alshehhi2Maryam Almuhairi3Naima Aljanahi4Ayesha Alsabhan5Hussain Alghanim6General Department of Forensic Science and Criminology, Dubai Police, United Arab Emirates.General Department of Forensic Science and Criminology, Dubai Police, United Arab Emirates.General Department of Forensic Science and Criminology, Dubai Police, United Arab Emirates.General Department of Forensic Science and Criminology, Dubai Police, United Arab Emirates.General Department of Forensic Science and Criminology, Dubai Police, United Arab Emirates.General Department of Forensic Science and Criminology, Dubai Police, United Arab Emirates.General Department of Forensic Science and Criminology, Dubai Police, United Arab Emirates.Touch DNA samples are routine yet challenging pieces of evidence that provide investigators with information that helps them solve crimes. However, this type of evidence can be easily lost if the correct collection method is not used. This problem could be overcome with an optimal method of collection that increases the amount of touch DNA collected from different types of surfaces. Better-quality touch DNA can increase the chances of getting a full genetic profile. This study was divided into two parts which aimed to assess whether the type of swab used on different surfaces will significantly increase DNA recovery, concentrations, and the DNA preservation during three different timeframes (24h, 1 month and 3 months). Two different cotton swabs and Nylon swabs were used to lift touch DNA on three different surfaces (glass, plastic and wood) to identify the most suitable method of collection across all three surfaces. A total of 72 samples were lifted (3 replicates from each swab on 3 different surfaces) from two different participants (Male and Female) which were left to dry for 14 days in room temperature prior to DNA extraction. DNA preservation of the swabs was observed while using three dilutions of blood sample which was prepared from one of the volunteers (1:1 – 1:10 – 1:20) where 10 uL of each dilution was pipetted onto the four types of swabs in three replicates (n=36) to observe the preservation over three different timeframes 24h storage, 1 Month and 3 Months with a total of 108 samples. The COPAN CLASSIQSwabsTM Dry swab showed an overall average result during the storage periods of 24h with (1:1) dilution by (2.694ng/μL), (1:10) dilution with (0.548ng/μL) and (1:20) dilution with (0.143ng/μL). Results for the period of 1 Month also showed an average of (1:1) dilution with (2.825ng/μL), (1:10) dilution with (0.361ng/μL) and (1:20) dilution with (0.156ng/μL). These findings can be helpful for laboratories and crime scene investigators to optimize DNA sample collection and preservation based on their workflow.https://journals.nauss.edu.sa/index.php/AJFSFM/article/view/2242forensic sciencedna preservationtouch dnatrace dnadna collection |
| spellingShingle | Mohammed Alrahma Hanan Almulla Suaad Alshehhi Maryam Almuhairi Naima Aljanahi Ayesha Alsabhan Hussain Alghanim Performance of Different Cotton and Nylon Swabs on DNA Recovery and Storage Arab Journal of Forensic Sciences & Forensic Medicine forensic science dna preservation touch dna trace dna dna collection |
| title | Performance of Different Cotton and Nylon Swabs on DNA Recovery and Storage |
| title_full | Performance of Different Cotton and Nylon Swabs on DNA Recovery and Storage |
| title_fullStr | Performance of Different Cotton and Nylon Swabs on DNA Recovery and Storage |
| title_full_unstemmed | Performance of Different Cotton and Nylon Swabs on DNA Recovery and Storage |
| title_short | Performance of Different Cotton and Nylon Swabs on DNA Recovery and Storage |
| title_sort | performance of different cotton and nylon swabs on dna recovery and storage |
| topic | forensic science dna preservation touch dna trace dna dna collection |
| url | https://journals.nauss.edu.sa/index.php/AJFSFM/article/view/2242 |
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