EVALUATION OF ENDOMETRIAL BIOPSIES FOR CHRONIC ENDOMETRITIS BY USING SYNDECAN-1 (CD138) IN ABNORMAL UTERINE BLEEDING
Objective: To determine the utility of Syndecan–1 (CD138) in the diagnosis of Chronic endometritis in patients with Abnormal Uterine Bleeding. Study Design: Cross-sectional study. Place and Duration: The study was carried out in department of Pathology, Army Medical College, Rawalpindi in coll...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Army Medical College Rawalpindi
2019-12-01
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| Series: | Pakistan Armed Forces Medical Journal |
| Subjects: | |
| Online Access: | https://www.pafmj.org/index.php/PAFMJ/article/view/3662/2483 |
| _version_ | 1831718741152366592 |
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| author | Aaminah Hanif Tariq Sarfraz Humaira Tariq Saleem Ahmed Khan Syed Raza Jaffar Farhan Akhtar |
| author_facet | Aaminah Hanif Tariq Sarfraz Humaira Tariq Saleem Ahmed Khan Syed Raza Jaffar Farhan Akhtar |
| author_sort | Aaminah Hanif |
| collection | DOAJ |
| description | Objective: To determine the utility of Syndecan–1 (CD138) in the diagnosis of Chronic endometritis in patients with Abnormal Uterine Bleeding.
Study Design: Cross-sectional study.
Place and Duration: The study was carried out in department of Pathology, Army Medical College, Rawalpindi in collaboration with the department of Obstetrics & Gynecology, Pak Emirates Military Hospital (PEMH), Rawalpindi. The study duration was from Jan 2018 to Oct 2018.
Methodology: A total of one hundred endometrial biopsies of patients with clinical or histological suspicion of chronic endometritis were taken. Hematoxylin and Eosin (H & E) stain was performed on all the biopsies and a diagnosis was made. Later on, Syndecan-1 was applied on all the biopsies and an immunohistochemical diagnosis was made. Both the diagnoses were then compared and analyzed.
Results: Mean age of the study participants was 43.67 ± 8.95 years, with a range of 23-70 years. Out of 100 biopsies, forty-five cases (45%) were diagnosed as Chronic endometritis on H & E, while fifty-five cases (55%) were not showing Chronic endometritis and were diagnosed as disordered proliferative endometrium (DPE). On application of Syndecan-1 (CD 138), it was seen that these 19 out of 45 cases, diagnosed as Chronic endometritis on H & E were actually over-diagnosed and didn’t show plasma cells. Out of 55 cases which were negative for Chronic endometritis, 15 cases were underdiagnosed as they showed plasma cells with immunohistochemistry application (Syndecan-1).
Conclusion: In cases suspected as Chronic endometritis, no plasma cells were clearly visible on H & E staining, Sydnecan-1 helps identifying plasma cells easily. |
| first_indexed | 2024-12-21T01:22:00Z |
| format | Article |
| id | doaj.art-00cdb95dd2904f3b97fe2e7fef31c138 |
| institution | Directory Open Access Journal |
| issn | 0030-9648 2411-8842 |
| language | English |
| last_indexed | 2024-12-21T01:22:00Z |
| publishDate | 2019-12-01 |
| publisher | Army Medical College Rawalpindi |
| record_format | Article |
| series | Pakistan Armed Forces Medical Journal |
| spelling | doaj.art-00cdb95dd2904f3b97fe2e7fef31c1382022-12-21T19:20:37ZengArmy Medical College RawalpindiPakistan Armed Forces Medical Journal0030-96482411-88422019-12-0169613411345EVALUATION OF ENDOMETRIAL BIOPSIES FOR CHRONIC ENDOMETRITIS BY USING SYNDECAN-1 (CD138) IN ABNORMAL UTERINE BLEEDINGAaminah Hanif0Tariq Sarfraz1Humaira Tariq2Saleem Ahmed Khan3Syed Raza Jaffar4Farhan Akhtar5Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi PakistanArmy Medical College/National University of Medical Sciences (NUMS) Rawalpindi PakistanPak Emirates Military Hospital/National University of Medical Sciences (NUMS) Rawalpindi PakistanArmy Medical College/National University of Medical Sciences (NUMS) Rawalpindi PakistanArmy Medical College/National University of Medical Sciences (NUMS) Rawalpindi PakistanArmy Medical College/National University of Medical Sciences (NUMS) Rawalpindi PakistanObjective: To determine the utility of Syndecan–1 (CD138) in the diagnosis of Chronic endometritis in patients with Abnormal Uterine Bleeding. Study Design: Cross-sectional study. Place and Duration: The study was carried out in department of Pathology, Army Medical College, Rawalpindi in collaboration with the department of Obstetrics & Gynecology, Pak Emirates Military Hospital (PEMH), Rawalpindi. The study duration was from Jan 2018 to Oct 2018. Methodology: A total of one hundred endometrial biopsies of patients with clinical or histological suspicion of chronic endometritis were taken. Hematoxylin and Eosin (H & E) stain was performed on all the biopsies and a diagnosis was made. Later on, Syndecan-1 was applied on all the biopsies and an immunohistochemical diagnosis was made. Both the diagnoses were then compared and analyzed. Results: Mean age of the study participants was 43.67 ± 8.95 years, with a range of 23-70 years. Out of 100 biopsies, forty-five cases (45%) were diagnosed as Chronic endometritis on H & E, while fifty-five cases (55%) were not showing Chronic endometritis and were diagnosed as disordered proliferative endometrium (DPE). On application of Syndecan-1 (CD 138), it was seen that these 19 out of 45 cases, diagnosed as Chronic endometritis on H & E were actually over-diagnosed and didn’t show plasma cells. Out of 55 cases which were negative for Chronic endometritis, 15 cases were underdiagnosed as they showed plasma cells with immunohistochemistry application (Syndecan-1). Conclusion: In cases suspected as Chronic endometritis, no plasma cells were clearly visible on H & E staining, Sydnecan-1 helps identifying plasma cells easily.https://www.pafmj.org/index.php/PAFMJ/article/view/3662/2483abnormal uterine bleedingchronic endometritisplasma cell |
| spellingShingle | Aaminah Hanif Tariq Sarfraz Humaira Tariq Saleem Ahmed Khan Syed Raza Jaffar Farhan Akhtar EVALUATION OF ENDOMETRIAL BIOPSIES FOR CHRONIC ENDOMETRITIS BY USING SYNDECAN-1 (CD138) IN ABNORMAL UTERINE BLEEDING Pakistan Armed Forces Medical Journal abnormal uterine bleeding chronic endometritis plasma cell |
| title | EVALUATION OF ENDOMETRIAL BIOPSIES FOR CHRONIC ENDOMETRITIS BY USING SYNDECAN-1 (CD138) IN ABNORMAL UTERINE BLEEDING |
| title_full | EVALUATION OF ENDOMETRIAL BIOPSIES FOR CHRONIC ENDOMETRITIS BY USING SYNDECAN-1 (CD138) IN ABNORMAL UTERINE BLEEDING |
| title_fullStr | EVALUATION OF ENDOMETRIAL BIOPSIES FOR CHRONIC ENDOMETRITIS BY USING SYNDECAN-1 (CD138) IN ABNORMAL UTERINE BLEEDING |
| title_full_unstemmed | EVALUATION OF ENDOMETRIAL BIOPSIES FOR CHRONIC ENDOMETRITIS BY USING SYNDECAN-1 (CD138) IN ABNORMAL UTERINE BLEEDING |
| title_short | EVALUATION OF ENDOMETRIAL BIOPSIES FOR CHRONIC ENDOMETRITIS BY USING SYNDECAN-1 (CD138) IN ABNORMAL UTERINE BLEEDING |
| title_sort | evaluation of endometrial biopsies for chronic endometritis by using syndecan 1 cd138 in abnormal uterine bleeding |
| topic | abnormal uterine bleeding chronic endometritis plasma cell |
| url | https://www.pafmj.org/index.php/PAFMJ/article/view/3662/2483 |
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