Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus
Background: Ethanol has been pointed out as a laccase inducer. However, there are controversial reports about its efficiency with some fungi. In this study, we hypothesized that ethanol laccase induced in Pycnoporus sanguineus depends on nitrogen nutriment conditions. To prove this, we assessed lacc...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-07-01
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| Series: | Electronic Journal of Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345815000731 |
| _version_ | 1819081036184682496 |
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| author | Christian A. Hernández Norma Sandoval Julieta Mallerman José Antonio García-Pérez Anne-Marie Farnet Isabelle Perraud-Gaime Enrique Alarcón |
| author_facet | Christian A. Hernández Norma Sandoval Julieta Mallerman José Antonio García-Pérez Anne-Marie Farnet Isabelle Perraud-Gaime Enrique Alarcón |
| author_sort | Christian A. Hernández |
| collection | DOAJ |
| description | Background: Ethanol has been pointed out as a laccase inducer. However, there are controversial reports about its efficiency with some fungi. In this study, we hypothesized that ethanol laccase induced in Pycnoporus sanguineus depends on nitrogen nutriment conditions. To prove this, we assessed laccase production in submerged cultures of P. sanguineus, with different nitrogen concentrations and with, or without ethanol added in a factorial designed experiment.
Results: In order to analyze the effects of factors on the response variables, a factorial ANOVA, and response-surface models were performed. It was found that the nitrogen source was the main factor that affected laccase production in P. sanguineus. The treatments with yeast extract (2 g/L) and ethanol (3 g/L) induced the highest laccase activity (31.01 ± 4.9 U/L), while the treatments with urea reached the lowest activity (less than 1.6 U/L). Ethanol had positive and synergic effects on laccase production, in accordance with the surface response model, as long as simple nitrogen sources (urea) were not available.
Conclusions: We suggest that laccase in P. sanguineus is regulated by a catabolic nitrogen repression mechanism; laccase activity is strongly inhibited by urea used as nitrogen source and it decreases when the amount of urea increases; contrarily, a synergic positive effect was observed between yeast extract and ethanol on laccase production. |
| first_indexed | 2024-12-21T19:54:23Z |
| format | Article |
| id | doaj.art-00cdd4720c9c48b493f859b0fff70783 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-21T19:54:23Z |
| publishDate | 2015-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-00cdd4720c9c48b493f859b0fff707832022-12-21T18:52:07ZengElsevierElectronic Journal of Biotechnology0717-34582015-07-0118432733210.1016/j.ejbt.2015.05.008Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineusChristian A. Hernández0Norma Sandoval1Julieta Mallerman2José Antonio García-Pérez3Anne-Marie Farnet4Isabelle Perraud-Gaime5Enrique Alarcón6Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana, Xalapa, Veracruz, MexicoInstituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana, Xalapa, Veracruz, MexicoUniversidad de Buenos Aires (UBA), Buenos Aires, ArgentinaFacultad de Biología, Universidad Veracruzana, Xalapa, Veracruz, MexicoEquipe Systèmes Microbiens IMBE, UMR CNRS 7263, IRD 237, Faculté des Sciences Techniques de St. Jérôme, Aix Marseille Université, Marseille, FranceEquipe Ecotechnologies et Bioremediation, IMBE, UMR CNRS 7263, IRD 237, Faculté des Sciences et Techniques de St. Jérôme, Aix Marseille Université, Marseille, FranceInstituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana, Xalapa, Veracruz, MexicoBackground: Ethanol has been pointed out as a laccase inducer. However, there are controversial reports about its efficiency with some fungi. In this study, we hypothesized that ethanol laccase induced in Pycnoporus sanguineus depends on nitrogen nutriment conditions. To prove this, we assessed laccase production in submerged cultures of P. sanguineus, with different nitrogen concentrations and with, or without ethanol added in a factorial designed experiment. Results: In order to analyze the effects of factors on the response variables, a factorial ANOVA, and response-surface models were performed. It was found that the nitrogen source was the main factor that affected laccase production in P. sanguineus. The treatments with yeast extract (2 g/L) and ethanol (3 g/L) induced the highest laccase activity (31.01 ± 4.9 U/L), while the treatments with urea reached the lowest activity (less than 1.6 U/L). Ethanol had positive and synergic effects on laccase production, in accordance with the surface response model, as long as simple nitrogen sources (urea) were not available. Conclusions: We suggest that laccase in P. sanguineus is regulated by a catabolic nitrogen repression mechanism; laccase activity is strongly inhibited by urea used as nitrogen source and it decreases when the amount of urea increases; contrarily, a synergic positive effect was observed between yeast extract and ethanol on laccase production.http://www.sciencedirect.com/science/article/pii/S0717345815000731Enzyme productionFungal metabolismPhenoloxidasesUreaYeast extract |
| spellingShingle | Christian A. Hernández Norma Sandoval Julieta Mallerman José Antonio García-Pérez Anne-Marie Farnet Isabelle Perraud-Gaime Enrique Alarcón Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus Electronic Journal of Biotechnology Enzyme production Fungal metabolism Phenoloxidases Urea Yeast extract |
| title | Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus |
| title_full | Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus |
| title_fullStr | Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus |
| title_full_unstemmed | Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus |
| title_short | Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus |
| title_sort | ethanol induction of laccase depends on nitrogen conditions of pycnoporus sanguineus |
| topic | Enzyme production Fungal metabolism Phenoloxidases Urea Yeast extract |
| url | http://www.sciencedirect.com/science/article/pii/S0717345815000731 |
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