Dysregulated proinflammatory and fibrogenic phenotype of fibroblasts in cystic fibrosis.
Morbi-mortality in cystic fibrosis (CF) is mainly related to chronic lung infection and inflammation, uncontrolled tissue rearrangements and fibrosis, and yet the underlying mechanisms remain largely unknown. We evaluated inflammatory and fibrosis responses to bleomycin in F508del homozygous and wil...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3667188?pdf=render |
| _version_ | 1818509837803192320 |
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| author | François Huaux Sabrina Noel Barbara Dhooghe Nadtha Panin Sandra Lo Re Dominique Lison Pierre Wallemacq Etienne Marbaix Bob J Scholte Patrick Lebecque Teresinha Leal |
| author_facet | François Huaux Sabrina Noel Barbara Dhooghe Nadtha Panin Sandra Lo Re Dominique Lison Pierre Wallemacq Etienne Marbaix Bob J Scholte Patrick Lebecque Teresinha Leal |
| author_sort | François Huaux |
| collection | DOAJ |
| description | Morbi-mortality in cystic fibrosis (CF) is mainly related to chronic lung infection and inflammation, uncontrolled tissue rearrangements and fibrosis, and yet the underlying mechanisms remain largely unknown. We evaluated inflammatory and fibrosis responses to bleomycin in F508del homozygous and wild-type mice, and phenotype of fibroblasts explanted from mouse lungs and skin. The effect of vardenafil, a cGMP-specific phosphodiesterase type 5 inhibitor, was tested in vivo and in culture. Responses of proinflammatory and fibrotic markers to bleomycin were enhanced in lungs and skin of CF mice and were prevented by treatment with vardenafil. Purified lung and skin fibroblasts from CF mice proliferated and differentiated into myofibroblasts more prominently and displayed higher sensitivity to growth factors than those recovered from wild-type littermates. Under inflammatory stimulation, mRNA and protein expression of proinflammatory mediators were higher in CF than in wild-type fibroblasts, in which CFTR expression reached similar levels to those observed in other non-epithelial cells, such as macrophages. Increased proinflammatory responses in CF fibroblasts were reduced by half with submicromolar concentrations of vardenafil. Proinflammatory and fibrogenic functions of fibroblasts are upregulated in CF and are reduced by vardenafil. This study provides compelling new support for targeting cGMP signaling pathway in CF pharmacotherapy. |
| first_indexed | 2024-12-10T22:50:54Z |
| format | Article |
| id | doaj.art-00ce6e4f1e3e4fa98df65436e39cc34b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T22:50:54Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-00ce6e4f1e3e4fa98df65436e39cc34b2022-12-22T01:30:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6434110.1371/journal.pone.0064341Dysregulated proinflammatory and fibrogenic phenotype of fibroblasts in cystic fibrosis.François HuauxSabrina NoelBarbara DhoogheNadtha PaninSandra Lo ReDominique LisonPierre WallemacqEtienne MarbaixBob J ScholtePatrick LebecqueTeresinha LealMorbi-mortality in cystic fibrosis (CF) is mainly related to chronic lung infection and inflammation, uncontrolled tissue rearrangements and fibrosis, and yet the underlying mechanisms remain largely unknown. We evaluated inflammatory and fibrosis responses to bleomycin in F508del homozygous and wild-type mice, and phenotype of fibroblasts explanted from mouse lungs and skin. The effect of vardenafil, a cGMP-specific phosphodiesterase type 5 inhibitor, was tested in vivo and in culture. Responses of proinflammatory and fibrotic markers to bleomycin were enhanced in lungs and skin of CF mice and were prevented by treatment with vardenafil. Purified lung and skin fibroblasts from CF mice proliferated and differentiated into myofibroblasts more prominently and displayed higher sensitivity to growth factors than those recovered from wild-type littermates. Under inflammatory stimulation, mRNA and protein expression of proinflammatory mediators were higher in CF than in wild-type fibroblasts, in which CFTR expression reached similar levels to those observed in other non-epithelial cells, such as macrophages. Increased proinflammatory responses in CF fibroblasts were reduced by half with submicromolar concentrations of vardenafil. Proinflammatory and fibrogenic functions of fibroblasts are upregulated in CF and are reduced by vardenafil. This study provides compelling new support for targeting cGMP signaling pathway in CF pharmacotherapy.http://europepmc.org/articles/PMC3667188?pdf=render |
| spellingShingle | François Huaux Sabrina Noel Barbara Dhooghe Nadtha Panin Sandra Lo Re Dominique Lison Pierre Wallemacq Etienne Marbaix Bob J Scholte Patrick Lebecque Teresinha Leal Dysregulated proinflammatory and fibrogenic phenotype of fibroblasts in cystic fibrosis. PLoS ONE |
| title | Dysregulated proinflammatory and fibrogenic phenotype of fibroblasts in cystic fibrosis. |
| title_full | Dysregulated proinflammatory and fibrogenic phenotype of fibroblasts in cystic fibrosis. |
| title_fullStr | Dysregulated proinflammatory and fibrogenic phenotype of fibroblasts in cystic fibrosis. |
| title_full_unstemmed | Dysregulated proinflammatory and fibrogenic phenotype of fibroblasts in cystic fibrosis. |
| title_short | Dysregulated proinflammatory and fibrogenic phenotype of fibroblasts in cystic fibrosis. |
| title_sort | dysregulated proinflammatory and fibrogenic phenotype of fibroblasts in cystic fibrosis |
| url | http://europepmc.org/articles/PMC3667188?pdf=render |
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