Are <i>pvcrt-o</i> and <i>pvmdr1</i> Gene Mutations Associated with <i>Plasmodium vivax</i> Chloroquine-Resistant Parasites?
(1) Background: Malaria remains a significant global public health issue. Since parasites quickly became resistant to most of the available antimalarial drugs, treatment effectiveness must be constantly monitored. In Brazil, up to 10% of cases of vivax malaria resistant to chloroquine (CQ) have been...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/12/1/141 |
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| author | Rebecca de Abreu-Fernandes Natália Ketrin Almeida-de-Oliveira Aline Rosa de Lavigne Mello Lucas Tavares de Queiroz Jacqueline de Aguiar Barros Bárbara de Oliveira Baptista Joseli Oliveira-Ferreira Rodrigo Medeiros de Souza Lilian Rose Pratt-Riccio Patrícia Brasil Cláudio Tadeu Daniel-Ribeiro Maria de Fátima Ferreira-da-Cruz |
| author_facet | Rebecca de Abreu-Fernandes Natália Ketrin Almeida-de-Oliveira Aline Rosa de Lavigne Mello Lucas Tavares de Queiroz Jacqueline de Aguiar Barros Bárbara de Oliveira Baptista Joseli Oliveira-Ferreira Rodrigo Medeiros de Souza Lilian Rose Pratt-Riccio Patrícia Brasil Cláudio Tadeu Daniel-Ribeiro Maria de Fátima Ferreira-da-Cruz |
| author_sort | Rebecca de Abreu-Fernandes |
| collection | DOAJ |
| description | (1) Background: Malaria remains a significant global public health issue. Since parasites quickly became resistant to most of the available antimalarial drugs, treatment effectiveness must be constantly monitored. In Brazil, up to 10% of cases of vivax malaria resistant to chloroquine (CQ) have been registered. Unlike <i>P. falciparum</i>, there are no definitive molecular markers for the chemoresistance of <i>P. vivax</i> to CQ. This work aimed to investigate whether polymorphisms in the <i>pvcrt-o</i> and <i>pvmdr1</i> genes could be used as markers for assessing its resistance to CQ. (2) Methods: A total of 130 samples from <i>P. vivax</i> malaria cases with no clinical and/or parasitological evidence of CQ resistance were studied through polymerase chain reaction for gene amplification followed by target DNA sequencing. (3) Results: In the <i>pvcrt-o</i> exons, the K10 insert was present in 14% of the isolates. Regarding <i>pvmdr1</i>, T958<b>M</b> and F1076<b>L</b> haplotypes showed frequencies of 95% and 3%, respectively, while the SNP Y976<b>F</b> was not detected. (4) Conclusions: Since K10-<i>pvcrt-o</i> and F1076<b>L</b>/T958<b>M</b>-<i>pvmdr1</i> polymorphisms were detected in samples from patients who responded well to CQ treatment, it can be concluded that mutations in these genes do not seem to have a potential for association with the phenotype of CQ resistance. |
| first_indexed | 2024-03-08T09:56:26Z |
| format | Article |
| id | doaj.art-00d04e7f46b1466ea4c6f17c4ff8e31a |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-08T09:56:26Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-00d04e7f46b1466ea4c6f17c4ff8e31a2024-01-29T13:47:29ZengMDPI AGBiomedicines2227-90592024-01-0112114110.3390/biomedicines12010141Are <i>pvcrt-o</i> and <i>pvmdr1</i> Gene Mutations Associated with <i>Plasmodium vivax</i> Chloroquine-Resistant Parasites?Rebecca de Abreu-Fernandes0Natália Ketrin Almeida-de-Oliveira1Aline Rosa de Lavigne Mello2Lucas Tavares de Queiroz3Jacqueline de Aguiar Barros4Bárbara de Oliveira Baptista5Joseli Oliveira-Ferreira6Rodrigo Medeiros de Souza7Lilian Rose Pratt-Riccio8Patrícia Brasil9Cláudio Tadeu Daniel-Ribeiro10Maria de Fátima Ferreira-da-Cruz11Laboratório de Pesquisa em Malária, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21041-361, BrazilLaboratório de Pesquisa em Malária, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21041-361, BrazilLaboratório de Pesquisa em Malária, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21041-361, BrazilLaboratório de Pesquisa em Malária, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21041-361, BrazilLaboratório de Pesquisa em Malária, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21041-361, BrazilLaboratório de Pesquisa em Malária, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21041-361, BrazilLaboratório de Imunoparasitologia, IOC, Fiocruz, Rio de Janeiro 21041-361, BrazilLaboratório de Doenças Infecciosas da Amazônia Ocidental, Universidade Federal do Acre, Campus Floresta, Cruzeiro do Sul 69980-000, BrazilLaboratório de Pesquisa em Malária, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21041-361, BrazilCentro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal), Reference Laboratory for Malaria in the Extra-Amazonian Region for the Brazilian Ministry of Health, Secretaria de Vigilância Sanitária & Fiocruz, Rio de Janeiro 21041-361, BrazilLaboratório de Pesquisa em Malária, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21041-361, BrazilLaboratório de Pesquisa em Malária, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21041-361, Brazil(1) Background: Malaria remains a significant global public health issue. Since parasites quickly became resistant to most of the available antimalarial drugs, treatment effectiveness must be constantly monitored. In Brazil, up to 10% of cases of vivax malaria resistant to chloroquine (CQ) have been registered. Unlike <i>P. falciparum</i>, there are no definitive molecular markers for the chemoresistance of <i>P. vivax</i> to CQ. This work aimed to investigate whether polymorphisms in the <i>pvcrt-o</i> and <i>pvmdr1</i> genes could be used as markers for assessing its resistance to CQ. (2) Methods: A total of 130 samples from <i>P. vivax</i> malaria cases with no clinical and/or parasitological evidence of CQ resistance were studied through polymerase chain reaction for gene amplification followed by target DNA sequencing. (3) Results: In the <i>pvcrt-o</i> exons, the K10 insert was present in 14% of the isolates. Regarding <i>pvmdr1</i>, T958<b>M</b> and F1076<b>L</b> haplotypes showed frequencies of 95% and 3%, respectively, while the SNP Y976<b>F</b> was not detected. (4) Conclusions: Since K10-<i>pvcrt-o</i> and F1076<b>L</b>/T958<b>M</b>-<i>pvmdr1</i> polymorphisms were detected in samples from patients who responded well to CQ treatment, it can be concluded that mutations in these genes do not seem to have a potential for association with the phenotype of CQ resistance.https://www.mdpi.com/2227-9059/12/1/141chloroquinechemoresistancemalaria<i>P. vivax</i><i>pvcrt-o</i><i>pvmdr1</i> |
| spellingShingle | Rebecca de Abreu-Fernandes Natália Ketrin Almeida-de-Oliveira Aline Rosa de Lavigne Mello Lucas Tavares de Queiroz Jacqueline de Aguiar Barros Bárbara de Oliveira Baptista Joseli Oliveira-Ferreira Rodrigo Medeiros de Souza Lilian Rose Pratt-Riccio Patrícia Brasil Cláudio Tadeu Daniel-Ribeiro Maria de Fátima Ferreira-da-Cruz Are <i>pvcrt-o</i> and <i>pvmdr1</i> Gene Mutations Associated with <i>Plasmodium vivax</i> Chloroquine-Resistant Parasites? Biomedicines chloroquine chemoresistance malaria <i>P. vivax</i> <i>pvcrt-o</i> <i>pvmdr1</i> |
| title | Are <i>pvcrt-o</i> and <i>pvmdr1</i> Gene Mutations Associated with <i>Plasmodium vivax</i> Chloroquine-Resistant Parasites? |
| title_full | Are <i>pvcrt-o</i> and <i>pvmdr1</i> Gene Mutations Associated with <i>Plasmodium vivax</i> Chloroquine-Resistant Parasites? |
| title_fullStr | Are <i>pvcrt-o</i> and <i>pvmdr1</i> Gene Mutations Associated with <i>Plasmodium vivax</i> Chloroquine-Resistant Parasites? |
| title_full_unstemmed | Are <i>pvcrt-o</i> and <i>pvmdr1</i> Gene Mutations Associated with <i>Plasmodium vivax</i> Chloroquine-Resistant Parasites? |
| title_short | Are <i>pvcrt-o</i> and <i>pvmdr1</i> Gene Mutations Associated with <i>Plasmodium vivax</i> Chloroquine-Resistant Parasites? |
| title_sort | are i pvcrt o i and i pvmdr1 i gene mutations associated with i plasmodium vivax i chloroquine resistant parasites |
| topic | chloroquine chemoresistance malaria <i>P. vivax</i> <i>pvcrt-o</i> <i>pvmdr1</i> |
| url | https://www.mdpi.com/2227-9059/12/1/141 |
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