Intersex goats show different gene expression levels in the hypothalamus and pituitary compared with non‐intersex goats based on RNA‐Seq
Abstract The conditions for sex reversal in vertebrate species have been extensively studied, and the results highlighted numerous key factors involved in sex differentiation. However, the transcriptomes in hypothalamic and pituitary tissues from intersex goats have rarely been studied. The aim of t...
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| Format: | Article |
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Wiley
2022-01-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.672 |
| _version_ | 1798025128557150208 |
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| author | Haoyuan Han Shuai Yang Jun Li Jinyan Zhao Hongfang Wei Si Ha Wantao Li Congcong Li Kai Quan |
| author_facet | Haoyuan Han Shuai Yang Jun Li Jinyan Zhao Hongfang Wei Si Ha Wantao Li Congcong Li Kai Quan |
| author_sort | Haoyuan Han |
| collection | DOAJ |
| description | Abstract The conditions for sex reversal in vertebrate species have been extensively studied, and the results highlighted numerous key factors involved in sex differentiation. However, the transcriptomes in hypothalamic and pituitary tissues from intersex goats have rarely been studied. The aim of this study was to screen candidate genes and signalling pathways related to sex reversal in Huai goats by analyzing gene expression in hypothalamic and pituitary tissues via transcriptome sequencing and bioinformatics analyses. In total, 612 and 139 differentially expressed genes (DEGs) were identified between the intersex and non‐intersex groups in the hypothalamus and pituitary, respectively. The DEGs in the hypothalamus and pituitary were significantly enriched in 41 and 16 signalling pathways, respectively, including the calcium signalling pathway, neuroactive ligand‐receptor interaction signalling pathway, and oestrogen signalling pathway, which might be related to intersex sex development disorders. A candidate gene from the tachykinin family (TACR1) was significantly enriched in the calcium signalling pathway. Thirty‐one DEGs were shared between these two comparisons and were enriched in several acetyl‐CoA‐related processes and the oestrogen signalling pathway. The results of the real‐time PCR analysis show that the transcriptome sequencing results were reliable. The transcriptome data indicate that the regulation of various physiological systems is involved in intersex goat development. Therefore, these results provide helpful data enhancing our understanding of the molecular mechanisms underlying intersex syndrome in goats. |
| first_indexed | 2024-04-11T18:13:43Z |
| format | Article |
| id | doaj.art-00d06bc8d2404da485c1e7e9a668a778 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-04-11T18:13:43Z |
| publishDate | 2022-01-01 |
| publisher | Wiley |
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| series | Veterinary Medicine and Science |
| spelling | doaj.art-00d06bc8d2404da485c1e7e9a668a7782022-12-22T04:09:59ZengWileyVeterinary Medicine and Science2053-10952022-01-018136737610.1002/vms3.672Intersex goats show different gene expression levels in the hypothalamus and pituitary compared with non‐intersex goats based on RNA‐SeqHaoyuan Han0Shuai Yang1Jun Li2Jinyan Zhao3Hongfang Wei4Si Ha5Wantao Li6Congcong Li7Kai Quan8College of Animal Science and Technology Henan University of Animal Husbandry and Economy Zhengzhou P. R. ChinaCollege of Veterinary Medicine Gansu Agricultural University Lanzhou P. R. ChinaCollege of Animal Science and Technology Henan University of Animal Husbandry and Economy Zhengzhou P. R. ChinaCollege of Animal Science and Technology Henan University of Animal Husbandry and Economy Zhengzhou P. R. ChinaCollege of Animal Science and Technology Henan University of Animal Husbandry and Economy Zhengzhou P. R. ChinaCollege of Animal Science and Technology Henan University of Animal Husbandry and Economy Zhengzhou P. R. ChinaHenan Genetic Protection Engineering Research Center for Livestock and Poultry Zhengzhou Henan P. R. ChinaCollege of Animal Science and Technology Henan University of Animal Husbandry and Economy Zhengzhou P. R. ChinaCollege of Animal Science and Technology Henan University of Animal Husbandry and Economy Zhengzhou P. R. ChinaAbstract The conditions for sex reversal in vertebrate species have been extensively studied, and the results highlighted numerous key factors involved in sex differentiation. However, the transcriptomes in hypothalamic and pituitary tissues from intersex goats have rarely been studied. The aim of this study was to screen candidate genes and signalling pathways related to sex reversal in Huai goats by analyzing gene expression in hypothalamic and pituitary tissues via transcriptome sequencing and bioinformatics analyses. In total, 612 and 139 differentially expressed genes (DEGs) were identified between the intersex and non‐intersex groups in the hypothalamus and pituitary, respectively. The DEGs in the hypothalamus and pituitary were significantly enriched in 41 and 16 signalling pathways, respectively, including the calcium signalling pathway, neuroactive ligand‐receptor interaction signalling pathway, and oestrogen signalling pathway, which might be related to intersex sex development disorders. A candidate gene from the tachykinin family (TACR1) was significantly enriched in the calcium signalling pathway. Thirty‐one DEGs were shared between these two comparisons and were enriched in several acetyl‐CoA‐related processes and the oestrogen signalling pathway. The results of the real‐time PCR analysis show that the transcriptome sequencing results were reliable. The transcriptome data indicate that the regulation of various physiological systems is involved in intersex goat development. Therefore, these results provide helpful data enhancing our understanding of the molecular mechanisms underlying intersex syndrome in goats.https://doi.org/10.1002/vms3.672differentially expressed genesHuai goatintersexualitytranscriptome |
| spellingShingle | Haoyuan Han Shuai Yang Jun Li Jinyan Zhao Hongfang Wei Si Ha Wantao Li Congcong Li Kai Quan Intersex goats show different gene expression levels in the hypothalamus and pituitary compared with non‐intersex goats based on RNA‐Seq Veterinary Medicine and Science differentially expressed genes Huai goat intersexuality transcriptome |
| title | Intersex goats show different gene expression levels in the hypothalamus and pituitary compared with non‐intersex goats based on RNA‐Seq |
| title_full | Intersex goats show different gene expression levels in the hypothalamus and pituitary compared with non‐intersex goats based on RNA‐Seq |
| title_fullStr | Intersex goats show different gene expression levels in the hypothalamus and pituitary compared with non‐intersex goats based on RNA‐Seq |
| title_full_unstemmed | Intersex goats show different gene expression levels in the hypothalamus and pituitary compared with non‐intersex goats based on RNA‐Seq |
| title_short | Intersex goats show different gene expression levels in the hypothalamus and pituitary compared with non‐intersex goats based on RNA‐Seq |
| title_sort | intersex goats show different gene expression levels in the hypothalamus and pituitary compared with non intersex goats based on rna seq |
| topic | differentially expressed genes Huai goat intersexuality transcriptome |
| url | https://doi.org/10.1002/vms3.672 |
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