Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection
Thai rice cultivar RD6 is well known for its cooking and eating qualities. However it is susceptible to blast disease, a major rice disease caused by the fungus Magnaporthe oryzae. This study focused on the pyramiding of four QTLs for blast resistance located on chromosomes 1, 2, 11 and 12, from two...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2017-03-01
|
| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-201701-0002_pyramiding-of-four-blast-resistance-qtls-into-thai-rice-cultivar-rd6-through-marker-assisted-selection.php |
| _version_ | 1797899379066011648 |
|---|---|
| author | Teerawat SUWANNUAL Sompong CHANKAEW Tidarat MONKHAM Weerasak SAKSIRIRAT Jirawat SANITCHON |
| author_facet | Teerawat SUWANNUAL Sompong CHANKAEW Tidarat MONKHAM Weerasak SAKSIRIRAT Jirawat SANITCHON |
| author_sort | Teerawat SUWANNUAL |
| collection | DOAJ |
| description | Thai rice cultivar RD6 is well known for its cooking and eating qualities. However it is susceptible to blast disease, a major rice disease caused by the fungus Magnaporthe oryzae. This study focused on the pyramiding of four QTLs for blast resistance located on chromosomes 1, 2, 11 and 12, from two RD6 introgression lines. Marker-assisted selection was performed and facilitated the selection with 8 microsatellite flanking markers to enable the selection in BC2F2:3 lines. All possible combinations of the four QTL alleles were assessed for blast resistance by artificial inoculation using 8 diverse isolates in a greenhouse and under field conditions using the upland short row method. The results showed that the RD6 introgression lines carrying a high number of QTLs for blast resitance achieved from pyramiding have high levels of blast resistance and broad spectrum of resistance to the blast pathogens prevalent in the region. Only one of the M. oryzae isolates, THL185, was virulent to all the breeding lines, suggesting that the identification of new blast resistance genes or QTLs and pyramiding them into RD6 for durable blast resistance and no yield penalty should be the focus of further research. |
| first_indexed | 2024-04-10T08:29:00Z |
| format | Article |
| id | doaj.art-00d2f86ca00641e8a69558e6db7fc635 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:29:00Z |
| publishDate | 2017-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-00d2f86ca00641e8a69558e6db7fc6352023-02-23T03:30:38ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252017-03-015311810.17221/51/2016-CJGPBcjg-201701-0002Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selectionTeerawat SUWANNUAL0Sompong CHANKAEW1Tidarat MONKHAM2Weerasak SAKSIRIRAT3Jirawat SANITCHONDepartment of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen, ThailandDepartment of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen, ThailandDepartment of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen, ThailandDepartment of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen, ThailandThai rice cultivar RD6 is well known for its cooking and eating qualities. However it is susceptible to blast disease, a major rice disease caused by the fungus Magnaporthe oryzae. This study focused on the pyramiding of four QTLs for blast resistance located on chromosomes 1, 2, 11 and 12, from two RD6 introgression lines. Marker-assisted selection was performed and facilitated the selection with 8 microsatellite flanking markers to enable the selection in BC2F2:3 lines. All possible combinations of the four QTL alleles were assessed for blast resistance by artificial inoculation using 8 diverse isolates in a greenhouse and under field conditions using the upland short row method. The results showed that the RD6 introgression lines carrying a high number of QTLs for blast resitance achieved from pyramiding have high levels of blast resistance and broad spectrum of resistance to the blast pathogens prevalent in the region. Only one of the M. oryzae isolates, THL185, was virulent to all the breeding lines, suggesting that the identification of new blast resistance genes or QTLs and pyramiding them into RD6 for durable blast resistance and no yield penalty should be the focus of further research.https://cjgpb.agriculturejournals.cz/artkey/cjg-201701-0002_pyramiding-of-four-blast-resistance-qtls-into-thai-rice-cultivar-rd6-through-marker-assisted-selection.phpbroad spectrumintrogression linesmagnaporthe oryzaeoryza sativaseverity index |
| spellingShingle | Teerawat SUWANNUAL Sompong CHANKAEW Tidarat MONKHAM Weerasak SAKSIRIRAT Jirawat SANITCHON Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection Czech Journal of Genetics and Plant Breeding broad spectrum introgression lines magnaporthe oryzae oryza sativa severity index |
| title | Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection |
| title_full | Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection |
| title_fullStr | Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection |
| title_full_unstemmed | Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection |
| title_short | Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection |
| title_sort | pyramiding of four blast resistance qtls into thai rice cultivar rd6 through marker assisted selection |
| topic | broad spectrum introgression lines magnaporthe oryzae oryza sativa severity index |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-201701-0002_pyramiding-of-four-blast-resistance-qtls-into-thai-rice-cultivar-rd6-through-marker-assisted-selection.php |
| work_keys_str_mv | AT teerawatsuwannual pyramidingoffourblastresistanceqtlsintothairicecultivarrd6throughmarkerassistedselection AT sompongchankaew pyramidingoffourblastresistanceqtlsintothairicecultivarrd6throughmarkerassistedselection AT tidaratmonkham pyramidingoffourblastresistanceqtlsintothairicecultivarrd6throughmarkerassistedselection AT weerasaksaksirirat pyramidingoffourblastresistanceqtlsintothairicecultivarrd6throughmarkerassistedselection AT jirawatsanitchon pyramidingoffourblastresistanceqtlsintothairicecultivarrd6throughmarkerassistedselection |