Effect of Sema6D on Proliferation, Migration, Invasion and Angiogenesis-promoting Ability of Human Osteosarcoma Cells and Its Mechanism
Objective To investigate the effect of Sema6D knockdown on the proliferation, migration, invasion and angiogenesis-promoting ability of human osteosarcoma cell lines. Methods The expression of Sema6D in clinical tissues and cell lines of human osteosarcoma was detected. After the targeted siRNA tran...
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| Format: | Article |
| Language: | zho |
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Magazine House of Cancer Research on Prevention and Treatment
2022-04-01
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| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2022.21.0957 |
| _version_ | 1817991185300455424 |
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| author | LIU Yixin XU Tianzi NING Biao LEI Jun WEI Yongchang |
| author_facet | LIU Yixin XU Tianzi NING Biao LEI Jun WEI Yongchang |
| author_sort | LIU Yixin |
| collection | DOAJ |
| description | Objective To investigate the effect of Sema6D knockdown on the proliferation, migration, invasion and angiogenesis-promoting ability of human osteosarcoma cell lines. Methods The expression of Sema6D in clinical tissues and cell lines of human osteosarcoma was detected. After the targeted siRNA transfection, the changes of proliferation, migration and invasion were measured by CCK-8, wound healing and Transwell experiments. HUVECs were co-cultured with tumor conditioned medium to detect their tube formation ability. And the expression of signal pathway proteins was detected by Western blot. Results Sema6D was highly expressed in human osteosarcoma tissues and cell lines(P < 0.05). After silencing Sema6D, the proliferation, migration and invasion of 143B and MG63 cells decreased significantly(P < 0.05), the angiogenesis ability of HUVECs decreased in vitro(P < 0.01), and the expression of PI3K/AKT/mTOR and ERK-related signal pathway proteins decreased(P < 0.05). Conclusion Sema6D is overexpressed in human osteosarcoma tissues and cell lines. Knockdown of Sema6D expression level could inhibit the proliferation, migration, invasion and angiogenesis-promoting ability of human osteosarcoma cells via reducing PI3K/AKT/mTOR and ERK signal pathway. |
| first_indexed | 2024-04-14T01:09:52Z |
| format | Article |
| id | doaj.art-00d6ef07112b4322be3ff9563b0be293 |
| institution | Directory Open Access Journal |
| issn | 1000-8578 |
| language | zho |
| last_indexed | 2024-04-14T01:09:52Z |
| publishDate | 2022-04-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-00d6ef07112b4322be3ff9563b0be2932022-12-22T02:21:07ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85782022-04-0149431432110.3971/j.issn.1000-8578.2022.21.09578578.2022.21.0957Effect of Sema6D on Proliferation, Migration, Invasion and Angiogenesis-promoting Ability of Human Osteosarcoma Cells and Its MechanismLIU Yixin0XU Tianzi1NING Biao2LEI Jun3WEI Yongchang4Department of Radiotherapy and Chemotherapy of Oncology, Zhongnan Hospital of Wuhan University, Wuhan 430071, ChinaDepartment of Radiotherapy and Chemotherapy of Oncology, Zhongnan Hospital of Wuhan University, Wuhan 430071, ChinaDepartment of Radiotherapy and Chemotherapy of Oncology, Zhongnan Hospital of Wuhan University, Wuhan 430071, ChinaDepartment of Orthopaedics, Zhongnan Hospital of Wuhan University, Wuhan 430071, ChinaDepartment of Radiotherapy and Chemotherapy of Oncology, Zhongnan Hospital of Wuhan University, Wuhan 430071, ChinaObjective To investigate the effect of Sema6D knockdown on the proliferation, migration, invasion and angiogenesis-promoting ability of human osteosarcoma cell lines. Methods The expression of Sema6D in clinical tissues and cell lines of human osteosarcoma was detected. After the targeted siRNA transfection, the changes of proliferation, migration and invasion were measured by CCK-8, wound healing and Transwell experiments. HUVECs were co-cultured with tumor conditioned medium to detect their tube formation ability. And the expression of signal pathway proteins was detected by Western blot. Results Sema6D was highly expressed in human osteosarcoma tissues and cell lines(P < 0.05). After silencing Sema6D, the proliferation, migration and invasion of 143B and MG63 cells decreased significantly(P < 0.05), the angiogenesis ability of HUVECs decreased in vitro(P < 0.01), and the expression of PI3K/AKT/mTOR and ERK-related signal pathway proteins decreased(P < 0.05). Conclusion Sema6D is overexpressed in human osteosarcoma tissues and cell lines. Knockdown of Sema6D expression level could inhibit the proliferation, migration, invasion and angiogenesis-promoting ability of human osteosarcoma cells via reducing PI3K/AKT/mTOR and ERK signal pathway.http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2022.21.0957osteosarcomasema6drna interferenceproliferationmigrationinvasion |
| spellingShingle | LIU Yixin XU Tianzi NING Biao LEI Jun WEI Yongchang Effect of Sema6D on Proliferation, Migration, Invasion and Angiogenesis-promoting Ability of Human Osteosarcoma Cells and Its Mechanism Zhongliu Fangzhi Yanjiu osteosarcoma sema6d rna interference proliferation migration invasion |
| title | Effect of Sema6D on Proliferation, Migration, Invasion and Angiogenesis-promoting Ability of Human Osteosarcoma Cells and Its Mechanism |
| title_full | Effect of Sema6D on Proliferation, Migration, Invasion and Angiogenesis-promoting Ability of Human Osteosarcoma Cells and Its Mechanism |
| title_fullStr | Effect of Sema6D on Proliferation, Migration, Invasion and Angiogenesis-promoting Ability of Human Osteosarcoma Cells and Its Mechanism |
| title_full_unstemmed | Effect of Sema6D on Proliferation, Migration, Invasion and Angiogenesis-promoting Ability of Human Osteosarcoma Cells and Its Mechanism |
| title_short | Effect of Sema6D on Proliferation, Migration, Invasion and Angiogenesis-promoting Ability of Human Osteosarcoma Cells and Its Mechanism |
| title_sort | effect of sema6d on proliferation migration invasion and angiogenesis promoting ability of human osteosarcoma cells and its mechanism |
| topic | osteosarcoma sema6d rna interference proliferation migration invasion |
| url | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2022.21.0957 |
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