DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars

Rice blast is the most important fungal disease of rice and is caused by Pyricularia oryzae Sacc. (Telomorph Magnoporthe grisea Barr.). Seven randomly amplified polymorphic DNA (RAPD) markers OPA5, OPG17, OPG18, OPG19, OPF9, OPF17 and OPF19 showed very clear polymorphism in resistant cultivar lines...

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Main Authors: S.S. Sandhu, Carlos Colombo, Cândido R. Bastos, Walter J. Siqueira
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2003-12-01
Series:Genetics and Molecular Biology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400011
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author S.S. Sandhu
Carlos Colombo
Cândido R. Bastos
Walter J. Siqueira
author_facet S.S. Sandhu
Carlos Colombo
Cândido R. Bastos
Walter J. Siqueira
author_sort S.S. Sandhu
collection DOAJ
description Rice blast is the most important fungal disease of rice and is caused by Pyricularia oryzae Sacc. (Telomorph Magnoporthe grisea Barr.). Seven randomly amplified polymorphic DNA (RAPD) markers OPA5, OPG17, OPG18, OPG19, OPF9, OPF17 and OPF19 showed very clear polymorphism in resistant cultivar lines which differed from susceptible lines. By comparing different susceptible lines, nine DNA amplifications of seven primers (OPA5(1000), OPA5(1200,) OPG17(700), OPG18(850), OPG19(500), OPG19(600), OPF9(600), OPF17(1200) and OPF19(600)) were identified as dominant markers for the blast resistant gene in resistant cultivar lines. These loci facilitate the indirect scoring of blast resistant and blast susceptible genotypes. The codomine RAPDs markers will facilitate marker-assisted selection of the blast resistant gene in two blast resistant genotypes of rice (Labelle and Line 11) and will be useful in rice breeding programs.
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spelling doaj.art-00d84c5007e24d638a2ce058d3210adb2022-12-22T00:57:37ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852003-12-0126447347610.1590/S1415-47572003000400011DNA tagging of blast resistant gene(s) in three Brazilian rice cultivarsS.S. SandhuCarlos ColomboCândido R. BastosWalter J. SiqueiraRice blast is the most important fungal disease of rice and is caused by Pyricularia oryzae Sacc. (Telomorph Magnoporthe grisea Barr.). Seven randomly amplified polymorphic DNA (RAPD) markers OPA5, OPG17, OPG18, OPG19, OPF9, OPF17 and OPF19 showed very clear polymorphism in resistant cultivar lines which differed from susceptible lines. By comparing different susceptible lines, nine DNA amplifications of seven primers (OPA5(1000), OPA5(1200,) OPG17(700), OPG18(850), OPG19(500), OPG19(600), OPF9(600), OPF17(1200) and OPF19(600)) were identified as dominant markers for the blast resistant gene in resistant cultivar lines. These loci facilitate the indirect scoring of blast resistant and blast susceptible genotypes. The codomine RAPDs markers will facilitate marker-assisted selection of the blast resistant gene in two blast resistant genotypes of rice (Labelle and Line 11) and will be useful in rice breeding programs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400011riceSCARarbitrary primersPyricularia oryzae
spellingShingle S.S. Sandhu
Carlos Colombo
Cândido R. Bastos
Walter J. Siqueira
DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars
Genetics and Molecular Biology
rice
SCAR
arbitrary primers
Pyricularia oryzae
title DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars
title_full DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars
title_fullStr DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars
title_full_unstemmed DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars
title_short DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars
title_sort dna tagging of blast resistant gene s in three brazilian rice cultivars
topic rice
SCAR
arbitrary primers
Pyricularia oryzae
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400011
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