DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars
Rice blast is the most important fungal disease of rice and is caused by Pyricularia oryzae Sacc. (Telomorph Magnoporthe grisea Barr.). Seven randomly amplified polymorphic DNA (RAPD) markers OPA5, OPG17, OPG18, OPG19, OPF9, OPF17 and OPF19 showed very clear polymorphism in resistant cultivar lines...
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Language: | English |
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Sociedade Brasileira de Genética
2003-12-01
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Series: | Genetics and Molecular Biology |
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400011 |
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author | S.S. Sandhu Carlos Colombo Cândido R. Bastos Walter J. Siqueira |
author_facet | S.S. Sandhu Carlos Colombo Cândido R. Bastos Walter J. Siqueira |
author_sort | S.S. Sandhu |
collection | DOAJ |
description | Rice blast is the most important fungal disease of rice and is caused by Pyricularia oryzae Sacc. (Telomorph Magnoporthe grisea Barr.). Seven randomly amplified polymorphic DNA (RAPD) markers OPA5, OPG17, OPG18, OPG19, OPF9, OPF17 and OPF19 showed very clear polymorphism in resistant cultivar lines which differed from susceptible lines. By comparing different susceptible lines, nine DNA amplifications of seven primers (OPA5(1000), OPA5(1200,) OPG17(700), OPG18(850), OPG19(500), OPG19(600), OPF9(600), OPF17(1200) and OPF19(600)) were identified as dominant markers for the blast resistant gene in resistant cultivar lines. These loci facilitate the indirect scoring of blast resistant and blast susceptible genotypes. The codomine RAPDs markers will facilitate marker-assisted selection of the blast resistant gene in two blast resistant genotypes of rice (Labelle and Line 11) and will be useful in rice breeding programs. |
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institution | Directory Open Access Journal |
issn | 1415-4757 1678-4685 |
language | English |
last_indexed | 2024-12-11T17:07:51Z |
publishDate | 2003-12-01 |
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series | Genetics and Molecular Biology |
spelling | doaj.art-00d84c5007e24d638a2ce058d3210adb2022-12-22T00:57:37ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852003-12-0126447347610.1590/S1415-47572003000400011DNA tagging of blast resistant gene(s) in three Brazilian rice cultivarsS.S. SandhuCarlos ColomboCândido R. BastosWalter J. SiqueiraRice blast is the most important fungal disease of rice and is caused by Pyricularia oryzae Sacc. (Telomorph Magnoporthe grisea Barr.). Seven randomly amplified polymorphic DNA (RAPD) markers OPA5, OPG17, OPG18, OPG19, OPF9, OPF17 and OPF19 showed very clear polymorphism in resistant cultivar lines which differed from susceptible lines. By comparing different susceptible lines, nine DNA amplifications of seven primers (OPA5(1000), OPA5(1200,) OPG17(700), OPG18(850), OPG19(500), OPG19(600), OPF9(600), OPF17(1200) and OPF19(600)) were identified as dominant markers for the blast resistant gene in resistant cultivar lines. These loci facilitate the indirect scoring of blast resistant and blast susceptible genotypes. The codomine RAPDs markers will facilitate marker-assisted selection of the blast resistant gene in two blast resistant genotypes of rice (Labelle and Line 11) and will be useful in rice breeding programs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400011riceSCARarbitrary primersPyricularia oryzae |
spellingShingle | S.S. Sandhu Carlos Colombo Cândido R. Bastos Walter J. Siqueira DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars Genetics and Molecular Biology rice SCAR arbitrary primers Pyricularia oryzae |
title | DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars |
title_full | DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars |
title_fullStr | DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars |
title_full_unstemmed | DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars |
title_short | DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars |
title_sort | dna tagging of blast resistant gene s in three brazilian rice cultivars |
topic | rice SCAR arbitrary primers Pyricularia oryzae |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400011 |
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