Determination of Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MAC) of seed methanolic extract of Peganumharmala L. against Gram negative bacteria
Peganum harmala L. (Zygophyllaceae) used in traditional medicine of Iraq and itis one of the most famous medicinal plants. The most bacteria pathogen is resistance for synthesis antibiotic drugs resulting from abuse uses of the patient or produce resistance strains for microorganism therefor, the...
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, University of Kufa
2018-06-01
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| Series: | Kufa Journal for Veterinary Medical Sciences |
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| Online Access: | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4097 |
| _version_ | 1797364054171320320 |
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| author | Saadia Saleh Mehdy Al-zeiny |
| author_facet | Saadia Saleh Mehdy Al-zeiny |
| author_sort | Saadia Saleh Mehdy Al-zeiny |
| collection | DOAJ |
| description |
Peganum harmala L. (Zygophyllaceae) used in traditional medicine of Iraq and itis one of the most famous medicinal plants. The most bacteria pathogen is resistance for synthesis antibiotic drugs resulting from abuse uses of the patient or produce resistance strains for microorganism therefor, the aim of this search is the effect antibacterial of seed alcoholic extract of peganum harmala on some Gram negative bacteria pathogen. Methods: The broth serial dilution assay method determined the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of seed methanolic extract of Peganum harmala were tested for antibacterial effect against bacterial isolates (salmonlla enteric , klebsiela pneumonia multidrugresitant E. Coli and highly resist E.coli), used phytochemical analysis of seed mathanolic extrac of peganumharmala. Result: showed good antibacterial activity against strain Salmonella enteric and Klebsella pneumonia, were MBC at concentration (25 mg/ml) and no effect of low concentration of MIC. The MBC value against strain multidrugresistant Escherichia coli, was at concentration (3.125 mg/ml) and MIC at (25 and 12.5 mg/ml), while MBC of highly resist Escherichia coli was in (12.5mg/ml) and MIC value at 50 mg/ml compared with standard antibiotics synthesis. Phytochemical analysis of component seed methanolic extract of peganumharmala showed presence of flavonoids, alkaloids, saponins, tannins, glycosides, terpenoids and steroids.
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| first_indexed | 2024-03-08T16:29:50Z |
| format | Article |
| id | doaj.art-00daa48b5cf74faba2516d50fd3f3856 |
| institution | Directory Open Access Journal |
| issn | 2077-9798 2959-8478 |
| language | English |
| last_indexed | 2024-03-08T16:29:50Z |
| publishDate | 2018-06-01 |
| publisher | Faculty of Veterinary Medicine, University of Kufa |
| record_format | Article |
| series | Kufa Journal for Veterinary Medical Sciences |
| spelling | doaj.art-00daa48b5cf74faba2516d50fd3f38562024-01-06T10:13:14ZengFaculty of Veterinary Medicine, University of KufaKufa Journal for Veterinary Medical Sciences2077-97982959-84782018-06-019110.36326/kjvs/2018/v9i14097Determination of Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MAC) of seed methanolic extract of Peganumharmala L. against Gram negative bacteriaSaadia Saleh Mehdy Al-zeiny Peganum harmala L. (Zygophyllaceae) used in traditional medicine of Iraq and itis one of the most famous medicinal plants. The most bacteria pathogen is resistance for synthesis antibiotic drugs resulting from abuse uses of the patient or produce resistance strains for microorganism therefor, the aim of this search is the effect antibacterial of seed alcoholic extract of peganum harmala on some Gram negative bacteria pathogen. Methods: The broth serial dilution assay method determined the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of seed methanolic extract of Peganum harmala were tested for antibacterial effect against bacterial isolates (salmonlla enteric , klebsiela pneumonia multidrugresitant E. Coli and highly resist E.coli), used phytochemical analysis of seed mathanolic extrac of peganumharmala. Result: showed good antibacterial activity against strain Salmonella enteric and Klebsella pneumonia, were MBC at concentration (25 mg/ml) and no effect of low concentration of MIC. The MBC value against strain multidrugresistant Escherichia coli, was at concentration (3.125 mg/ml) and MIC at (25 and 12.5 mg/ml), while MBC of highly resist Escherichia coli was in (12.5mg/ml) and MIC value at 50 mg/ml compared with standard antibiotics synthesis. Phytochemical analysis of component seed methanolic extract of peganumharmala showed presence of flavonoids, alkaloids, saponins, tannins, glycosides, terpenoids and steroids. https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4097Antibacterial activitySeed methanolic extractPeganum harmala |
| spellingShingle | Saadia Saleh Mehdy Al-zeiny Determination of Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MAC) of seed methanolic extract of Peganumharmala L. against Gram negative bacteria Kufa Journal for Veterinary Medical Sciences Antibacterial activity Seed methanolic extract Peganum harmala |
| title | Determination of Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MAC) of seed methanolic extract of Peganumharmala L. against Gram negative bacteria |
| title_full | Determination of Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MAC) of seed methanolic extract of Peganumharmala L. against Gram negative bacteria |
| title_fullStr | Determination of Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MAC) of seed methanolic extract of Peganumharmala L. against Gram negative bacteria |
| title_full_unstemmed | Determination of Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MAC) of seed methanolic extract of Peganumharmala L. against Gram negative bacteria |
| title_short | Determination of Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MAC) of seed methanolic extract of Peganumharmala L. against Gram negative bacteria |
| title_sort | determination of minimum inhibitory concentration mic and minimum bactericidal concentration mac of seed methanolic extract of peganumharmala l against gram negative bacteria |
| topic | Antibacterial activity Seed methanolic extract Peganum harmala |
| url | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4097 |
| work_keys_str_mv | AT saadiasalehmehdyalzeiny determinationofminimuminhibitoryconcentrationmicandminimumbactericidalconcentrationmacofseedmethanolicextractofpeganumharmalalagainstgramnegativebacteria |