16S rRNA-based detection of oral pathogens in coronary atherosclerotic plaque
<b>Background:</b> Atherosclerosis develops as a response of the vessel wall to injury. Chronic bacterial infections have been associated with an increased risk for atherosclerosis and coronary artery disease. The ability of oral pathogens to colonize in coronary atheromatous plaque is w...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2010-01-01
|
| Series: | Indian Journal of Dental Research |
| Subjects: | |
| Online Access: | http://www.ijdr.in/article.asp?issn=0970-9290;year=2010;volume=21;issue=2;spage=248;epage=252;aulast=Mahendra |
| _version_ | 1819087556123295744 |
|---|---|
| author | Mahendra Jaideep Mahendra Little Kurian V Jaishankar K Mythilli R |
| author_facet | Mahendra Jaideep Mahendra Little Kurian V Jaishankar K Mythilli R |
| author_sort | Mahendra Jaideep |
| collection | DOAJ |
| description | <b>Background:</b> Atherosclerosis develops as a response of the vessel wall to injury. Chronic bacterial infections have been associated with an increased risk for atherosclerosis and coronary artery disease. The ability of oral pathogens to colonize in coronary atheromatous plaque is well known. <b>Aim:</b> The aim of this study was to detect the presence of <i>Treponema denticola</i>, <i>Porphyromonas gingivalis and Campylobacter rectus</i> in the subgingival and atherosclerotic plaques of patients with coronary artery disease. <b>Materials and Methods:</b> Fifty-one patients in the age group of 40-80 years with coronary artery disease were selected for the study. DNA was extracted from the plaque samples. The specific primers for <i>T. denticola</i>, <i>C. rectus and P. gingivalis</i> were used to amplify a part of the 16S rRNA gene by polymerase chain reaction. <b>Statistical Analysis Used:</b> Chi-square analysis, correlation coefficient and prevalence percentage of the microorganisms were carried out for the analysis. <b>Results:</b> Of the 51 patients, <i>T. denticola</i>, <i>C. rectus and P. gingivalis</i> were detected in 49.01%, 21.51% and 45.10% of the atherosclerotic plaque samples. <b>Conclusions:</b> Our study revealed the presence of bacterial DNA of the oral pathogenic microorganisms in coronary atherosclerotic plaques. The presence of the bacterial DNA in the coronary atherosclerotic plaques in significant proportion may suggest the possible relationship between periodontal bacterial infection and genesis of coronary atherosclerosis. |
| first_indexed | 2024-12-21T21:38:01Z |
| format | Article |
| id | doaj.art-00db18d67f8f48c1a8e4d19b7d9faf7d |
| institution | Directory Open Access Journal |
| issn | 0970-9290 1998-3603 |
| language | English |
| last_indexed | 2024-12-21T21:38:01Z |
| publishDate | 2010-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Dental Research |
| spelling | doaj.art-00db18d67f8f48c1a8e4d19b7d9faf7d2022-12-21T18:49:25ZengWolters Kluwer Medknow PublicationsIndian Journal of Dental Research0970-92901998-36032010-01-0121224825216S rRNA-based detection of oral pathogens in coronary atherosclerotic plaqueMahendra JaideepMahendra LittleKurian VJaishankar KMythilli R<b>Background:</b> Atherosclerosis develops as a response of the vessel wall to injury. Chronic bacterial infections have been associated with an increased risk for atherosclerosis and coronary artery disease. The ability of oral pathogens to colonize in coronary atheromatous plaque is well known. <b>Aim:</b> The aim of this study was to detect the presence of <i>Treponema denticola</i>, <i>Porphyromonas gingivalis and Campylobacter rectus</i> in the subgingival and atherosclerotic plaques of patients with coronary artery disease. <b>Materials and Methods:</b> Fifty-one patients in the age group of 40-80 years with coronary artery disease were selected for the study. DNA was extracted from the plaque samples. The specific primers for <i>T. denticola</i>, <i>C. rectus and P. gingivalis</i> were used to amplify a part of the 16S rRNA gene by polymerase chain reaction. <b>Statistical Analysis Used:</b> Chi-square analysis, correlation coefficient and prevalence percentage of the microorganisms were carried out for the analysis. <b>Results:</b> Of the 51 patients, <i>T. denticola</i>, <i>C. rectus and P. gingivalis</i> were detected in 49.01%, 21.51% and 45.10% of the atherosclerotic plaque samples. <b>Conclusions:</b> Our study revealed the presence of bacterial DNA of the oral pathogenic microorganisms in coronary atherosclerotic plaques. The presence of the bacterial DNA in the coronary atherosclerotic plaques in significant proportion may suggest the possible relationship between periodontal bacterial infection and genesis of coronary atherosclerosis.http://www.ijdr.in/article.asp?issn=0970-9290;year=2010;volume=21;issue=2;spage=248;epage=252;aulast=MahendraAtherosclerosisperiodontal diseasesperiodontal pathogens5 polymerase chain reaction |
| spellingShingle | Mahendra Jaideep Mahendra Little Kurian V Jaishankar K Mythilli R 16S rRNA-based detection of oral pathogens in coronary atherosclerotic plaque Indian Journal of Dental Research Atherosclerosis periodontal diseases periodontal pathogens 5 polymerase chain reaction |
| title | 16S rRNA-based detection of oral pathogens in coronary atherosclerotic plaque |
| title_full | 16S rRNA-based detection of oral pathogens in coronary atherosclerotic plaque |
| title_fullStr | 16S rRNA-based detection of oral pathogens in coronary atherosclerotic plaque |
| title_full_unstemmed | 16S rRNA-based detection of oral pathogens in coronary atherosclerotic plaque |
| title_short | 16S rRNA-based detection of oral pathogens in coronary atherosclerotic plaque |
| title_sort | 16s rrna based detection of oral pathogens in coronary atherosclerotic plaque |
| topic | Atherosclerosis periodontal diseases periodontal pathogens 5 polymerase chain reaction |
| url | http://www.ijdr.in/article.asp?issn=0970-9290;year=2010;volume=21;issue=2;spage=248;epage=252;aulast=Mahendra |
| work_keys_str_mv | AT mahendrajaideep 16srrnabaseddetectionoforalpathogensincoronaryatheroscleroticplaque AT mahendralittle 16srrnabaseddetectionoforalpathogensincoronaryatheroscleroticplaque AT kurianv 16srrnabaseddetectionoforalpathogensincoronaryatheroscleroticplaque AT jaishankark 16srrnabaseddetectionoforalpathogensincoronaryatheroscleroticplaque AT mythillir 16srrnabaseddetectionoforalpathogensincoronaryatheroscleroticplaque |