Effects of Manure and Chemical Fertilizer on Bacterial Community Structure and Soil Enzyme Activities in North China
The application of organic fertilizer affects soil microbes and enzyme activities. In this study, we explored the effects of various long-term different fertilization treatments (manure, M; chemical fertilizer, NP; manure + chemical fertilizer, MNP; and no fertilizer, CK) on bacterial community stru...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/5/1017 |
| _version_ | 1797533248940670976 |
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| author | Zhiping Liu Wenyan Xie Zhenxing Yang Xuefang Huang Huaiping Zhou |
| author_facet | Zhiping Liu Wenyan Xie Zhenxing Yang Xuefang Huang Huaiping Zhou |
| author_sort | Zhiping Liu |
| collection | DOAJ |
| description | The application of organic fertilizer affects soil microbes and enzyme activities. In this study, we explored the effects of various long-term different fertilization treatments (manure, M; chemical fertilizer, NP; manure + chemical fertilizer, MNP; and no fertilizer, CK) on bacterial community structure and soil sucrase, urease, and alkaline phosphatase activities in Shaping, Hequ, China. High-throughput sequencing was used to amplify the third to the fourth hypervariable region of the 16S ribosomal RNA for analysis of the bacterial community structure. Enzyme activities were determined by colorimetry. Soil treated with MNP had the highest bacterial Abundance-based Coverage Estimator index and enzyme activities. The principal coordinates analysis results showed significant differences among the various fertilization treatments (<i>p</i> < 0.001). Proteobacteria, Actinobacteria, Acidobacteria, Gemmatimonadetes, and Chloroflexi were consistently dominant in all soil samples. The redundancy analysis and Monte Carlo permutation tests showed that the soil bacterial communities were significantly correlated with alkali-hydrolyzable nitrogen, organic matter, urease, and alkaline phosphatase. Our results reveal the fundamentally different effects that organic and inorganic fertilizers have on soil bacterial communities and their functions. |
| first_indexed | 2024-03-10T11:11:51Z |
| format | Article |
| id | doaj.art-00dc00e3993d472b93f1de4123e62f5a |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T11:11:51Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-00dc00e3993d472b93f1de4123e62f5a2023-11-21T20:40:02ZengMDPI AGAgronomy2073-43952021-05-01115101710.3390/agronomy11051017Effects of Manure and Chemical Fertilizer on Bacterial Community Structure and Soil Enzyme Activities in North ChinaZhiping Liu0Wenyan Xie1Zhenxing Yang2Xuefang Huang3Huaiping Zhou4College of Resources and Environment, Shanxi Agricultural University, Taiyuan 030031, ChinaCollege of Resources and Environment, Shanxi Agricultural University, Taiyuan 030031, ChinaCollege of Resources and Environment, Shanxi Agricultural University, Taiyuan 030031, ChinaShanxi Institute of Organic Dryland Farming, Shanxi Agricultural University, Taiyuan 030031, ChinaCollege of Resources and Environment, Shanxi Agricultural University, Taiyuan 030031, ChinaThe application of organic fertilizer affects soil microbes and enzyme activities. In this study, we explored the effects of various long-term different fertilization treatments (manure, M; chemical fertilizer, NP; manure + chemical fertilizer, MNP; and no fertilizer, CK) on bacterial community structure and soil sucrase, urease, and alkaline phosphatase activities in Shaping, Hequ, China. High-throughput sequencing was used to amplify the third to the fourth hypervariable region of the 16S ribosomal RNA for analysis of the bacterial community structure. Enzyme activities were determined by colorimetry. Soil treated with MNP had the highest bacterial Abundance-based Coverage Estimator index and enzyme activities. The principal coordinates analysis results showed significant differences among the various fertilization treatments (<i>p</i> < 0.001). Proteobacteria, Actinobacteria, Acidobacteria, Gemmatimonadetes, and Chloroflexi were consistently dominant in all soil samples. The redundancy analysis and Monte Carlo permutation tests showed that the soil bacterial communities were significantly correlated with alkali-hydrolyzable nitrogen, organic matter, urease, and alkaline phosphatase. Our results reveal the fundamentally different effects that organic and inorganic fertilizers have on soil bacterial communities and their functions.https://www.mdpi.com/2073-4395/11/5/1017bacterial communitylong-term fertilizationsoil enzyme activitieshigh-throughput sequencing |
| spellingShingle | Zhiping Liu Wenyan Xie Zhenxing Yang Xuefang Huang Huaiping Zhou Effects of Manure and Chemical Fertilizer on Bacterial Community Structure and Soil Enzyme Activities in North China Agronomy bacterial community long-term fertilization soil enzyme activities high-throughput sequencing |
| title | Effects of Manure and Chemical Fertilizer on Bacterial Community Structure and Soil Enzyme Activities in North China |
| title_full | Effects of Manure and Chemical Fertilizer on Bacterial Community Structure and Soil Enzyme Activities in North China |
| title_fullStr | Effects of Manure and Chemical Fertilizer on Bacterial Community Structure and Soil Enzyme Activities in North China |
| title_full_unstemmed | Effects of Manure and Chemical Fertilizer on Bacterial Community Structure and Soil Enzyme Activities in North China |
| title_short | Effects of Manure and Chemical Fertilizer on Bacterial Community Structure and Soil Enzyme Activities in North China |
| title_sort | effects of manure and chemical fertilizer on bacterial community structure and soil enzyme activities in north china |
| topic | bacterial community long-term fertilization soil enzyme activities high-throughput sequencing |
| url | https://www.mdpi.com/2073-4395/11/5/1017 |
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