Evaluation of a collagen-chitosan hydrogel for potential use as a pro-angiogenic site for islet transplantation.
Islet transplantation to treat type 1 diabetes (T1D) has shown varied long-term success, due in part to insufficient blood supply to maintain the islets. In the current study, collagen and collagen:chitosan (10:1) hydrogels, +/- circulating angiogenic cells (CACs), were compared for their ability to...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3799615?pdf=render |
| _version_ | 1819315496863924224 |
|---|---|
| author | Joanne E McBane Branka Vulesevic Donna T Padavan Kimberly A McEwan Gregory S Korbutt Erik J Suuronen |
| author_facet | Joanne E McBane Branka Vulesevic Donna T Padavan Kimberly A McEwan Gregory S Korbutt Erik J Suuronen |
| author_sort | Joanne E McBane |
| collection | DOAJ |
| description | Islet transplantation to treat type 1 diabetes (T1D) has shown varied long-term success, due in part to insufficient blood supply to maintain the islets. In the current study, collagen and collagen:chitosan (10:1) hydrogels, +/- circulating angiogenic cells (CACs), were compared for their ability to produce a pro-angiogenic environment in a streptozotocin-induced mouse model of T1D. Initial characterization showed that collagen-chitosan gels were mechanically stronger than the collagen gels (0.7 kPa vs. 0.4 kPa elastic modulus, respectively), had more cross-links (9.2 vs. 7.4/µm(2)), and were degraded more slowly by collagenase. After gelation with CACs, live/dead staining showed greater CAC viability in the collagen-chitosan gels after 18 h compared to collagen (79% vs. 69%). In vivo, collagen-chitosan gels, subcutaneously implanted for up to 6 weeks in a T1D mouse, showed increased levels of pro-angiogenic cytokines over time. By 6 weeks, anti-islet cytokine levels were decreased in all matrix formulations ± CACs. The 6-week implants demonstrated increased expression of VCAM-1 in collagen-chitosan implants. Despite this, infiltrating vWF(+) and CXCR4(+) angiogenic cell numbers were not different between the implant types, which may be due to a delayed and reduced cytokine response in a T1D versus non-diabetic setting. The mechanical, degradation and cytokine data all suggest that the collagen-chitosan gel may be a suitable candidate for use as a pro-angiogenic ectopic islet transplant site. |
| first_indexed | 2024-12-24T10:01:02Z |
| format | Article |
| id | doaj.art-00dd36c481aa4eb584fb84d4779cc63b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-24T10:01:02Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-00dd36c481aa4eb584fb84d4779cc63b2022-12-21T17:01:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7753810.1371/journal.pone.0077538Evaluation of a collagen-chitosan hydrogel for potential use as a pro-angiogenic site for islet transplantation.Joanne E McBaneBranka VulesevicDonna T PadavanKimberly A McEwanGregory S KorbuttErik J SuuronenIslet transplantation to treat type 1 diabetes (T1D) has shown varied long-term success, due in part to insufficient blood supply to maintain the islets. In the current study, collagen and collagen:chitosan (10:1) hydrogels, +/- circulating angiogenic cells (CACs), were compared for their ability to produce a pro-angiogenic environment in a streptozotocin-induced mouse model of T1D. Initial characterization showed that collagen-chitosan gels were mechanically stronger than the collagen gels (0.7 kPa vs. 0.4 kPa elastic modulus, respectively), had more cross-links (9.2 vs. 7.4/µm(2)), and were degraded more slowly by collagenase. After gelation with CACs, live/dead staining showed greater CAC viability in the collagen-chitosan gels after 18 h compared to collagen (79% vs. 69%). In vivo, collagen-chitosan gels, subcutaneously implanted for up to 6 weeks in a T1D mouse, showed increased levels of pro-angiogenic cytokines over time. By 6 weeks, anti-islet cytokine levels were decreased in all matrix formulations ± CACs. The 6-week implants demonstrated increased expression of VCAM-1 in collagen-chitosan implants. Despite this, infiltrating vWF(+) and CXCR4(+) angiogenic cell numbers were not different between the implant types, which may be due to a delayed and reduced cytokine response in a T1D versus non-diabetic setting. The mechanical, degradation and cytokine data all suggest that the collagen-chitosan gel may be a suitable candidate for use as a pro-angiogenic ectopic islet transplant site.http://europepmc.org/articles/PMC3799615?pdf=render |
| spellingShingle | Joanne E McBane Branka Vulesevic Donna T Padavan Kimberly A McEwan Gregory S Korbutt Erik J Suuronen Evaluation of a collagen-chitosan hydrogel for potential use as a pro-angiogenic site for islet transplantation. PLoS ONE |
| title | Evaluation of a collagen-chitosan hydrogel for potential use as a pro-angiogenic site for islet transplantation. |
| title_full | Evaluation of a collagen-chitosan hydrogel for potential use as a pro-angiogenic site for islet transplantation. |
| title_fullStr | Evaluation of a collagen-chitosan hydrogel for potential use as a pro-angiogenic site for islet transplantation. |
| title_full_unstemmed | Evaluation of a collagen-chitosan hydrogel for potential use as a pro-angiogenic site for islet transplantation. |
| title_short | Evaluation of a collagen-chitosan hydrogel for potential use as a pro-angiogenic site for islet transplantation. |
| title_sort | evaluation of a collagen chitosan hydrogel for potential use as a pro angiogenic site for islet transplantation |
| url | http://europepmc.org/articles/PMC3799615?pdf=render |
| work_keys_str_mv | AT joanneemcbane evaluationofacollagenchitosanhydrogelforpotentialuseasaproangiogenicsiteforislettransplantation AT brankavulesevic evaluationofacollagenchitosanhydrogelforpotentialuseasaproangiogenicsiteforislettransplantation AT donnatpadavan evaluationofacollagenchitosanhydrogelforpotentialuseasaproangiogenicsiteforislettransplantation AT kimberlyamcewan evaluationofacollagenchitosanhydrogelforpotentialuseasaproangiogenicsiteforislettransplantation AT gregoryskorbutt evaluationofacollagenchitosanhydrogelforpotentialuseasaproangiogenicsiteforislettransplantation AT erikjsuuronen evaluationofacollagenchitosanhydrogelforpotentialuseasaproangiogenicsiteforislettransplantation |