Synergistic Antibacterial and Anti-inflammatory Activities of <i>Ocimum tenuiflorum</i> Ethanolic Extract against Major Bacterial Mastitis Pathogens
Mastitis is the most prevalent global illness affecting dairy cows. This bacterial infection damages and inflames the udder tissues. Several plant extracts have demonstrated synergistic antibacterial activities with standard drugs in mastitis treatment. Scant information exists on <i>Ocimum te...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/4/510 |
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| author | Janejira Srichok Natthika Yingbun Teerada Kowawisetsut Sudsaijai Kornmatitsuk Uthaiwan Suttisansanee Piya Temviriyanukul Boonrat Chantong |
| author_facet | Janejira Srichok Natthika Yingbun Teerada Kowawisetsut Sudsaijai Kornmatitsuk Uthaiwan Suttisansanee Piya Temviriyanukul Boonrat Chantong |
| author_sort | Janejira Srichok |
| collection | DOAJ |
| description | Mastitis is the most prevalent global illness affecting dairy cows. This bacterial infection damages and inflames the udder tissues. Several plant extracts have demonstrated synergistic antibacterial activities with standard drugs in mastitis treatment. Scant information exists on <i>Ocimum tenuiflorum</i> L. This study evaluated the antibacterial activity of <i>O. tenuiflorum</i> extract and its interaction with antibacterial drugs against common mastitis pathogens including <i>Staphylococcus aureus</i>, coagulase-negative Staphylococci (CNS), <i>Streptococcus agalactiae</i>, and <i>Escherichia coli</i>. Anti-inflammatory activities in LPS-stimulated RAW264.7 macrophage cells were also studied. The <i>O. tenuiflorum</i> extract exhibited antibacterial activities against <i>S. aureus</i>, CNS, and <i>S. agalactiae</i> with minimum inhibitory concentration (MIC) ranging from 3.9 to 31.2 µg/mL and minimum bactericidal concentration (MBC) ranging from 15.6 to 500 µg/mL. Combinations of <i>O. tenuiflorum</i> with penicillin or amoxicillin-clavulanic acid showed synergistic effects against all tested strains but an additive effect with cefazolin and gentamicin. Pretreatment of the extract significantly decreased the expression of inflammatory molecules (IL-6, TNF-α, IL-1β, iNOS, COX-2, and PGE2) generated by LPS in macrophages. Results suggested <i>O. tenuiflorum</i> effectiveness against various Gram-positive mastitis bacteria, with the potential to reduce antibacterial doses and combat inflammation. |
| first_indexed | 2024-03-09T11:14:32Z |
| format | Article |
| id | doaj.art-00e011b097f64d279639c726ece65ba8 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T11:14:32Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-00e011b097f64d279639c726ece65ba82023-12-01T00:33:07ZengMDPI AGAntibiotics2079-63822022-04-0111451010.3390/antibiotics11040510Synergistic Antibacterial and Anti-inflammatory Activities of <i>Ocimum tenuiflorum</i> Ethanolic Extract against Major Bacterial Mastitis PathogensJanejira Srichok0Natthika Yingbun1Teerada Kowawisetsut2Sudsaijai Kornmatitsuk3Uthaiwan Suttisansanee4Piya Temviriyanukul5Boonrat Chantong6Faculty of Veterinary Science, Mahidol University, Salaya, Phutthamonthon, Nakhon Pathom 73170, ThailandFaculty of Veterinary Science, Mahidol University, Salaya, Phutthamonthon, Nakhon Pathom 73170, ThailandFaculty of Veterinary Science, Mahidol University, Salaya, Phutthamonthon, Nakhon Pathom 73170, ThailandDepartment of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, ThailandDepartment of Pre-Clinical and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, ThailandMastitis is the most prevalent global illness affecting dairy cows. This bacterial infection damages and inflames the udder tissues. Several plant extracts have demonstrated synergistic antibacterial activities with standard drugs in mastitis treatment. Scant information exists on <i>Ocimum tenuiflorum</i> L. This study evaluated the antibacterial activity of <i>O. tenuiflorum</i> extract and its interaction with antibacterial drugs against common mastitis pathogens including <i>Staphylococcus aureus</i>, coagulase-negative Staphylococci (CNS), <i>Streptococcus agalactiae</i>, and <i>Escherichia coli</i>. Anti-inflammatory activities in LPS-stimulated RAW264.7 macrophage cells were also studied. The <i>O. tenuiflorum</i> extract exhibited antibacterial activities against <i>S. aureus</i>, CNS, and <i>S. agalactiae</i> with minimum inhibitory concentration (MIC) ranging from 3.9 to 31.2 µg/mL and minimum bactericidal concentration (MBC) ranging from 15.6 to 500 µg/mL. Combinations of <i>O. tenuiflorum</i> with penicillin or amoxicillin-clavulanic acid showed synergistic effects against all tested strains but an additive effect with cefazolin and gentamicin. Pretreatment of the extract significantly decreased the expression of inflammatory molecules (IL-6, TNF-α, IL-1β, iNOS, COX-2, and PGE2) generated by LPS in macrophages. Results suggested <i>O. tenuiflorum</i> effectiveness against various Gram-positive mastitis bacteria, with the potential to reduce antibacterial doses and combat inflammation.https://www.mdpi.com/2079-6382/11/4/510antibacterial activityanti-inflammationmastitis<i>Ocimum tenuiflorum</i> L.synergistic |
| spellingShingle | Janejira Srichok Natthika Yingbun Teerada Kowawisetsut Sudsaijai Kornmatitsuk Uthaiwan Suttisansanee Piya Temviriyanukul Boonrat Chantong Synergistic Antibacterial and Anti-inflammatory Activities of <i>Ocimum tenuiflorum</i> Ethanolic Extract against Major Bacterial Mastitis Pathogens Antibiotics antibacterial activity anti-inflammation mastitis <i>Ocimum tenuiflorum</i> L. synergistic |
| title | Synergistic Antibacterial and Anti-inflammatory Activities of <i>Ocimum tenuiflorum</i> Ethanolic Extract against Major Bacterial Mastitis Pathogens |
| title_full | Synergistic Antibacterial and Anti-inflammatory Activities of <i>Ocimum tenuiflorum</i> Ethanolic Extract against Major Bacterial Mastitis Pathogens |
| title_fullStr | Synergistic Antibacterial and Anti-inflammatory Activities of <i>Ocimum tenuiflorum</i> Ethanolic Extract against Major Bacterial Mastitis Pathogens |
| title_full_unstemmed | Synergistic Antibacterial and Anti-inflammatory Activities of <i>Ocimum tenuiflorum</i> Ethanolic Extract against Major Bacterial Mastitis Pathogens |
| title_short | Synergistic Antibacterial and Anti-inflammatory Activities of <i>Ocimum tenuiflorum</i> Ethanolic Extract against Major Bacterial Mastitis Pathogens |
| title_sort | synergistic antibacterial and anti inflammatory activities of i ocimum tenuiflorum i ethanolic extract against major bacterial mastitis pathogens |
| topic | antibacterial activity anti-inflammation mastitis <i>Ocimum tenuiflorum</i> L. synergistic |
| url | https://www.mdpi.com/2079-6382/11/4/510 |
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