A conserved aspartic acid is important for agonist (VUAA1) and odorant/tuning receptor-dependent activation of the insect odorant co-receptor (Orco).
Insect odorant receptors function as heteromeric odorant-gated cation channels comprising a conventional odorant-sensitive tuning receptor, and a conserved co-receptor (Orco). An Orco agonist, VUAA1, is able to activate both heteromeric and homomeric Orco-containing channels. Very little is known ab...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3720905?pdf=render |
_version_ | 1811294105612845056 |
---|---|
author | Brijesh N Kumar Robert W Taylor Gregory M Pask Laurence J Zwiebel Richard D Newcomb David L Christie |
author_facet | Brijesh N Kumar Robert W Taylor Gregory M Pask Laurence J Zwiebel Richard D Newcomb David L Christie |
author_sort | Brijesh N Kumar |
collection | DOAJ |
description | Insect odorant receptors function as heteromeric odorant-gated cation channels comprising a conventional odorant-sensitive tuning receptor, and a conserved co-receptor (Orco). An Orco agonist, VUAA1, is able to activate both heteromeric and homomeric Orco-containing channels. Very little is known about specific residues in Orco that contribute to cation permeability and gating. We investigated the importance of two conserved Asp residues, one in each of transmembrane domains 5 and 7, for channel function by mutagenesis. Drosophila melanogaster Orco and its substitution mutants were expressed in HEK cells and VUAA1-stimulated channel activity was determined by Ca(2+) influx and whole-cell patch clamp electrophysiology. Substitution of D466 in transmembrane 7 with amino acids other than glutamic acid resulted in a substantial reduction in channel activity. The D466E Orco substitution mutant was ~2 times more sensitive to VUAA1. The permeability of the D466E Orco mutant to cations was unchanged relative to wild-type Orco. When D466E Orco is co-expressed with a conventional tuning odorant receptor, the heteromeric complex also shows increased sensitivity to an odorant. Thus, the effect of the D466E mutation is not specific to VUAA1 agonism or dependent on homomeric Orco assembly. We suggest the gain-of-activation characteristic of the D466E mutant identifies an amino acid that is likely to be important for activation of both heteromeric and homomeric insect odorant receptor channels. |
first_indexed | 2024-04-13T05:11:34Z |
format | Article |
id | doaj.art-00e05733b5554d989d4d295b11bced55 |
institution | Directory Open Access Journal |
issn | 1932-6203 |
language | English |
last_indexed | 2024-04-13T05:11:34Z |
publishDate | 2013-01-01 |
publisher | Public Library of Science (PLoS) |
record_format | Article |
series | PLoS ONE |
spelling | doaj.art-00e05733b5554d989d4d295b11bced552022-12-22T03:01:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0187e7021810.1371/journal.pone.0070218A conserved aspartic acid is important for agonist (VUAA1) and odorant/tuning receptor-dependent activation of the insect odorant co-receptor (Orco).Brijesh N KumarRobert W TaylorGregory M PaskLaurence J ZwiebelRichard D NewcombDavid L ChristieInsect odorant receptors function as heteromeric odorant-gated cation channels comprising a conventional odorant-sensitive tuning receptor, and a conserved co-receptor (Orco). An Orco agonist, VUAA1, is able to activate both heteromeric and homomeric Orco-containing channels. Very little is known about specific residues in Orco that contribute to cation permeability and gating. We investigated the importance of two conserved Asp residues, one in each of transmembrane domains 5 and 7, for channel function by mutagenesis. Drosophila melanogaster Orco and its substitution mutants were expressed in HEK cells and VUAA1-stimulated channel activity was determined by Ca(2+) influx and whole-cell patch clamp electrophysiology. Substitution of D466 in transmembrane 7 with amino acids other than glutamic acid resulted in a substantial reduction in channel activity. The D466E Orco substitution mutant was ~2 times more sensitive to VUAA1. The permeability of the D466E Orco mutant to cations was unchanged relative to wild-type Orco. When D466E Orco is co-expressed with a conventional tuning odorant receptor, the heteromeric complex also shows increased sensitivity to an odorant. Thus, the effect of the D466E mutation is not specific to VUAA1 agonism or dependent on homomeric Orco assembly. We suggest the gain-of-activation characteristic of the D466E mutant identifies an amino acid that is likely to be important for activation of both heteromeric and homomeric insect odorant receptor channels.http://europepmc.org/articles/PMC3720905?pdf=render |
spellingShingle | Brijesh N Kumar Robert W Taylor Gregory M Pask Laurence J Zwiebel Richard D Newcomb David L Christie A conserved aspartic acid is important for agonist (VUAA1) and odorant/tuning receptor-dependent activation of the insect odorant co-receptor (Orco). PLoS ONE |
title | A conserved aspartic acid is important for agonist (VUAA1) and odorant/tuning receptor-dependent activation of the insect odorant co-receptor (Orco). |
title_full | A conserved aspartic acid is important for agonist (VUAA1) and odorant/tuning receptor-dependent activation of the insect odorant co-receptor (Orco). |
title_fullStr | A conserved aspartic acid is important for agonist (VUAA1) and odorant/tuning receptor-dependent activation of the insect odorant co-receptor (Orco). |
title_full_unstemmed | A conserved aspartic acid is important for agonist (VUAA1) and odorant/tuning receptor-dependent activation of the insect odorant co-receptor (Orco). |
title_short | A conserved aspartic acid is important for agonist (VUAA1) and odorant/tuning receptor-dependent activation of the insect odorant co-receptor (Orco). |
title_sort | conserved aspartic acid is important for agonist vuaa1 and odorant tuning receptor dependent activation of the insect odorant co receptor orco |
url | http://europepmc.org/articles/PMC3720905?pdf=render |
work_keys_str_mv | AT brijeshnkumar aconservedasparticacidisimportantforagonistvuaa1andodoranttuningreceptordependentactivationoftheinsectodorantcoreceptororco AT robertwtaylor aconservedasparticacidisimportantforagonistvuaa1andodoranttuningreceptordependentactivationoftheinsectodorantcoreceptororco AT gregorympask aconservedasparticacidisimportantforagonistvuaa1andodoranttuningreceptordependentactivationoftheinsectodorantcoreceptororco AT laurencejzwiebel aconservedasparticacidisimportantforagonistvuaa1andodoranttuningreceptordependentactivationoftheinsectodorantcoreceptororco AT richarddnewcomb aconservedasparticacidisimportantforagonistvuaa1andodoranttuningreceptordependentactivationoftheinsectodorantcoreceptororco AT davidlchristie aconservedasparticacidisimportantforagonistvuaa1andodoranttuningreceptordependentactivationoftheinsectodorantcoreceptororco AT brijeshnkumar conservedasparticacidisimportantforagonistvuaa1andodoranttuningreceptordependentactivationoftheinsectodorantcoreceptororco AT robertwtaylor conservedasparticacidisimportantforagonistvuaa1andodoranttuningreceptordependentactivationoftheinsectodorantcoreceptororco AT gregorympask conservedasparticacidisimportantforagonistvuaa1andodoranttuningreceptordependentactivationoftheinsectodorantcoreceptororco AT laurencejzwiebel conservedasparticacidisimportantforagonistvuaa1andodoranttuningreceptordependentactivationoftheinsectodorantcoreceptororco AT richarddnewcomb conservedasparticacidisimportantforagonistvuaa1andodoranttuningreceptordependentactivationoftheinsectodorantcoreceptororco AT davidlchristie conservedasparticacidisimportantforagonistvuaa1andodoranttuningreceptordependentactivationoftheinsectodorantcoreceptororco |