Hemispherical Microelectrode Array for Ex Vivo Retinal Neural Recording
To investigate the neuronal visual encoding process in the retina, researchers have performed in vitro and ex vivo electrophysiological experiments using animal retinal tissues. The microelectrode array (MEA) has become a key component in retinal experiments because it enables simultaneous neural re...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-05-01
|
| Series: | Micromachines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-666X/11/5/538 |
| _version_ | 1797567058022498304 |
|---|---|
| author | Yoonhee Ha Hyun-Ji Yoo Soowon Shin Sang Beom Jun |
| author_facet | Yoonhee Ha Hyun-Ji Yoo Soowon Shin Sang Beom Jun |
| author_sort | Yoonhee Ha |
| collection | DOAJ |
| description | To investigate the neuronal visual encoding process in the retina, researchers have performed in vitro and ex vivo electrophysiological experiments using animal retinal tissues. The microelectrode array (MEA) has become a key component in retinal experiments because it enables simultaneous neural recording from a population of retinal neurons. However, in most retinal experiments, it is inevitable that the retinal tissue is flattened on the planar MEA, becoming deformed from the original hemispherical shape. During the tissue deforming process, the retina is subjected to mechanical stress, which can induce abnormal physiological conditions. To overcome this problem, in this study, we propose a hemispherical MEA with a curvature that allows retinal tissues to adhere closely to electrodes without tissue deformation. The electrode array is fabricated by stretching a thin, flexible polydimethylsiloxane (PDMS) electrode layer onto a hemispherical substrate. To form micro patterns of electrodes, laser processing is employed instead of conventional thin-film microfabrication processes. The feasibility for neural recording from retinal tissues using this array is shown by conducting ex vivo retinal experiments. We anticipate that the proposed techniques for hemispherical MEAs can be utilized not only for ex vivo retinal studies but also for various flexible electronics. |
| first_indexed | 2024-03-10T19:35:16Z |
| format | Article |
| id | doaj.art-00e223065f92489aba24e4a8625ebf6c |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T19:35:16Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-00e223065f92489aba24e4a8625ebf6c2023-11-20T01:41:23ZengMDPI AGMicromachines2072-666X2020-05-0111553810.3390/mi11050538Hemispherical Microelectrode Array for Ex Vivo Retinal Neural RecordingYoonhee Ha0Hyun-Ji Yoo1Soowon Shin2Sang Beom Jun3Department of Electronic and Electrical Engineering, Ewha Womans University, Seoul 03760, KoreaDepartment of Electronic and Electrical Engineering, Ewha Womans University, Seoul 03760, KoreaDepartment of Bioengineering, TODOC Co., Ltd., Seoul 08394, KoreaDepartment of Electronic and Electrical Engineering, Ewha Womans University, Seoul 03760, KoreaTo investigate the neuronal visual encoding process in the retina, researchers have performed in vitro and ex vivo electrophysiological experiments using animal retinal tissues. The microelectrode array (MEA) has become a key component in retinal experiments because it enables simultaneous neural recording from a population of retinal neurons. However, in most retinal experiments, it is inevitable that the retinal tissue is flattened on the planar MEA, becoming deformed from the original hemispherical shape. During the tissue deforming process, the retina is subjected to mechanical stress, which can induce abnormal physiological conditions. To overcome this problem, in this study, we propose a hemispherical MEA with a curvature that allows retinal tissues to adhere closely to electrodes without tissue deformation. The electrode array is fabricated by stretching a thin, flexible polydimethylsiloxane (PDMS) electrode layer onto a hemispherical substrate. To form micro patterns of electrodes, laser processing is employed instead of conventional thin-film microfabrication processes. The feasibility for neural recording from retinal tissues using this array is shown by conducting ex vivo retinal experiments. We anticipate that the proposed techniques for hemispherical MEAs can be utilized not only for ex vivo retinal studies but also for various flexible electronics.https://www.mdpi.com/2072-666X/11/5/538microelectrode array (MEA)hemisphericalretinaneural recording |
| spellingShingle | Yoonhee Ha Hyun-Ji Yoo Soowon Shin Sang Beom Jun Hemispherical Microelectrode Array for Ex Vivo Retinal Neural Recording Micromachines microelectrode array (MEA) hemispherical retina neural recording |
| title | Hemispherical Microelectrode Array for Ex Vivo Retinal Neural Recording |
| title_full | Hemispherical Microelectrode Array for Ex Vivo Retinal Neural Recording |
| title_fullStr | Hemispherical Microelectrode Array for Ex Vivo Retinal Neural Recording |
| title_full_unstemmed | Hemispherical Microelectrode Array for Ex Vivo Retinal Neural Recording |
| title_short | Hemispherical Microelectrode Array for Ex Vivo Retinal Neural Recording |
| title_sort | hemispherical microelectrode array for ex vivo retinal neural recording |
| topic | microelectrode array (MEA) hemispherical retina neural recording |
| url | https://www.mdpi.com/2072-666X/11/5/538 |
| work_keys_str_mv | AT yoonheeha hemisphericalmicroelectrodearrayforexvivoretinalneuralrecording AT hyunjiyoo hemisphericalmicroelectrodearrayforexvivoretinalneuralrecording AT soowonshin hemisphericalmicroelectrodearrayforexvivoretinalneuralrecording AT sangbeomjun hemisphericalmicroelectrodearrayforexvivoretinalneuralrecording |