Bioactive Potential of Extracts of <i>Labrenzia aggregata</i> Strain USBA 371, a Halophilic Bacterium Isolated from a Terrestrial Source
Previous studies revealed the potential of <i>Labrenzia aggregata</i> USBA 371 to produce cytotoxic metabolites. This study explores its metabolic diversity and compounds involved in its cytotoxic activity. Extracts from the extracellular fraction of strain USBA 371 showed high levels of...
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MDPI AG
2020-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/11/2546 |
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| author | Carolina Díaz-Cárdenas Laura Yinneth Rojas Susana Fiorentino Monica P. Cala Jorge I Díaz Freddy A. Ramos Jean Armengaud Silvia Restrepo Sandra Baena |
| author_facet | Carolina Díaz-Cárdenas Laura Yinneth Rojas Susana Fiorentino Monica P. Cala Jorge I Díaz Freddy A. Ramos Jean Armengaud Silvia Restrepo Sandra Baena |
| author_sort | Carolina Díaz-Cárdenas |
| collection | DOAJ |
| description | Previous studies revealed the potential of <i>Labrenzia aggregata</i> USBA 371 to produce cytotoxic metabolites. This study explores its metabolic diversity and compounds involved in its cytotoxic activity. Extracts from the extracellular fraction of strain USBA 371 showed high levels of cytotoxic activity associated with the production of diketopiperazines (DKPs). We purified two compounds and a mixture of two other compounds from this fraction. Their structures were characterized by 1D and 2D nuclear magnetic resonance (NMR). The purified compounds were evaluated for additional cytotoxic activities. Compound <b>1</b> (cyclo (<span style="font-variant: small-caps;">l</span>-Pro-<span style="font-variant: small-caps;">l</span>-Tyr)) showed cytotoxicity to the following cancer cell lines: breast cancer 4T1 (IC<sub>50</sub> 57.09 ± 2.11 µM), 4T1H17 (IC<sub>50</sub> 40.38 ± 1.94), MCF-7 (IC<sub>50</sub> 87.74 ± 2.32 µM), murine melanoma B16 (IC<sub>50</sub> 80.87 ± 3.67), human uterus sarcoma MES-SA/Dx5 P-pg (−) (IC<sub>50</sub> 291.32 ± 5.64) and MES-SA/Dx5 P-pg (+) (IC<sub>50</sub> 225.28 ± 1.23), and murine colon MCA 38 (IC<sub>50</sub> 29.85 ± 1.55). In order to elucidate the biosynthetic route of the production of DKPs and other secondary metabolites, we sequenced the genome of <i>L. aggregata</i> USBA 371. We found no evidence for biosynthetic pathways associated with cyclodipeptide synthases (CDPSs) or non-ribosomal peptides (NRPS), but based on proteogenomic analysis we suggest that they are produced by proteolytic enzymes. This is the first report in which the cytotoxic effect of cyclo (<span style="font-variant: small-caps;">l</span>-Pro-<span style="font-variant: small-caps;">l</span>-Tyr) produced by an organism of the genus <i>Labrenzia</i> has been evaluated against several cancer cell lines. |
| first_indexed | 2024-03-10T19:29:16Z |
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| id | doaj.art-00e309f5ff2546cea02221d0bef143a8 |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T19:29:16Z |
| publishDate | 2020-05-01 |
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| spelling | doaj.art-00e309f5ff2546cea02221d0bef143a82023-11-20T02:14:08ZengMDPI AGMolecules1420-30492020-05-012511254610.3390/molecules25112546Bioactive Potential of Extracts of <i>Labrenzia aggregata</i> Strain USBA 371, a Halophilic Bacterium Isolated from a Terrestrial SourceCarolina Díaz-Cárdenas0Laura Yinneth Rojas1Susana Fiorentino2Monica P. Cala3Jorge I Díaz4Freddy A. Ramos5Jean Armengaud6Silvia Restrepo7Sandra Baena8Unidad de Saneamiento y Biotecnología Ambiental, Departamento de Biología, Pontificia Universidad Javeriana, P.O. Box 56710 Bogotá DC, ColombiaGrupo de Inmunobiología y Biología Celular, Pontificia Universidad Javeriana, P.O. Box 56710 Bogotá DC, ColombiaGrupo de Inmunobiología y Biología Celular, Pontificia Universidad Javeriana, P.O. Box 56710 Bogotá DC, ColombiaMetabolomics Core Facility—MetCore, Vicepresidency for Research and Creation, Universidad de los Andes, Cra 1 No. 18A-12, 111711 Bogotá DC, ColombiaVicepresidency of Research and Creation, Universidad de los Andes, Cra 1 No. 18A-12, 111711 Bogotá DC, ColombiaDepartamento de Química, Facultad de Ciencias, Universidad Nacional de Colombia-Sede Bogotá, Carrera 30 # 45-03, 110111 Bogotá DC, ColombiaDépartement Médicaments et Technologies pour la Santé (DMTS), CEA, INRAE, Université Paris Saclay, SPI, 30200 Bagnols-sur-Cèze, FranceChemical Engineering Department, Universidad de los Andes, Cra 1 No. 18A-12, 111711 Bogotá DC, ColombiaUnidad de Saneamiento y Biotecnología Ambiental, Departamento de Biología, Pontificia Universidad Javeriana, P.O. Box 56710 Bogotá DC, ColombiaPrevious studies revealed the potential of <i>Labrenzia aggregata</i> USBA 371 to produce cytotoxic metabolites. This study explores its metabolic diversity and compounds involved in its cytotoxic activity. Extracts from the extracellular fraction of strain USBA 371 showed high levels of cytotoxic activity associated with the production of diketopiperazines (DKPs). We purified two compounds and a mixture of two other compounds from this fraction. Their structures were characterized by 1D and 2D nuclear magnetic resonance (NMR). The purified compounds were evaluated for additional cytotoxic activities. Compound <b>1</b> (cyclo (<span style="font-variant: small-caps;">l</span>-Pro-<span style="font-variant: small-caps;">l</span>-Tyr)) showed cytotoxicity to the following cancer cell lines: breast cancer 4T1 (IC<sub>50</sub> 57.09 ± 2.11 µM), 4T1H17 (IC<sub>50</sub> 40.38 ± 1.94), MCF-7 (IC<sub>50</sub> 87.74 ± 2.32 µM), murine melanoma B16 (IC<sub>50</sub> 80.87 ± 3.67), human uterus sarcoma MES-SA/Dx5 P-pg (−) (IC<sub>50</sub> 291.32 ± 5.64) and MES-SA/Dx5 P-pg (+) (IC<sub>50</sub> 225.28 ± 1.23), and murine colon MCA 38 (IC<sub>50</sub> 29.85 ± 1.55). In order to elucidate the biosynthetic route of the production of DKPs and other secondary metabolites, we sequenced the genome of <i>L. aggregata</i> USBA 371. We found no evidence for biosynthetic pathways associated with cyclodipeptide synthases (CDPSs) or non-ribosomal peptides (NRPS), but based on proteogenomic analysis we suggest that they are produced by proteolytic enzymes. This is the first report in which the cytotoxic effect of cyclo (<span style="font-variant: small-caps;">l</span>-Pro-<span style="font-variant: small-caps;">l</span>-Tyr) produced by an organism of the genus <i>Labrenzia</i> has been evaluated against several cancer cell lines.https://www.mdpi.com/1420-3049/25/11/2546<i>Labrenzia aggregata</i> USBA 371cytotoxic activitiesbioactive natural productssaline natural productssecondary metabolismgenome annotation |
| spellingShingle | Carolina Díaz-Cárdenas Laura Yinneth Rojas Susana Fiorentino Monica P. Cala Jorge I Díaz Freddy A. Ramos Jean Armengaud Silvia Restrepo Sandra Baena Bioactive Potential of Extracts of <i>Labrenzia aggregata</i> Strain USBA 371, a Halophilic Bacterium Isolated from a Terrestrial Source Molecules <i>Labrenzia aggregata</i> USBA 371 cytotoxic activities bioactive natural products saline natural products secondary metabolism genome annotation |
| title | Bioactive Potential of Extracts of <i>Labrenzia aggregata</i> Strain USBA 371, a Halophilic Bacterium Isolated from a Terrestrial Source |
| title_full | Bioactive Potential of Extracts of <i>Labrenzia aggregata</i> Strain USBA 371, a Halophilic Bacterium Isolated from a Terrestrial Source |
| title_fullStr | Bioactive Potential of Extracts of <i>Labrenzia aggregata</i> Strain USBA 371, a Halophilic Bacterium Isolated from a Terrestrial Source |
| title_full_unstemmed | Bioactive Potential of Extracts of <i>Labrenzia aggregata</i> Strain USBA 371, a Halophilic Bacterium Isolated from a Terrestrial Source |
| title_short | Bioactive Potential of Extracts of <i>Labrenzia aggregata</i> Strain USBA 371, a Halophilic Bacterium Isolated from a Terrestrial Source |
| title_sort | bioactive potential of extracts of i labrenzia aggregata i strain usba 371 a halophilic bacterium isolated from a terrestrial source |
| topic | <i>Labrenzia aggregata</i> USBA 371 cytotoxic activities bioactive natural products saline natural products secondary metabolism genome annotation |
| url | https://www.mdpi.com/1420-3049/25/11/2546 |
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