Antibacterial effects of chitosan-based hydrogels containing Trachyspermum ammi essential oil on pathogens isolated from dogs with otitis externa
Abstract Background Growing antibiotic resistance has made treating otitis externa (OE) increasingly challenging. On the other hand, local antimicrobial treatments, especially those that combine essential oils (EOs) with nanoparticles, tend to be preferred over systemic ones. It was investigated whe...
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-024-03971-7 |
| _version_ | 1797219706423214080 |
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| author | Niloofar Jelokhani Niaraki Shahram Jamshidi Bahar Nayeri Fasaei Seyed Mehdi Joghataei |
| author_facet | Niloofar Jelokhani Niaraki Shahram Jamshidi Bahar Nayeri Fasaei Seyed Mehdi Joghataei |
| author_sort | Niloofar Jelokhani Niaraki |
| collection | DOAJ |
| description | Abstract Background Growing antibiotic resistance has made treating otitis externa (OE) increasingly challenging. On the other hand, local antimicrobial treatments, especially those that combine essential oils (EOs) with nanoparticles, tend to be preferred over systemic ones. It was investigated whether Ajwain (Trachyspermum ammi) EO, combined with chitosan nanoparticles modified by cholesterol, could inhibit the growth of bacterial pathogens isolated from OE cases in dogs. In total, 57 dogs with clinical signs of OE were examined and bacteriologically tested. Hydrogels of Chitosan were synthesized by self-assembly and investigated. EO was extracted (Clevenger machine), and its ingredients were checked (GC-MS analysis) and encapsulated in chitosan-cholesterol nanoparticles. Disc-diffusion and broth Micro-dilution (MIC and MBC) examined its antimicrobial and therapeutic properties. Results Staphylococcus pseudintermedius (49.3%) was the most common bacteria isolated from OE cases, followed by Pseudomonas aeruginosa (14.7%), Escherichia coli (13.3%), Streptococcus canis (9.3%), Corynebacterium auriscanis (6.7%), Klebsiella pneumoniae (2.7%), Proteus mirabilis (2.7%), and Bacillus cereus (1.3%). The investigation into the antimicrobial properties of Ajwain EO encapsulated in chitosan nanoparticles revealed that it exhibited a more pronounced antimicrobial effect against the pathogens responsible for OE. Conclusions Using chitosan nanoparticles encapsulated with EO presents an effective treatment approach for dogs with OE that conventional antimicrobial treatments have not cured. This approach not only enhances antibacterial effects but also reduces the required dosage of antimicrobials, potentially preventing the emergence of antimicrobial resistance. |
| first_indexed | 2024-04-24T12:37:54Z |
| format | Article |
| id | doaj.art-00e348dd9d724bb9b4365f66e9a9b387 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-24T12:37:54Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-00e348dd9d724bb9b4365f66e9a9b3872024-04-07T11:21:40ZengBMCBMC Veterinary Research1746-61482024-04-0120111110.1186/s12917-024-03971-7Antibacterial effects of chitosan-based hydrogels containing Trachyspermum ammi essential oil on pathogens isolated from dogs with otitis externaNiloofar Jelokhani Niaraki0Shahram Jamshidi1Bahar Nayeri Fasaei2Seyed Mehdi Joghataei3Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of TehranDepartment of Internal Medicine, Faculty of Veterinary Medicine, University of TehranDepartment of Microbiology and Immunology, Faculty of Veterinary Medicine, University of TehranDepartment of Microbiology and Immunology, Faculty of Veterinary Medicine, University of TehranAbstract Background Growing antibiotic resistance has made treating otitis externa (OE) increasingly challenging. On the other hand, local antimicrobial treatments, especially those that combine essential oils (EOs) with nanoparticles, tend to be preferred over systemic ones. It was investigated whether Ajwain (Trachyspermum ammi) EO, combined with chitosan nanoparticles modified by cholesterol, could inhibit the growth of bacterial pathogens isolated from OE cases in dogs. In total, 57 dogs with clinical signs of OE were examined and bacteriologically tested. Hydrogels of Chitosan were synthesized by self-assembly and investigated. EO was extracted (Clevenger machine), and its ingredients were checked (GC-MS analysis) and encapsulated in chitosan-cholesterol nanoparticles. Disc-diffusion and broth Micro-dilution (MIC and MBC) examined its antimicrobial and therapeutic properties. Results Staphylococcus pseudintermedius (49.3%) was the most common bacteria isolated from OE cases, followed by Pseudomonas aeruginosa (14.7%), Escherichia coli (13.3%), Streptococcus canis (9.3%), Corynebacterium auriscanis (6.7%), Klebsiella pneumoniae (2.7%), Proteus mirabilis (2.7%), and Bacillus cereus (1.3%). The investigation into the antimicrobial properties of Ajwain EO encapsulated in chitosan nanoparticles revealed that it exhibited a more pronounced antimicrobial effect against the pathogens responsible for OE. Conclusions Using chitosan nanoparticles encapsulated with EO presents an effective treatment approach for dogs with OE that conventional antimicrobial treatments have not cured. This approach not only enhances antibacterial effects but also reduces the required dosage of antimicrobials, potentially preventing the emergence of antimicrobial resistance.https://doi.org/10.1186/s12917-024-03971-7Essential oilTrachyspermum ammiOtitis externaNanoparticlesChitosanHydrogels |
| spellingShingle | Niloofar Jelokhani Niaraki Shahram Jamshidi Bahar Nayeri Fasaei Seyed Mehdi Joghataei Antibacterial effects of chitosan-based hydrogels containing Trachyspermum ammi essential oil on pathogens isolated from dogs with otitis externa BMC Veterinary Research Essential oil Trachyspermum ammi Otitis externa Nanoparticles Chitosan Hydrogels |
| title | Antibacterial effects of chitosan-based hydrogels containing Trachyspermum ammi essential oil on pathogens isolated from dogs with otitis externa |
| title_full | Antibacterial effects of chitosan-based hydrogels containing Trachyspermum ammi essential oil on pathogens isolated from dogs with otitis externa |
| title_fullStr | Antibacterial effects of chitosan-based hydrogels containing Trachyspermum ammi essential oil on pathogens isolated from dogs with otitis externa |
| title_full_unstemmed | Antibacterial effects of chitosan-based hydrogels containing Trachyspermum ammi essential oil on pathogens isolated from dogs with otitis externa |
| title_short | Antibacterial effects of chitosan-based hydrogels containing Trachyspermum ammi essential oil on pathogens isolated from dogs with otitis externa |
| title_sort | antibacterial effects of chitosan based hydrogels containing trachyspermum ammi essential oil on pathogens isolated from dogs with otitis externa |
| topic | Essential oil Trachyspermum ammi Otitis externa Nanoparticles Chitosan Hydrogels |
| url | https://doi.org/10.1186/s12917-024-03971-7 |
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