Mechanical compression attenuates normal human bronchial epithelial wound healing
<p>Abstract</p> <p>Background</p> <p>Airway narrowing associated with chronic asthma results in the transmission of injurious compressive forces to the bronchial epithelium and promotes the release of pro-inflammatory mediators and the denudation of the bronchial epithe...
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| Format: | Article |
| Language: | English |
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BMC
2009-02-01
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| Series: | Respiratory Research |
| Online Access: | http://www.biomedcentral.com/1465-9921/10/9 |
| _version_ | 1828259463641956352 |
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| author | Malavia Nikita Arold Stephen P George Steven C |
| author_facet | Malavia Nikita Arold Stephen P George Steven C |
| author_sort | Malavia Nikita |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Airway narrowing associated with chronic asthma results in the transmission of injurious compressive forces to the bronchial epithelium and promotes the release of pro-inflammatory mediators and the denudation of the bronchial epithelium. While the individual effects of compression or denudation are well characterized, there is no data to elucidate how these cells respond to the application of mechanical compression in the presence of a compromised epithelial layer.</p> <p>Methods</p> <p>Accordingly, differentiated normal human bronchial epithelial cells were exposed to one of four conditions: 1) unperturbed control cells, 2) single scrape wound only, 3) static compression (6 hours of 30 cmH<sub>2</sub>O), and 4) 6 hours of static compression after a scrape wound. Following treatment, wound closure rate was recorded, media was assayed for mediator content and the cytoskeletal network was fluorescently labeled.</p> <p>Results</p> <p>We found that mechanical compression and scrape injury increase TGF-β2 and endothelin-1 secretion, while EGF content in the media is attenuated with both injury modes. The application of compression after a pre-existing scrape wound augmented these observations, and also decreased PGE<sub>2 </sub>media content. Compression stimulated depolymerization of the actin cytoskeleton and significantly attenuated wound healing. Closure rate was partially restored with the addition of exogenous PGE<sub>2</sub>, but not EGF.</p> <p>Conclusion</p> <p>Our results suggest that mechanical compression reduces the capacity of the bronchial epithelium to close wounds, and is, in part, mediated by PGE<sub>2 </sub>and a compromised cytoskeleton.</p> |
| first_indexed | 2024-04-13T03:09:20Z |
| format | Article |
| id | doaj.art-00e36e4333594205a76ddf1f67ed8d2d |
| institution | Directory Open Access Journal |
| issn | 1465-9921 |
| language | English |
| last_indexed | 2024-04-13T03:09:20Z |
| publishDate | 2009-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-00e36e4333594205a76ddf1f67ed8d2d2022-12-22T03:05:08ZengBMCRespiratory Research1465-99212009-02-01101910.1186/1465-9921-10-9Mechanical compression attenuates normal human bronchial epithelial wound healingMalavia NikitaArold Stephen PGeorge Steven C<p>Abstract</p> <p>Background</p> <p>Airway narrowing associated with chronic asthma results in the transmission of injurious compressive forces to the bronchial epithelium and promotes the release of pro-inflammatory mediators and the denudation of the bronchial epithelium. While the individual effects of compression or denudation are well characterized, there is no data to elucidate how these cells respond to the application of mechanical compression in the presence of a compromised epithelial layer.</p> <p>Methods</p> <p>Accordingly, differentiated normal human bronchial epithelial cells were exposed to one of four conditions: 1) unperturbed control cells, 2) single scrape wound only, 3) static compression (6 hours of 30 cmH<sub>2</sub>O), and 4) 6 hours of static compression after a scrape wound. Following treatment, wound closure rate was recorded, media was assayed for mediator content and the cytoskeletal network was fluorescently labeled.</p> <p>Results</p> <p>We found that mechanical compression and scrape injury increase TGF-β2 and endothelin-1 secretion, while EGF content in the media is attenuated with both injury modes. The application of compression after a pre-existing scrape wound augmented these observations, and also decreased PGE<sub>2 </sub>media content. Compression stimulated depolymerization of the actin cytoskeleton and significantly attenuated wound healing. Closure rate was partially restored with the addition of exogenous PGE<sub>2</sub>, but not EGF.</p> <p>Conclusion</p> <p>Our results suggest that mechanical compression reduces the capacity of the bronchial epithelium to close wounds, and is, in part, mediated by PGE<sub>2 </sub>and a compromised cytoskeleton.</p>http://www.biomedcentral.com/1465-9921/10/9 |
| spellingShingle | Malavia Nikita Arold Stephen P George Steven C Mechanical compression attenuates normal human bronchial epithelial wound healing Respiratory Research |
| title | Mechanical compression attenuates normal human bronchial epithelial wound healing |
| title_full | Mechanical compression attenuates normal human bronchial epithelial wound healing |
| title_fullStr | Mechanical compression attenuates normal human bronchial epithelial wound healing |
| title_full_unstemmed | Mechanical compression attenuates normal human bronchial epithelial wound healing |
| title_short | Mechanical compression attenuates normal human bronchial epithelial wound healing |
| title_sort | mechanical compression attenuates normal human bronchial epithelial wound healing |
| url | http://www.biomedcentral.com/1465-9921/10/9 |
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