Blue and Long-Wave Ultraviolet Light Induce in vitro Neutrophil Extracellular Trap (NET) Formation
Neutrophil Extracellular Traps (NETs) are produced by neutrophilic granulocytes and consist of decondensed chromatin decorated with antimicrobial peptides. They defend the organism against intruders and are released upon various stimuli including pathogens, mediators of inflammation, or chemical tri...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.02428/full |
| _version_ | 1811263071500369920 |
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| author | Elsa Neubert Elsa Neubert Katharina Marie Bach Julia Busse Ivan Bogeski Michael P. Schön Michael P. Schön Sebastian Kruss Sebastian Kruss Luise Erpenbeck |
| author_facet | Elsa Neubert Elsa Neubert Katharina Marie Bach Julia Busse Ivan Bogeski Michael P. Schön Michael P. Schön Sebastian Kruss Sebastian Kruss Luise Erpenbeck |
| author_sort | Elsa Neubert |
| collection | DOAJ |
| description | Neutrophil Extracellular Traps (NETs) are produced by neutrophilic granulocytes and consist of decondensed chromatin decorated with antimicrobial peptides. They defend the organism against intruders and are released upon various stimuli including pathogens, mediators of inflammation, or chemical triggers. NET formation is also involved in inflammatory, cardiovascular, malignant diseases, and autoimmune disorders like rheumatoid arthritis, psoriasis, or systemic lupus erythematosus (SLE). In many autoimmune diseases like SLE or dermatomyositis, light of the ultraviolet-visible (UV-VIS) spectrum is well-known to trigger and aggravate disease severity. However, the underlying connection between NET formation, light exposure, and disease exacerbation remains elusive. We studied the effect of UVA (375 nm), blue (470 nm) and green (565 nm) light on NETosis in human neutrophils ex vivo. Our results show a dose- and wavelength-dependent induction of NETosis. Light-induced NETosis depended on the generation of extracellular reactive oxygen species (ROS) induced by riboflavin excitation and its subsequent reaction with tryptophan. The light-induced NETosis required both neutrophil elastase (NE) as well as myeloperoxidase (MPO) activation and induced histone citrullination. These findings suggest that NET formation as a response to light could be the hitherto missing link between elevated susceptibility to NET formation in autoimmune patients and photosensitivity for example in SLE and dermatomyositis patients. This novel connection could provide a clue for a deeper understanding of light-sensitive diseases in general and for the development of new pharmacological strategies to avoid disease exacerbation upon light exposure. |
| first_indexed | 2024-04-12T19:38:14Z |
| format | Article |
| id | doaj.art-00e4426662a7406c8081c2433d143845 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-12T19:38:14Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-00e4426662a7406c8081c2433d1438452022-12-22T03:19:09ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-10-011010.3389/fimmu.2019.02428476466Blue and Long-Wave Ultraviolet Light Induce in vitro Neutrophil Extracellular Trap (NET) FormationElsa Neubert0Elsa Neubert1Katharina Marie Bach2Julia Busse3Ivan Bogeski4Michael P. Schön5Michael P. Schön6Sebastian Kruss7Sebastian Kruss8Luise Erpenbeck9Department of Dermatology, Venereology and Allergology, University Medical Center Göttingen, Göttingen, GermanyInstitute of Physical Chemistry, Göttingen University, Göttingen, GermanyDepartment of Dermatology, Venereology and Allergology, University Medical Center Göttingen, Göttingen, GermanyDepartment of Dermatology, Venereology and Allergology, University Medical Center Göttingen, Göttingen, GermanyInstitute of Cardiovascular Physiology, University Medical Center Göttingen, Göttingen, GermanyDepartment of Dermatology, Venereology and Allergology, University Medical Center Göttingen, Göttingen, GermanyLower Saxony Institute of Occupational Dermatology, University Medical Center Göttingen, Göttingen, GermanyInstitute of Physical Chemistry, Göttingen University, Göttingen, GermanyCenter for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), Göttingen, GermanyDepartment of Dermatology, Venereology and Allergology, University Medical Center Göttingen, Göttingen, GermanyNeutrophil Extracellular Traps (NETs) are produced by neutrophilic granulocytes and consist of decondensed chromatin decorated with antimicrobial peptides. They defend the organism against intruders and are released upon various stimuli including pathogens, mediators of inflammation, or chemical triggers. NET formation is also involved in inflammatory, cardiovascular, malignant diseases, and autoimmune disorders like rheumatoid arthritis, psoriasis, or systemic lupus erythematosus (SLE). In many autoimmune diseases like SLE or dermatomyositis, light of the ultraviolet-visible (UV-VIS) spectrum is well-known to trigger and aggravate disease severity. However, the underlying connection between NET formation, light exposure, and disease exacerbation remains elusive. We studied the effect of UVA (375 nm), blue (470 nm) and green (565 nm) light on NETosis in human neutrophils ex vivo. Our results show a dose- and wavelength-dependent induction of NETosis. Light-induced NETosis depended on the generation of extracellular reactive oxygen species (ROS) induced by riboflavin excitation and its subsequent reaction with tryptophan. The light-induced NETosis required both neutrophil elastase (NE) as well as myeloperoxidase (MPO) activation and induced histone citrullination. These findings suggest that NET formation as a response to light could be the hitherto missing link between elevated susceptibility to NET formation in autoimmune patients and photosensitivity for example in SLE and dermatomyositis patients. This novel connection could provide a clue for a deeper understanding of light-sensitive diseases in general and for the development of new pharmacological strategies to avoid disease exacerbation upon light exposure.https://www.frontiersin.org/article/10.3389/fimmu.2019.02428/fullneutrophilic granulocytesNET formationUV-Vis lightinflammationROS formationriboflavin |
| spellingShingle | Elsa Neubert Elsa Neubert Katharina Marie Bach Julia Busse Ivan Bogeski Michael P. Schön Michael P. Schön Sebastian Kruss Sebastian Kruss Luise Erpenbeck Blue and Long-Wave Ultraviolet Light Induce in vitro Neutrophil Extracellular Trap (NET) Formation Frontiers in Immunology neutrophilic granulocytes NET formation UV-Vis light inflammation ROS formation riboflavin |
| title | Blue and Long-Wave Ultraviolet Light Induce in vitro Neutrophil Extracellular Trap (NET) Formation |
| title_full | Blue and Long-Wave Ultraviolet Light Induce in vitro Neutrophil Extracellular Trap (NET) Formation |
| title_fullStr | Blue and Long-Wave Ultraviolet Light Induce in vitro Neutrophil Extracellular Trap (NET) Formation |
| title_full_unstemmed | Blue and Long-Wave Ultraviolet Light Induce in vitro Neutrophil Extracellular Trap (NET) Formation |
| title_short | Blue and Long-Wave Ultraviolet Light Induce in vitro Neutrophil Extracellular Trap (NET) Formation |
| title_sort | blue and long wave ultraviolet light induce in vitro neutrophil extracellular trap net formation |
| topic | neutrophilic granulocytes NET formation UV-Vis light inflammation ROS formation riboflavin |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.02428/full |
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