A Built-In CpG Adjuvant in RSV F Protein DNA Vaccine Drives a Th1 Polarized and Enhanced Protective Immune Response
Human respiratory syncytial virus (RSV) is the most significant cause of acute lower respiratory infection in children. However, there is no licensed vaccine available. Here, we investigated the effect of five or 20 copies of C-Class of CpG ODN (CpG-C) motif incorporated into a plasmid DNA vaccine e...
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| Format: | Article |
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MDPI AG
2018-01-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/10/1/38 |
| _version_ | 1828434073932005376 |
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| author | Yao Ma Yue-Ying Jiao Yun-Zhou Yu Nan Jiang Ying Hua Xiu-Juan Zhang Yuan-Hui Fu Xiang-Lei Peng Yan-Peng Zheng Larry J. Anderson Jin-Sheng He |
| author_facet | Yao Ma Yue-Ying Jiao Yun-Zhou Yu Nan Jiang Ying Hua Xiu-Juan Zhang Yuan-Hui Fu Xiang-Lei Peng Yan-Peng Zheng Larry J. Anderson Jin-Sheng He |
| author_sort | Yao Ma |
| collection | DOAJ |
| description | Human respiratory syncytial virus (RSV) is the most significant cause of acute lower respiratory infection in children. However, there is no licensed vaccine available. Here, we investigated the effect of five or 20 copies of C-Class of CpG ODN (CpG-C) motif incorporated into a plasmid DNA vaccine encoding RSV fusion (F) glycoprotein on the vaccine-induced immune response. The addition of CpG-C motif enhanced serum binding and virus-neutralizing antibody responses in BALB/c mice immunized with the DNA vaccines. Moreover, mice vaccinated with CpG-modified vaccines, especially with the higher 20 copies, resulted in an enhanced shift toward a Th1-biased antibody and T-cell response, a decrease in pulmonary pathology and virus replication, and a decrease in weight loss after RSV challenge. This study suggests that CpG-C motif, cloned into the backbone of DNA vaccine encoding RSV F glycoprotein, functions as a built-in adjuvant capable of improving the efficacy of DNA vaccine against RSV infection. |
| first_indexed | 2024-12-10T18:45:07Z |
| format | Article |
| id | doaj.art-00e61767c93a4250ae7e433526021d27 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-10T18:45:07Z |
| publishDate | 2018-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-00e61767c93a4250ae7e433526021d272022-12-22T01:37:31ZengMDPI AGViruses1999-49152018-01-011013810.3390/v10010038v10010038A Built-In CpG Adjuvant in RSV F Protein DNA Vaccine Drives a Th1 Polarized and Enhanced Protective Immune ResponseYao Ma0Yue-Ying Jiao1Yun-Zhou Yu2Nan Jiang3Ying Hua4Xiu-Juan Zhang5Yuan-Hui Fu6Xiang-Lei Peng7Yan-Peng Zheng8Larry J. Anderson9Jin-Sheng He10College of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, ChinaCollege of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, ChinaBeijing Institute of Biotechnology, Beijing 100071, ChinaCollege of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, ChinaCollege of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, ChinaCollege of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, ChinaCollege of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, ChinaCollege of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, ChinaCollege of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, ChinaDepartment of Pediatrics, Emory University and Children’s Healthcare of Atlanta, Atlanta, GA 30322, USACollege of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, ChinaHuman respiratory syncytial virus (RSV) is the most significant cause of acute lower respiratory infection in children. However, there is no licensed vaccine available. Here, we investigated the effect of five or 20 copies of C-Class of CpG ODN (CpG-C) motif incorporated into a plasmid DNA vaccine encoding RSV fusion (F) glycoprotein on the vaccine-induced immune response. The addition of CpG-C motif enhanced serum binding and virus-neutralizing antibody responses in BALB/c mice immunized with the DNA vaccines. Moreover, mice vaccinated with CpG-modified vaccines, especially with the higher 20 copies, resulted in an enhanced shift toward a Th1-biased antibody and T-cell response, a decrease in pulmonary pathology and virus replication, and a decrease in weight loss after RSV challenge. This study suggests that CpG-C motif, cloned into the backbone of DNA vaccine encoding RSV F glycoprotein, functions as a built-in adjuvant capable of improving the efficacy of DNA vaccine against RSV infection.http://www.mdpi.com/1999-4915/10/1/38human respiratory syncytial virusCpG oligodeoxynucleotideDNA vaccinebuilt-in adjuvantprotective immunity |
| spellingShingle | Yao Ma Yue-Ying Jiao Yun-Zhou Yu Nan Jiang Ying Hua Xiu-Juan Zhang Yuan-Hui Fu Xiang-Lei Peng Yan-Peng Zheng Larry J. Anderson Jin-Sheng He A Built-In CpG Adjuvant in RSV F Protein DNA Vaccine Drives a Th1 Polarized and Enhanced Protective Immune Response Viruses human respiratory syncytial virus CpG oligodeoxynucleotide DNA vaccine built-in adjuvant protective immunity |
| title | A Built-In CpG Adjuvant in RSV F Protein DNA Vaccine Drives a Th1 Polarized and Enhanced Protective Immune Response |
| title_full | A Built-In CpG Adjuvant in RSV F Protein DNA Vaccine Drives a Th1 Polarized and Enhanced Protective Immune Response |
| title_fullStr | A Built-In CpG Adjuvant in RSV F Protein DNA Vaccine Drives a Th1 Polarized and Enhanced Protective Immune Response |
| title_full_unstemmed | A Built-In CpG Adjuvant in RSV F Protein DNA Vaccine Drives a Th1 Polarized and Enhanced Protective Immune Response |
| title_short | A Built-In CpG Adjuvant in RSV F Protein DNA Vaccine Drives a Th1 Polarized and Enhanced Protective Immune Response |
| title_sort | built in cpg adjuvant in rsv f protein dna vaccine drives a th1 polarized and enhanced protective immune response |
| topic | human respiratory syncytial virus CpG oligodeoxynucleotide DNA vaccine built-in adjuvant protective immunity |
| url | http://www.mdpi.com/1999-4915/10/1/38 |
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