Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice
To determine whether cholera toxin B subunit and active peptide from shark liver (CTB-APSL) fusion protein plays a role in treatment of type 2 diabetic mice, the CTB-APSL gene was cloned and expressed in silkworm (Bombyx mori) baculovirus expression vector system (BEVS), then the fusion protein was...
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| Format: | Article |
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MDPI AG
2014-03-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/12/3/1512 |
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| author | Yunlong Liu Zhangzhao Gao Qingtuo Guo Tao Wang Conger Lu Ying Chen Qing Sheng Jian Chen Zuoming Nie Yaozhou Zhang Wutong Wu Zhengbing Lv Jianhong Shu |
| author_facet | Yunlong Liu Zhangzhao Gao Qingtuo Guo Tao Wang Conger Lu Ying Chen Qing Sheng Jian Chen Zuoming Nie Yaozhou Zhang Wutong Wu Zhengbing Lv Jianhong Shu |
| author_sort | Yunlong Liu |
| collection | DOAJ |
| description | To determine whether cholera toxin B subunit and active peptide from shark liver (CTB-APSL) fusion protein plays a role in treatment of type 2 diabetic mice, the CTB-APSL gene was cloned and expressed in silkworm (Bombyx mori) baculovirus expression vector system (BEVS), then the fusion protein was orally administrated at a dose of 100 mg/kg for five weeks in diabetic mice. The results demonstrated that the oral administration of CTB-APSL fusion protein can effectively reduce the levels of both fasting blood glucose (FBG) and glycosylated hemoglobin (GHb), promote insulin secretion and improve insulin resistance, significantly improve lipid metabolism, reduce triglycerides (TG), total cholesterol (TC) and low density lipoprotein (LDL) levels and increase high density lipoprotein (HDL) levels, as well as effectively improve the inflammatory response of type 2 diabetic mice through the reduction of the levels of inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Histopathology shows that the fusion protein can significantly repair damaged pancreatic tissue in type 2 diabetic mice, significantly improve hepatic steatosis and hepatic cell cloudy swelling, reduce the content of lipid droplets in type 2 diabetic mice, effectively inhibit renal interstitial inflammatory cells invasion and improve renal tubular epithelial cell nucleus pyknosis, thus providing an experimental basis for the development of a new type of oral therapy for type 2 diabetes. |
| first_indexed | 2024-04-11T13:16:35Z |
| format | Article |
| id | doaj.art-00eb688141ed4c2987c5603b7fcd8a6d |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T13:16:35Z |
| publishDate | 2014-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-00eb688141ed4c2987c5603b7fcd8a6d2022-12-22T04:22:22ZengMDPI AGMarine Drugs1660-33972014-03-011231512152910.3390/md12031512md12031512Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic MiceYunlong Liu0Zhangzhao Gao1Qingtuo Guo2Tao Wang3Conger Lu4Ying Chen5Qing Sheng6Jian Chen7Zuoming Nie8Yaozhou Zhang9Wutong Wu10Zhengbing Lv11Jianhong Shu12College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaTo determine whether cholera toxin B subunit and active peptide from shark liver (CTB-APSL) fusion protein plays a role in treatment of type 2 diabetic mice, the CTB-APSL gene was cloned and expressed in silkworm (Bombyx mori) baculovirus expression vector system (BEVS), then the fusion protein was orally administrated at a dose of 100 mg/kg for five weeks in diabetic mice. The results demonstrated that the oral administration of CTB-APSL fusion protein can effectively reduce the levels of both fasting blood glucose (FBG) and glycosylated hemoglobin (GHb), promote insulin secretion and improve insulin resistance, significantly improve lipid metabolism, reduce triglycerides (TG), total cholesterol (TC) and low density lipoprotein (LDL) levels and increase high density lipoprotein (HDL) levels, as well as effectively improve the inflammatory response of type 2 diabetic mice through the reduction of the levels of inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Histopathology shows that the fusion protein can significantly repair damaged pancreatic tissue in type 2 diabetic mice, significantly improve hepatic steatosis and hepatic cell cloudy swelling, reduce the content of lipid droplets in type 2 diabetic mice, effectively inhibit renal interstitial inflammatory cells invasion and improve renal tubular epithelial cell nucleus pyknosis, thus providing an experimental basis for the development of a new type of oral therapy for type 2 diabetes.http://www.mdpi.com/1660-3397/12/3/1512active peptide from shark livercholera toxin B subunitBombyx mori pupaetype 2 diabetes mellitusoral administration |
| spellingShingle | Yunlong Liu Zhangzhao Gao Qingtuo Guo Tao Wang Conger Lu Ying Chen Qing Sheng Jian Chen Zuoming Nie Yaozhou Zhang Wutong Wu Zhengbing Lv Jianhong Shu Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice Marine Drugs active peptide from shark liver cholera toxin B subunit Bombyx mori pupae type 2 diabetes mellitus oral administration |
| title | Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice |
| title_full | Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice |
| title_fullStr | Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice |
| title_full_unstemmed | Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice |
| title_short | Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice |
| title_sort | anti diabetic effects of ctb apsl fusion protein in type 2 diabetic mice |
| topic | active peptide from shark liver cholera toxin B subunit Bombyx mori pupae type 2 diabetes mellitus oral administration |
| url | http://www.mdpi.com/1660-3397/12/3/1512 |
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