Viability, Storage Stability and In Vitro Gastrointestinal Tolerance of <em>Lactiplantibacillus plantarum</em> Grown in Model Sugar Systems with Inulin and Fructooligosaccharide Supplementation
This study aims to investigate the effects of inulin and fructooligosaccharides (FOS) supplementation on the viability, storage stability, and in vitro gastrointestinal tolerance of <i>Lactiplantibacillus plantarum</i> in different sugar systems using 24 h growth and 10 days survival stu...
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MDPI AG
2021-11-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/7/4/259 |
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| author | Priyanka Parhi Keang Peng Song Wee Sim Choo |
| author_facet | Priyanka Parhi Keang Peng Song Wee Sim Choo |
| author_sort | Priyanka Parhi |
| collection | DOAJ |
| description | This study aims to investigate the effects of inulin and fructooligosaccharides (FOS) supplementation on the viability, storage stability, and in vitro gastrointestinal tolerance of <i>Lactiplantibacillus plantarum</i> in different sugar systems using 24 h growth and 10 days survival studies at 37 °C, inulin, and FOS (0%, 0.5%, 1%, 2%, 3% and 4%) supplementation in 2%, 3%, and 4% glucose, fructose, lactose, and sucrose systems. Based on the highest percentage increase in growth index, sucrose and lactose were more suitable sugar substrates for inulin and FOS supplementation. In survival studies, based on cell viability, inulin supplementation showed a better protective effect than FOS in 3% and 4% sucrose and lactose systems. Four selected sucrose and lactose systems supplemented with inulin and FOS were used in a 12-week storage stability study at 4 °C. Inulin (3%, 4%) and FOS (2%, 4%) supplementation in sucrose and lactose systems greatly enhanced the refrigerated storage stability of <i>L. plantarum</i>. In the gastrointestinal tolerance study, an increase in the bacterial survival rate (%) showed that the supplementation of FOS in lactose and sucrose systems improved the storage viability of <i>L. plantarum.</i> Both inulin and FOS supplementation in sucrose and lactose systems improved the hydrophobicity, auto-aggregation, co-aggregation ability of <i>L. plantarum</i> with <i>Escherichia coli</i> and <i>Enterococcus faecalis</i>. |
| first_indexed | 2024-03-10T04:09:45Z |
| format | Article |
| id | doaj.art-00f6e68b462b46159570e31a485420bf |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T04:09:45Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-00f6e68b462b46159570e31a485420bf2023-11-23T08:13:08ZengMDPI AGFermentation2311-56372021-11-017425910.3390/fermentation7040259Viability, Storage Stability and In Vitro Gastrointestinal Tolerance of <em>Lactiplantibacillus plantarum</em> Grown in Model Sugar Systems with Inulin and Fructooligosaccharide SupplementationPriyanka Parhi0Keang Peng Song1Wee Sim Choo2School of Science, Monash University Malaysia, Subang Jaya 47500, Selangor, MalaysiaSchool of Science, Monash University Malaysia, Subang Jaya 47500, Selangor, MalaysiaSchool of Science, Monash University Malaysia, Subang Jaya 47500, Selangor, MalaysiaThis study aims to investigate the effects of inulin and fructooligosaccharides (FOS) supplementation on the viability, storage stability, and in vitro gastrointestinal tolerance of <i>Lactiplantibacillus plantarum</i> in different sugar systems using 24 h growth and 10 days survival studies at 37 °C, inulin, and FOS (0%, 0.5%, 1%, 2%, 3% and 4%) supplementation in 2%, 3%, and 4% glucose, fructose, lactose, and sucrose systems. Based on the highest percentage increase in growth index, sucrose and lactose were more suitable sugar substrates for inulin and FOS supplementation. In survival studies, based on cell viability, inulin supplementation showed a better protective effect than FOS in 3% and 4% sucrose and lactose systems. Four selected sucrose and lactose systems supplemented with inulin and FOS were used in a 12-week storage stability study at 4 °C. Inulin (3%, 4%) and FOS (2%, 4%) supplementation in sucrose and lactose systems greatly enhanced the refrigerated storage stability of <i>L. plantarum</i>. In the gastrointestinal tolerance study, an increase in the bacterial survival rate (%) showed that the supplementation of FOS in lactose and sucrose systems improved the storage viability of <i>L. plantarum.</i> Both inulin and FOS supplementation in sucrose and lactose systems improved the hydrophobicity, auto-aggregation, co-aggregation ability of <i>L. plantarum</i> with <i>Escherichia coli</i> and <i>Enterococcus faecalis</i>.https://www.mdpi.com/2311-5637/7/4/259glucosefructoselactoseprebioticprobioticsucrose |
| spellingShingle | Priyanka Parhi Keang Peng Song Wee Sim Choo Viability, Storage Stability and In Vitro Gastrointestinal Tolerance of <em>Lactiplantibacillus plantarum</em> Grown in Model Sugar Systems with Inulin and Fructooligosaccharide Supplementation Fermentation glucose fructose lactose prebiotic probiotic sucrose |
| title | Viability, Storage Stability and In Vitro Gastrointestinal Tolerance of <em>Lactiplantibacillus plantarum</em> Grown in Model Sugar Systems with Inulin and Fructooligosaccharide Supplementation |
| title_full | Viability, Storage Stability and In Vitro Gastrointestinal Tolerance of <em>Lactiplantibacillus plantarum</em> Grown in Model Sugar Systems with Inulin and Fructooligosaccharide Supplementation |
| title_fullStr | Viability, Storage Stability and In Vitro Gastrointestinal Tolerance of <em>Lactiplantibacillus plantarum</em> Grown in Model Sugar Systems with Inulin and Fructooligosaccharide Supplementation |
| title_full_unstemmed | Viability, Storage Stability and In Vitro Gastrointestinal Tolerance of <em>Lactiplantibacillus plantarum</em> Grown in Model Sugar Systems with Inulin and Fructooligosaccharide Supplementation |
| title_short | Viability, Storage Stability and In Vitro Gastrointestinal Tolerance of <em>Lactiplantibacillus plantarum</em> Grown in Model Sugar Systems with Inulin and Fructooligosaccharide Supplementation |
| title_sort | viability storage stability and in vitro gastrointestinal tolerance of em lactiplantibacillus plantarum em grown in model sugar systems with inulin and fructooligosaccharide supplementation |
| topic | glucose fructose lactose prebiotic probiotic sucrose |
| url | https://www.mdpi.com/2311-5637/7/4/259 |
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