Evaluation of a Colombian oregano oil (Lippia origanoides Kunth) and a novel yeast product from Pichia guilliermondii, alone or in combination, on rumen fermentation, methanogenesis and the microbiome in the rumen simulation technique
In this study, Lippia origanoides Kunth, native to the Alto Patía region in Southwest Colombia, and Pichia guilliermondii LV196, an inactivated yeast from the germplasm bank of Agrosavia (Colombian Agricultural Research Corporation), alone or combined, were tested for their long-term effect on rumen...
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| Format: | Article |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Animal Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fanim.2022.951789/full |
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| author | Eva Ramos-Morales Eva Ramos-Morales Emily Bolton Laura Lyons David Carreño Eleanor Jones Olga Mayorga Claudia Ariza-Nieto Charles James Newbold Charles James Newbold |
| author_facet | Eva Ramos-Morales Eva Ramos-Morales Emily Bolton Laura Lyons David Carreño Eleanor Jones Olga Mayorga Claudia Ariza-Nieto Charles James Newbold Charles James Newbold |
| author_sort | Eva Ramos-Morales |
| collection | DOAJ |
| description | In this study, Lippia origanoides Kunth, native to the Alto Patía region in Southwest Colombia, and Pichia guilliermondii LV196, an inactivated yeast from the germplasm bank of Agrosavia (Colombian Agricultural Research Corporation), alone or combined, were tested for their long-term effect on rumen fermentation and methanogenesis whilst also characterising their effect on bacterial and methanogen communities. Whereas essential oils act through selective inhibition of microbial groups, yeasts are thought to work through the selective stimulation of key microbes in the rumen. We hypothesized that yeast supplementation could modulate the antimicrobial effect of a high thymol-containing oregano oil, allowing a more efficient feed utilization whilst decreasing methane production. When added to a rumen simulating fermentor (RUSITEC), L. origanoides Kunth at 132 µL/d had a detrimental effect on rumen fermentation which was accompanied by a reduction in the relative abundance of protozoa and fungi and a profound impact on the bacterial and archaeal communities. P. guilliermondii LV196 at 0.5 g/L, however, had no effect on fermentation parameters or nutrient utilization, and neither changes in microbial abundances or in the structure of bacterial and archaeal communities were observed. P. guilliermondii LV196 did not stimulate microbial numbers nor activity and, consequently we could not test whether it could have counterbalanced the antimicrobial effect of the essential oil. Future studies need to both investigate lower levels of essential oil addition, but also to re-examine the effects of P. guillermondii in the rumen and/or to replace it with other yeast of known biological activity when combined with oils extracted from L. origanoides Kunth. |
| first_indexed | 2024-12-10T09:36:13Z |
| format | Article |
| id | doaj.art-00f8f743eeca4b66be578947b640929a |
| institution | Directory Open Access Journal |
| issn | 2673-6225 |
| language | English |
| last_indexed | 2024-12-10T09:36:13Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Animal Science |
| spelling | doaj.art-00f8f743eeca4b66be578947b640929a2022-12-22T01:54:10ZengFrontiers Media S.A.Frontiers in Animal Science2673-62252022-07-01310.3389/fanim.2022.951789951789Evaluation of a Colombian oregano oil (Lippia origanoides Kunth) and a novel yeast product from Pichia guilliermondii, alone or in combination, on rumen fermentation, methanogenesis and the microbiome in the rumen simulation techniqueEva Ramos-Morales0Eva Ramos-Morales1Emily Bolton2Laura Lyons3David Carreño4Eleanor Jones5Olga Mayorga6Claudia Ariza-Nieto7Charles James Newbold8Charles James Newbold9Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, United KingdomScotland’s Rural College, Edinburgh, United KingdomInstitute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, United KingdomInstitute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, United KingdomInstitute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, United KingdomInstitute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, United KingdomC.I. Tibaitatá, Agrosavia, Bogotá, ColombiaC.I. Tibaitatá, Agrosavia, Bogotá, ColombiaInstitute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, United KingdomScotland’s Rural College, Edinburgh, United KingdomIn this study, Lippia origanoides Kunth, native to the Alto Patía region in Southwest Colombia, and Pichia guilliermondii LV196, an inactivated yeast from the germplasm bank of Agrosavia (Colombian Agricultural Research Corporation), alone or combined, were tested for their long-term effect on rumen fermentation and methanogenesis whilst also characterising their effect on bacterial and methanogen communities. Whereas essential oils act through selective inhibition of microbial groups, yeasts are thought to work through the selective stimulation of key microbes in the rumen. We hypothesized that yeast supplementation could modulate the antimicrobial effect of a high thymol-containing oregano oil, allowing a more efficient feed utilization whilst decreasing methane production. When added to a rumen simulating fermentor (RUSITEC), L. origanoides Kunth at 132 µL/d had a detrimental effect on rumen fermentation which was accompanied by a reduction in the relative abundance of protozoa and fungi and a profound impact on the bacterial and archaeal communities. P. guilliermondii LV196 at 0.5 g/L, however, had no effect on fermentation parameters or nutrient utilization, and neither changes in microbial abundances or in the structure of bacterial and archaeal communities were observed. P. guilliermondii LV196 did not stimulate microbial numbers nor activity and, consequently we could not test whether it could have counterbalanced the antimicrobial effect of the essential oil. Future studies need to both investigate lower levels of essential oil addition, but also to re-examine the effects of P. guillermondii in the rumen and/or to replace it with other yeast of known biological activity when combined with oils extracted from L. origanoides Kunth.https://www.frontiersin.org/articles/10.3389/fanim.2022.951789/fullLippiamethanemicrobiomeoregano oilPichiarumen fermentation |
| spellingShingle | Eva Ramos-Morales Eva Ramos-Morales Emily Bolton Laura Lyons David Carreño Eleanor Jones Olga Mayorga Claudia Ariza-Nieto Charles James Newbold Charles James Newbold Evaluation of a Colombian oregano oil (Lippia origanoides Kunth) and a novel yeast product from Pichia guilliermondii, alone or in combination, on rumen fermentation, methanogenesis and the microbiome in the rumen simulation technique Frontiers in Animal Science Lippia methane microbiome oregano oil Pichia rumen fermentation |
| title | Evaluation of a Colombian oregano oil (Lippia origanoides Kunth) and a novel yeast product from Pichia guilliermondii, alone or in combination, on rumen fermentation, methanogenesis and the microbiome in the rumen simulation technique |
| title_full | Evaluation of a Colombian oregano oil (Lippia origanoides Kunth) and a novel yeast product from Pichia guilliermondii, alone or in combination, on rumen fermentation, methanogenesis and the microbiome in the rumen simulation technique |
| title_fullStr | Evaluation of a Colombian oregano oil (Lippia origanoides Kunth) and a novel yeast product from Pichia guilliermondii, alone or in combination, on rumen fermentation, methanogenesis and the microbiome in the rumen simulation technique |
| title_full_unstemmed | Evaluation of a Colombian oregano oil (Lippia origanoides Kunth) and a novel yeast product from Pichia guilliermondii, alone or in combination, on rumen fermentation, methanogenesis and the microbiome in the rumen simulation technique |
| title_short | Evaluation of a Colombian oregano oil (Lippia origanoides Kunth) and a novel yeast product from Pichia guilliermondii, alone or in combination, on rumen fermentation, methanogenesis and the microbiome in the rumen simulation technique |
| title_sort | evaluation of a colombian oregano oil lippia origanoides kunth and a novel yeast product from pichia guilliermondii alone or in combination on rumen fermentation methanogenesis and the microbiome in the rumen simulation technique |
| topic | Lippia methane microbiome oregano oil Pichia rumen fermentation |
| url | https://www.frontiersin.org/articles/10.3389/fanim.2022.951789/full |
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