Functions of retinal astrocytes and Müller cells in mammalian myopia
Abstract Background Changes in the retina and choroid blood vessels are regularly observed in myopia. However, if the retinal glial cells, which directly contact blood vessels, play a role in mammalian myopia is unknown. We aimed to explore the potential role and mechanism of retinal glial cells in...
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Ophthalmology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12886-022-02643-0 |
| _version_ | 1828119838423252992 |
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| author | Xuhong Zhang Xin Yu Yingying Wen Le Jin Liyue Zhang Hong Zhu Dongyan Zhang Chen Xie Dongyu Guo Jianping Tong Ye Shen |
| author_facet | Xuhong Zhang Xin Yu Yingying Wen Le Jin Liyue Zhang Hong Zhu Dongyan Zhang Chen Xie Dongyu Guo Jianping Tong Ye Shen |
| author_sort | Xuhong Zhang |
| collection | DOAJ |
| description | Abstract Background Changes in the retina and choroid blood vessels are regularly observed in myopia. However, if the retinal glial cells, which directly contact blood vessels, play a role in mammalian myopia is unknown. We aimed to explore the potential role and mechanism of retinal glial cells in form deprived myopia. Methods We adapted the mice form-deprivation myopia model by covering the right eye and left the left eye open for control, measured the ocular structure with anterior segment optical coherence tomography, evaluated changes in the morphology and distribution of retinal glial cells by fluorescence staining and western blotting; we also searched the online GEO databases to obtain relative gene lists and confirmed them in the form-deprivation myopia mouse retina at mRNA and protein level. Results Compared with the open eye, the ocular axial length (3.54 ± 0.006 mm v.s. 3.48 ± 0.004 mm, p = 0.027) and vitreous chamber depth (3.07 ± 0.005 mm v.s. 2.98 ± 0.006 mm, p = 0.007) in the covered eye became longer. Both glial fibrillary acidic protein and excitatory amino acid transporters 4 elevated. There were 12 common pathways in human myopia and anoxic astrocytes. The key proteins were also highly relevant to atropine target proteins. In mice, two common pathways were found in myopia and anoxic Müller cells. Seven main genes and four key proteins were significantly changed in the mice form-deprivation myopia retinas. Conclusion Retinal astrocytes and Müller cells were activated in myopia. They may response to stimuli and secretory acting factors, and might be a valid target for atropine. |
| first_indexed | 2024-04-11T13:55:31Z |
| format | Article |
| id | doaj.art-00f8fe2c6b1346a4b96153cc1afe6dc1 |
| institution | Directory Open Access Journal |
| issn | 1471-2415 |
| language | English |
| last_indexed | 2024-04-11T13:55:31Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Ophthalmology |
| spelling | doaj.art-00f8fe2c6b1346a4b96153cc1afe6dc12022-12-22T04:20:22ZengBMCBMC Ophthalmology1471-24152022-11-0122111510.1186/s12886-022-02643-0Functions of retinal astrocytes and Müller cells in mammalian myopiaXuhong Zhang0Xin Yu1Yingying Wen2Le Jin3Liyue Zhang4Hong Zhu5Dongyan Zhang6Chen Xie7Dongyu Guo8Jianping Tong9Ye Shen10Ophthalmology department, the First Affiliated Hospital of Zhejiang UniversityOphthalmology department, the First Affiliated Hospital of Zhejiang UniversityOphthalmology department, the First Affiliated Hospital of Zhejiang UniversityOphthalmology department, the First Affiliated Hospital of Zhejiang UniversityOphthalmology department, the First Affiliated Hospital of Zhejiang UniversityOphthalmology department, the First Affiliated Hospital of Zhejiang UniversityOphthalmology department, the First Affiliated Hospital of Zhejiang UniversityOphthalmology department, the First Affiliated Hospital of Zhejiang UniversityOphthalmology department, the First Affiliated Hospital of Zhejiang UniversityOphthalmology department, the First Affiliated Hospital of Zhejiang UniversityOphthalmology department, the First Affiliated Hospital of Zhejiang UniversityAbstract Background Changes in the retina and choroid blood vessels are regularly observed in myopia. However, if the retinal glial cells, which directly contact blood vessels, play a role in mammalian myopia is unknown. We aimed to explore the potential role and mechanism of retinal glial cells in form deprived myopia. Methods We adapted the mice form-deprivation myopia model by covering the right eye and left the left eye open for control, measured the ocular structure with anterior segment optical coherence tomography, evaluated changes in the morphology and distribution of retinal glial cells by fluorescence staining and western blotting; we also searched the online GEO databases to obtain relative gene lists and confirmed them in the form-deprivation myopia mouse retina at mRNA and protein level. Results Compared with the open eye, the ocular axial length (3.54 ± 0.006 mm v.s. 3.48 ± 0.004 mm, p = 0.027) and vitreous chamber depth (3.07 ± 0.005 mm v.s. 2.98 ± 0.006 mm, p = 0.007) in the covered eye became longer. Both glial fibrillary acidic protein and excitatory amino acid transporters 4 elevated. There were 12 common pathways in human myopia and anoxic astrocytes. The key proteins were also highly relevant to atropine target proteins. In mice, two common pathways were found in myopia and anoxic Müller cells. Seven main genes and four key proteins were significantly changed in the mice form-deprivation myopia retinas. Conclusion Retinal astrocytes and Müller cells were activated in myopia. They may response to stimuli and secretory acting factors, and might be a valid target for atropine.https://doi.org/10.1186/s12886-022-02643-0Gene set enrichment analysisMyopiaAstrocyteMüller cellAtropine |
| spellingShingle | Xuhong Zhang Xin Yu Yingying Wen Le Jin Liyue Zhang Hong Zhu Dongyan Zhang Chen Xie Dongyu Guo Jianping Tong Ye Shen Functions of retinal astrocytes and Müller cells in mammalian myopia BMC Ophthalmology Gene set enrichment analysis Myopia Astrocyte Müller cell Atropine |
| title | Functions of retinal astrocytes and Müller cells in mammalian myopia |
| title_full | Functions of retinal astrocytes and Müller cells in mammalian myopia |
| title_fullStr | Functions of retinal astrocytes and Müller cells in mammalian myopia |
| title_full_unstemmed | Functions of retinal astrocytes and Müller cells in mammalian myopia |
| title_short | Functions of retinal astrocytes and Müller cells in mammalian myopia |
| title_sort | functions of retinal astrocytes and muller cells in mammalian myopia |
| topic | Gene set enrichment analysis Myopia Astrocyte Müller cell Atropine |
| url | https://doi.org/10.1186/s12886-022-02643-0 |
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