Bone regeneration using composite non-demineralized xenogenic dentin with beta-tricalcium phosphate in experimental alveolar cleft repair in a rabbit model
Abstract Background Alveolar cleft repair is performed via bone grafting procedure to restore the dental arch continuity. A suitable bone substitute materials should possess osteoinductive and osteoconductive properties, to promote new bone formation, along with a slowly resorbable scaffold that is...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-12-01
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| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12967-017-1369-3 |
| _version_ | 1818684073930915840 |
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| author | Mohammad Kamal Lars Andersson Rene Tolba Adel Al-Asfour Alexander K. Bartella Felix Gremse Stefanie Rosenhain Frank Hölzle Peter Kessler Bernd Lethaus |
| author_facet | Mohammad Kamal Lars Andersson Rene Tolba Adel Al-Asfour Alexander K. Bartella Felix Gremse Stefanie Rosenhain Frank Hölzle Peter Kessler Bernd Lethaus |
| author_sort | Mohammad Kamal |
| collection | DOAJ |
| description | Abstract Background Alveolar cleft repair is performed via bone grafting procedure to restore the dental arch continuity. A suitable bone substitute materials should possess osteoinductive and osteoconductive properties, to promote new bone formation, along with a slowly resorbable scaffold that is subsequently replaced with functionally viable bone. Calcium phosphate biomaterials have long proved their efficacy as bone replacement materials. Dentin in several forms has also demonstrated its possibility to be used as bone graft replacement material in several studies. The purpose of this study was to evaluate bone regeneration pattern and quantify bone formation after grafting pre-established experimental alveolar clefts defects model in rabbits using composite xenogenic dentin and β-TCP in comparison to β-TCP alone. Methods Unilateral alveolar cleft defects were created in 16 New Zealand rabbits according to previously described methodology. Alveolar clefts were allowed 8 weeks healing period. 8 defects were filled with β-TCP, whereas 8 defects filled with composite xenogenic dentin with β-TCP. Bone regeneration of the healed defects was compared at the 8 weeks after intervention. Quantification of bone formation was analyzed using micro-computed tomography (µCT) and histomorphometric analysis. Results µCT and histomorphometric analysis revealed that defects filled with composite dentin/β-TCP showed statistically higher bone volume fraction, bone mineral density and percentage residual graft volume when compared to β-TCP alone. An improved surgical handling of the composite dentin/β-TCP graft was also noted. Conclusions Composite xenogenic dentin/β-TCP putty expresses enhanced bone regeneration compared to β-TCP alone in the reconstruction of rabbit alveolar clefts defects. |
| first_indexed | 2024-12-17T10:44:50Z |
| format | Article |
| id | doaj.art-00f9c998e11140c2975d280258563ba5 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-17T10:44:50Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-00f9c998e11140c2975d280258563ba52022-12-21T21:52:09ZengBMCJournal of Translational Medicine1479-58762017-12-0115111310.1186/s12967-017-1369-3Bone regeneration using composite non-demineralized xenogenic dentin with beta-tricalcium phosphate in experimental alveolar cleft repair in a rabbit modelMohammad Kamal0Lars Andersson1Rene Tolba2Adel Al-Asfour3Alexander K. Bartella4Felix Gremse5Stefanie Rosenhain6Frank Hölzle7Peter Kessler8Bernd Lethaus9Department of Cranio-Maxillofacial Surgery and GROW School for Oncology and Developmental Biology, Maastricht University Medical CenterDepartment of Surgical Sciences, Health Sciences Center, Kuwait UniversityInstitute for Laboratory Animal Science and Experimental Surgery, RWTH Aachen UniversityDepartment of Surgical Sciences, Health Sciences Center, Kuwait UniversityDepartment of Oral and Maxillofacial Surgery, RWTH Aachen UniversityDepartment of Experimental Molecular Imaging, RWTH Aachen UniversityDepartment of Experimental Molecular Imaging, RWTH Aachen UniversityDepartment of Oral and Maxillofacial Surgery, RWTH Aachen UniversityDepartment of Cranio-Maxillofacial Surgery and GROW School for Oncology and Developmental Biology, Maastricht University Medical CenterDepartment of Oral and Maxillofacial Surgery, RWTH Aachen UniversityAbstract Background Alveolar cleft repair is performed via bone grafting procedure to restore the dental arch continuity. A suitable bone substitute materials should possess osteoinductive and osteoconductive properties, to promote new bone formation, along with a slowly resorbable scaffold that is subsequently replaced with functionally viable bone. Calcium phosphate biomaterials have long proved their efficacy as bone replacement materials. Dentin in several forms has also demonstrated its possibility to be used as bone graft replacement material in several studies. The purpose of this study was to evaluate bone regeneration pattern and quantify bone formation after grafting pre-established experimental alveolar clefts defects model in rabbits using composite xenogenic dentin and β-TCP in comparison to β-TCP alone. Methods Unilateral alveolar cleft defects were created in 16 New Zealand rabbits according to previously described methodology. Alveolar clefts were allowed 8 weeks healing period. 8 defects were filled with β-TCP, whereas 8 defects filled with composite xenogenic dentin with β-TCP. Bone regeneration of the healed defects was compared at the 8 weeks after intervention. Quantification of bone formation was analyzed using micro-computed tomography (µCT) and histomorphometric analysis. Results µCT and histomorphometric analysis revealed that defects filled with composite dentin/β-TCP showed statistically higher bone volume fraction, bone mineral density and percentage residual graft volume when compared to β-TCP alone. An improved surgical handling of the composite dentin/β-TCP graft was also noted. Conclusions Composite xenogenic dentin/β-TCP putty expresses enhanced bone regeneration compared to β-TCP alone in the reconstruction of rabbit alveolar clefts defects.http://link.springer.com/article/10.1186/s12967-017-1369-3Animal testingCleft lip and palateAlveolar cleftBone transplantationGraftingTissue-engineering |
| spellingShingle | Mohammad Kamal Lars Andersson Rene Tolba Adel Al-Asfour Alexander K. Bartella Felix Gremse Stefanie Rosenhain Frank Hölzle Peter Kessler Bernd Lethaus Bone regeneration using composite non-demineralized xenogenic dentin with beta-tricalcium phosphate in experimental alveolar cleft repair in a rabbit model Journal of Translational Medicine Animal testing Cleft lip and palate Alveolar cleft Bone transplantation Grafting Tissue-engineering |
| title | Bone regeneration using composite non-demineralized xenogenic dentin with beta-tricalcium phosphate in experimental alveolar cleft repair in a rabbit model |
| title_full | Bone regeneration using composite non-demineralized xenogenic dentin with beta-tricalcium phosphate in experimental alveolar cleft repair in a rabbit model |
| title_fullStr | Bone regeneration using composite non-demineralized xenogenic dentin with beta-tricalcium phosphate in experimental alveolar cleft repair in a rabbit model |
| title_full_unstemmed | Bone regeneration using composite non-demineralized xenogenic dentin with beta-tricalcium phosphate in experimental alveolar cleft repair in a rabbit model |
| title_short | Bone regeneration using composite non-demineralized xenogenic dentin with beta-tricalcium phosphate in experimental alveolar cleft repair in a rabbit model |
| title_sort | bone regeneration using composite non demineralized xenogenic dentin with beta tricalcium phosphate in experimental alveolar cleft repair in a rabbit model |
| topic | Animal testing Cleft lip and palate Alveolar cleft Bone transplantation Grafting Tissue-engineering |
| url | http://link.springer.com/article/10.1186/s12967-017-1369-3 |
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