The Small Molecule BIBR1532 Exerts Potential Anti-cancer Activities in Preclinical Models of Feline Oral Squamous Cell Carcinoma Through Inhibition of Telomerase Activity and Down-Regulation of TERT
Expression of telomerase reverse transcriptase (TERT) and telomerase activity (TA) is a main feature of cancer, contributing to cell immortalization by causing telomeres dysfunction. BIBR1532 is a potent telomerase inhibitor that showed potential anti-tumor activities in several types of cancer, by...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2020.620776/full |
| _version_ | 1818668411971960832 |
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| author | Gennaro Altamura Barbara degli Uberti Giorgio Galiero Giovanna De Luca Karen Power Luca Licenziato Paola Maiolino Giuseppe Borzacchiello |
| author_facet | Gennaro Altamura Barbara degli Uberti Giorgio Galiero Giovanna De Luca Karen Power Luca Licenziato Paola Maiolino Giuseppe Borzacchiello |
| author_sort | Gennaro Altamura |
| collection | DOAJ |
| description | Expression of telomerase reverse transcriptase (TERT) and telomerase activity (TA) is a main feature of cancer, contributing to cell immortalization by causing telomeres dysfunction. BIBR1532 is a potent telomerase inhibitor that showed potential anti-tumor activities in several types of cancer, by triggering replicative senescence and apoptosis. In a previous work, we detected, for the first time, TERT expression and TA in preclinical models of feline oral squamous cell carcinoma (FOSCC); therefore, we aimed at extending our investigation by testing the effects of treatment with BIBR1532, in order to explore the role of telomerase in this tumor and foreshadow the possibility of it being considered as a future therapeutic target. In the present study, treatment of FOSCC cell lines SCCF1, SCCF2, and SCCF3 with BIBR1532 resulted in successful inhibition of TA, with subsequent cell growth stoppage and decrease in cell viability. Molecular data showed that up-regulation of cell cycle inhibitor p21, unbalancing of Bax/Bcl-2 ratio, and down-regulation of survival gene Survivin were mostly involved in the observed cellular events. Moreover, BIBR1532 diminished the expression of TERT and its transcriptional activator cMyc, resulting in the down-regulation of epidermal growth factor receptor (EGFR), phospho-ERK/ERK ratio, and matrix metalloproteinases (MMPs)-1/-2 and−9, likely as a consequence of an impairment of TERT extra-telomeric functions. Taken together, our data suggest that BIBR1532 exerts multiple anti-cancer activities in FOSCC by inhibiting telomerase pathway and interfering with signaling routes involved in cell proliferation, cell survival, and invasion, paving the way for future translational studies aimed at evaluating its possible employment in the treatment of this severe tumor of cats. |
| first_indexed | 2024-12-17T06:35:54Z |
| format | Article |
| id | doaj.art-00fa258a6c5d49b4aa0a98ed59cacd08 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-17T06:35:54Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-00fa258a6c5d49b4aa0a98ed59cacd082022-12-21T22:00:01ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-01-01710.3389/fvets.2020.620776620776The Small Molecule BIBR1532 Exerts Potential Anti-cancer Activities in Preclinical Models of Feline Oral Squamous Cell Carcinoma Through Inhibition of Telomerase Activity and Down-Regulation of TERTGennaro Altamura0Barbara degli Uberti1Giorgio Galiero2Giovanna De Luca3Karen Power4Luca Licenziato5Paola Maiolino6Giuseppe Borzacchiello7Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, ItalyIstituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, ItalyIstituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, ItalyIstituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, ItalyDepartment of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, ItalyDepartment of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, ItalyDepartment of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, ItalyDepartment of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, ItalyExpression of telomerase reverse transcriptase (TERT) and telomerase activity (TA) is a main feature of cancer, contributing to cell immortalization by causing telomeres dysfunction. BIBR1532 is a potent telomerase inhibitor that showed potential anti-tumor activities in several types of cancer, by triggering replicative senescence and apoptosis. In a previous work, we detected, for the first time, TERT expression and TA in preclinical models of feline oral squamous cell carcinoma (FOSCC); therefore, we aimed at extending our investigation by testing the effects of treatment with BIBR1532, in order to explore the role of telomerase in this tumor and foreshadow the possibility of it being considered as a future therapeutic target. In the present study, treatment of FOSCC cell lines SCCF1, SCCF2, and SCCF3 with BIBR1532 resulted in successful inhibition of TA, with subsequent cell growth stoppage and decrease in cell viability. Molecular data showed that up-regulation of cell cycle inhibitor p21, unbalancing of Bax/Bcl-2 ratio, and down-regulation of survival gene Survivin were mostly involved in the observed cellular events. Moreover, BIBR1532 diminished the expression of TERT and its transcriptional activator cMyc, resulting in the down-regulation of epidermal growth factor receptor (EGFR), phospho-ERK/ERK ratio, and matrix metalloproteinases (MMPs)-1/-2 and−9, likely as a consequence of an impairment of TERT extra-telomeric functions. Taken together, our data suggest that BIBR1532 exerts multiple anti-cancer activities in FOSCC by inhibiting telomerase pathway and interfering with signaling routes involved in cell proliferation, cell survival, and invasion, paving the way for future translational studies aimed at evaluating its possible employment in the treatment of this severe tumor of cats.https://www.frontiersin.org/articles/10.3389/fvets.2020.620776/fullBIBR1532telomerase activityTERTEGFRMMPcancer |
| spellingShingle | Gennaro Altamura Barbara degli Uberti Giorgio Galiero Giovanna De Luca Karen Power Luca Licenziato Paola Maiolino Giuseppe Borzacchiello The Small Molecule BIBR1532 Exerts Potential Anti-cancer Activities in Preclinical Models of Feline Oral Squamous Cell Carcinoma Through Inhibition of Telomerase Activity and Down-Regulation of TERT Frontiers in Veterinary Science BIBR1532 telomerase activity TERT EGFR MMP cancer |
| title | The Small Molecule BIBR1532 Exerts Potential Anti-cancer Activities in Preclinical Models of Feline Oral Squamous Cell Carcinoma Through Inhibition of Telomerase Activity and Down-Regulation of TERT |
| title_full | The Small Molecule BIBR1532 Exerts Potential Anti-cancer Activities in Preclinical Models of Feline Oral Squamous Cell Carcinoma Through Inhibition of Telomerase Activity and Down-Regulation of TERT |
| title_fullStr | The Small Molecule BIBR1532 Exerts Potential Anti-cancer Activities in Preclinical Models of Feline Oral Squamous Cell Carcinoma Through Inhibition of Telomerase Activity and Down-Regulation of TERT |
| title_full_unstemmed | The Small Molecule BIBR1532 Exerts Potential Anti-cancer Activities in Preclinical Models of Feline Oral Squamous Cell Carcinoma Through Inhibition of Telomerase Activity and Down-Regulation of TERT |
| title_short | The Small Molecule BIBR1532 Exerts Potential Anti-cancer Activities in Preclinical Models of Feline Oral Squamous Cell Carcinoma Through Inhibition of Telomerase Activity and Down-Regulation of TERT |
| title_sort | small molecule bibr1532 exerts potential anti cancer activities in preclinical models of feline oral squamous cell carcinoma through inhibition of telomerase activity and down regulation of tert |
| topic | BIBR1532 telomerase activity TERT EGFR MMP cancer |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2020.620776/full |
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