Metadherin mRNA expression in hepatocellular carcinoma
Background: Metadherin (MTDH) has been known as an essential oncogene in carcinogenesis and tumor spread in several malignancies, via its effect on pathways of signal transduction. Objective: We aimed to evaluate the role of serum MTDH mRNA expression in the diagnosis of hepatocellular carcinoma (HC...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-10-01
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| Series: | Egyptian Journal of Medical Human Genetics |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110863018300223 |
| _version_ | 1828873654597844992 |
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| author | Nevein M. Al-sheikh Sally M. El-Hefnway Ahmed M. Abuamer Ashraf G. Dala |
| author_facet | Nevein M. Al-sheikh Sally M. El-Hefnway Ahmed M. Abuamer Ashraf G. Dala |
| author_sort | Nevein M. Al-sheikh |
| collection | DOAJ |
| description | Background: Metadherin (MTDH) has been known as an essential oncogene in carcinogenesis and tumor spread in several malignancies, via its effect on pathways of signal transduction. Objective: We aimed to evaluate the role of serum MTDH mRNA expression in the diagnosis of hepatocellular carcinoma (HCC) and to compare its expression levels with serum levels of Alpha-fetoprotein (AFP). Subjects & methods: A total of 150 subjects (90 HCC patients & 60 healthy volunteers) were enrolled in the current study. Serum MTDH mRNA relative expression was analyzed by Real Time PCR technique. Results: There was a significant statistical increase of serum MTDH mRNA expression in HCC group when compared to controls (P < 0.05). MTDH mRNA expression was significantly associated with clinicopathological data, advanced tumor stage and poor histological differentiation in HCC patients (p < 0.05). There was direct positive correlation between MTDH mRNA expression and serum AFP levels in HCC group (r = 0.445), P value = <0.05. ROC curve was used to verify the accuracy of MTDH mRNA expression and compare it with accuracy of serum AFP in HCC diagnosis; MTDH mRNA expression had higher accuracy (92%), sensitivity (91%) and specificity (93%) than AFP. Conclusion: MTDH mRNA is up-regulated in serum of HCC patients; MTDH may be considered as non-invasive biomarker for HCC diagnosis and it could replace serum AFP in HCC diagnosis as it had higher accuracy. Keywords: Biomarker, Expression, Hepatocellular carcinoma, Metadherin, Real Time PCR |
| first_indexed | 2024-12-13T07:20:44Z |
| format | Article |
| id | doaj.art-00fdcb722e62444db8d1d7299c0fef22 |
| institution | Directory Open Access Journal |
| issn | 1110-8630 |
| language | English |
| last_indexed | 2024-12-13T07:20:44Z |
| publishDate | 2018-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Medical Human Genetics |
| spelling | doaj.art-00fdcb722e62444db8d1d7299c0fef222022-12-21T23:55:26ZengSpringerOpenEgyptian Journal of Medical Human Genetics1110-86302018-10-01194391397Metadherin mRNA expression in hepatocellular carcinomaNevein M. Al-sheikh0Sally M. El-Hefnway1Ahmed M. Abuamer2Ashraf G. Dala3Molecular Biology and Medical Biochemistry Department, Faculty of Medicine – Menoufia University, EgyptMolecular Biology and Medical Biochemistry Department, Faculty of Medicine – Menoufia University, Egypt; Corresponding author.Hepatology Department, National Liver Institute – Menoufia University, EgyptInternal Medicine Department, Faculty of Medicine – Menoufia University, EgyptBackground: Metadherin (MTDH) has been known as an essential oncogene in carcinogenesis and tumor spread in several malignancies, via its effect on pathways of signal transduction. Objective: We aimed to evaluate the role of serum MTDH mRNA expression in the diagnosis of hepatocellular carcinoma (HCC) and to compare its expression levels with serum levels of Alpha-fetoprotein (AFP). Subjects & methods: A total of 150 subjects (90 HCC patients & 60 healthy volunteers) were enrolled in the current study. Serum MTDH mRNA relative expression was analyzed by Real Time PCR technique. Results: There was a significant statistical increase of serum MTDH mRNA expression in HCC group when compared to controls (P < 0.05). MTDH mRNA expression was significantly associated with clinicopathological data, advanced tumor stage and poor histological differentiation in HCC patients (p < 0.05). There was direct positive correlation between MTDH mRNA expression and serum AFP levels in HCC group (r = 0.445), P value = <0.05. ROC curve was used to verify the accuracy of MTDH mRNA expression and compare it with accuracy of serum AFP in HCC diagnosis; MTDH mRNA expression had higher accuracy (92%), sensitivity (91%) and specificity (93%) than AFP. Conclusion: MTDH mRNA is up-regulated in serum of HCC patients; MTDH may be considered as non-invasive biomarker for HCC diagnosis and it could replace serum AFP in HCC diagnosis as it had higher accuracy. Keywords: Biomarker, Expression, Hepatocellular carcinoma, Metadherin, Real Time PCRhttp://www.sciencedirect.com/science/article/pii/S1110863018300223 |
| spellingShingle | Nevein M. Al-sheikh Sally M. El-Hefnway Ahmed M. Abuamer Ashraf G. Dala Metadherin mRNA expression in hepatocellular carcinoma Egyptian Journal of Medical Human Genetics |
| title | Metadherin mRNA expression in hepatocellular carcinoma |
| title_full | Metadherin mRNA expression in hepatocellular carcinoma |
| title_fullStr | Metadherin mRNA expression in hepatocellular carcinoma |
| title_full_unstemmed | Metadherin mRNA expression in hepatocellular carcinoma |
| title_short | Metadherin mRNA expression in hepatocellular carcinoma |
| title_sort | metadherin mrna expression in hepatocellular carcinoma |
| url | http://www.sciencedirect.com/science/article/pii/S1110863018300223 |
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