Similarities in Virulence and Extended Spectrum Beta-Lactamase Gene Profiles among Cefotaxime-Resistant <i>Escherichia coli</i> Wastewater and Clinical Isolates
The World Health Organization has identified antibiotic resistance as one of the largest threats to human health and food security. In this study, we compared antibiotic resistance patterns between ESBL-producing <i>Escherichia coli</i> from human clinical diseases and cefotaxime-resista...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/11/2/260 |
| _version_ | 1797483304308441088 |
|---|---|
| author | Elizabeth Liedhegner Brandon Bojar Rachelle E. Beattie Caitlin Cahak Krassimira R. Hristova Troy Skwor |
| author_facet | Elizabeth Liedhegner Brandon Bojar Rachelle E. Beattie Caitlin Cahak Krassimira R. Hristova Troy Skwor |
| author_sort | Elizabeth Liedhegner |
| collection | DOAJ |
| description | The World Health Organization has identified antibiotic resistance as one of the largest threats to human health and food security. In this study, we compared antibiotic resistance patterns between ESBL-producing <i>Escherichia coli</i> from human clinical diseases and cefotaxime-resistant environmental strains, as well as their potential to be pathogenic. Antibiotic susceptibility was tested amongst clinical isolates (<i>n</i> = 11), hospital wastewater (<i>n</i> = 22), and urban wastewater (<i>n</i> = 36, both influent and treated effluents). Multi-drug resistance predominated (>70%) among hospitalwastewater and urban wastewater influent isolates. Interestingly, isolates from clinical and urban treated effluents showed similar multi-drug resistance rates (~50%). Most hospital wastewater isolates were Phylogroup A, while clinical isolates were predominately B2, with a more diverse phylogroup population in urban wastewater. ESBL characterization of cefotaxime-resistant populations identified <i>bla</i><sub>CTX-M-1</sub> subgroup as the most common, whereby <i>bla</i><sub>KPC</sub> was more associated with ceftazidime and ertapenem resistance. Whole-genome sequencing of a carbapenemase-producing hospital wastewater <i>E. coli</i> strain revealed plasmid-mediated <i>bla</i><sub>KPC-2.</sub> Among cefotaxime-resistant populations, over 60% of clinical and 30% of treated effluent <i>E. coli</i> encoded three or more virulence genes exhibiting a pathogenic potential. Together, the similarity among treated effluent <i>E. coli</i> populations and clinical strains suggest effluents could serve as a reservoir for future multi-drug resistant <i>E. coli</i> clinical infections. |
| first_indexed | 2024-03-09T22:46:03Z |
| format | Article |
| id | doaj.art-00fde741dd30427d87eae1cd8de489f2 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T22:46:03Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-00fde741dd30427d87eae1cd8de489f22023-11-23T18:29:11ZengMDPI AGAntibiotics2079-63822022-02-0111226010.3390/antibiotics11020260Similarities in Virulence and Extended Spectrum Beta-Lactamase Gene Profiles among Cefotaxime-Resistant <i>Escherichia coli</i> Wastewater and Clinical IsolatesElizabeth Liedhegner0Brandon Bojar1Rachelle E. Beattie2Caitlin Cahak3Krassimira R. Hristova4Troy Skwor5Department of Biomedical Sciences, College of Health Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USADepartment of Biomedical Sciences, College of Health Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USADepartment of Biological Sciences, Marquette University, Milwaukee, WI 53233, USAWisconsin Diagnostic Laboratories, Milwaukee, WI 53226, USADepartment of Biological Sciences, Marquette University, Milwaukee, WI 53233, USADepartment of Biomedical Sciences, College of Health Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USAThe World Health Organization has identified antibiotic resistance as one of the largest threats to human health and food security. In this study, we compared antibiotic resistance patterns between ESBL-producing <i>Escherichia coli</i> from human clinical diseases and cefotaxime-resistant environmental strains, as well as their potential to be pathogenic. Antibiotic susceptibility was tested amongst clinical isolates (<i>n</i> = 11), hospital wastewater (<i>n</i> = 22), and urban wastewater (<i>n</i> = 36, both influent and treated effluents). Multi-drug resistance predominated (>70%) among hospitalwastewater and urban wastewater influent isolates. Interestingly, isolates from clinical and urban treated effluents showed similar multi-drug resistance rates (~50%). Most hospital wastewater isolates were Phylogroup A, while clinical isolates were predominately B2, with a more diverse phylogroup population in urban wastewater. ESBL characterization of cefotaxime-resistant populations identified <i>bla</i><sub>CTX-M-1</sub> subgroup as the most common, whereby <i>bla</i><sub>KPC</sub> was more associated with ceftazidime and ertapenem resistance. Whole-genome sequencing of a carbapenemase-producing hospital wastewater <i>E. coli</i> strain revealed plasmid-mediated <i>bla</i><sub>KPC-2.</sub> Among cefotaxime-resistant populations, over 60% of clinical and 30% of treated effluent <i>E. coli</i> encoded three or more virulence genes exhibiting a pathogenic potential. Together, the similarity among treated effluent <i>E. coli</i> populations and clinical strains suggest effluents could serve as a reservoir for future multi-drug resistant <i>E. coli</i> clinical infections.https://www.mdpi.com/2079-6382/11/2/260wastewaterESBLKPCCTX-Mvirulenceuropathogenic |
| spellingShingle | Elizabeth Liedhegner Brandon Bojar Rachelle E. Beattie Caitlin Cahak Krassimira R. Hristova Troy Skwor Similarities in Virulence and Extended Spectrum Beta-Lactamase Gene Profiles among Cefotaxime-Resistant <i>Escherichia coli</i> Wastewater and Clinical Isolates Antibiotics wastewater ESBL KPC CTX-M virulence uropathogenic |
| title | Similarities in Virulence and Extended Spectrum Beta-Lactamase Gene Profiles among Cefotaxime-Resistant <i>Escherichia coli</i> Wastewater and Clinical Isolates |
| title_full | Similarities in Virulence and Extended Spectrum Beta-Lactamase Gene Profiles among Cefotaxime-Resistant <i>Escherichia coli</i> Wastewater and Clinical Isolates |
| title_fullStr | Similarities in Virulence and Extended Spectrum Beta-Lactamase Gene Profiles among Cefotaxime-Resistant <i>Escherichia coli</i> Wastewater and Clinical Isolates |
| title_full_unstemmed | Similarities in Virulence and Extended Spectrum Beta-Lactamase Gene Profiles among Cefotaxime-Resistant <i>Escherichia coli</i> Wastewater and Clinical Isolates |
| title_short | Similarities in Virulence and Extended Spectrum Beta-Lactamase Gene Profiles among Cefotaxime-Resistant <i>Escherichia coli</i> Wastewater and Clinical Isolates |
| title_sort | similarities in virulence and extended spectrum beta lactamase gene profiles among cefotaxime resistant i escherichia coli i wastewater and clinical isolates |
| topic | wastewater ESBL KPC CTX-M virulence uropathogenic |
| url | https://www.mdpi.com/2079-6382/11/2/260 |
| work_keys_str_mv | AT elizabethliedhegner similaritiesinvirulenceandextendedspectrumbetalactamasegeneprofilesamongcefotaximeresistantiescherichiacoliiwastewaterandclinicalisolates AT brandonbojar similaritiesinvirulenceandextendedspectrumbetalactamasegeneprofilesamongcefotaximeresistantiescherichiacoliiwastewaterandclinicalisolates AT rachelleebeattie similaritiesinvirulenceandextendedspectrumbetalactamasegeneprofilesamongcefotaximeresistantiescherichiacoliiwastewaterandclinicalisolates AT caitlincahak similaritiesinvirulenceandextendedspectrumbetalactamasegeneprofilesamongcefotaximeresistantiescherichiacoliiwastewaterandclinicalisolates AT krassimirarhristova similaritiesinvirulenceandextendedspectrumbetalactamasegeneprofilesamongcefotaximeresistantiescherichiacoliiwastewaterandclinicalisolates AT troyskwor similaritiesinvirulenceandextendedspectrumbetalactamasegeneprofilesamongcefotaximeresistantiescherichiacoliiwastewaterandclinicalisolates |