Bemisia tabaci Phylogenetic Groups in India and the Relative Transmission Efficacy of Tomato leaf curl Bangalore virus by an Indigenous and an Exotic Population
Bemisia tabaci adults from various host-plant species were collected from 31 regions across India. 266 B. tabaci samples were first screened by RAPD-PCR to examine molecular variability and to select individuals with different fingerprints. Host-plant and region of collection were then used to selec...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2012-02-01
|
| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311912600082 |
| _version_ | 1819001642172809216 |
|---|---|
| author | RV Chowda-Reddy M Kirankumar Susan E Seal V Muniyappa Girish B Valand MR Govindappa John Colvin |
| author_facet | RV Chowda-Reddy M Kirankumar Susan E Seal V Muniyappa Girish B Valand MR Govindappa John Colvin |
| author_sort | RV Chowda-Reddy |
| collection | DOAJ |
| description | Bemisia tabaci adults from various host-plant species were collected from 31 regions across India. 266 B. tabaci samples were first screened by RAPD-PCR to examine molecular variability and to select individuals with different fingerprints. Host-plant and region of collection were then used to select 25 individuals for PCR amplification and sequencing of their partial mitochondrial cytochrome oxidase subunit one (mtCOI) genes. Pairwise comparisons with mtCOI consensus sequences showed that the majority of these samples had <3.5% sequence divergence from groups currently termed Asia I, Asia II-5, Asia II-7, and Asia II-8. The biotype-B B. tabaci from India clustered into the Middle East-Asia Minor 1 group. A new group of B. tabaci from Coimbatore, collected from pumpkin, was related most closely to the Asia I group (6.2% sequence divergence from the consensus Asia I sequence). To increase our understanding of the epidemiology of tomato leaf curl disease (ToLCD) and the key B. tabaci genetic groups involved in virus spread, the indigenous Asia I and the exotic biotype-B population from South India were used to carry out transmission experiments using Tomato leaf curl Bangalore virus (ToLCBV). The acquisition access periods (AAP), inoculation access periods (IAP), latent periods (LP), and ToLCBV transmission efficiencies of the two populations were compared and the biotype-B had the more efficient transmission characteristics. These results are discussed in relation to recent changes in the epidemiology of tomato leaf curl disease in South India. |
| first_indexed | 2024-12-20T22:52:27Z |
| format | Article |
| id | doaj.art-01022d0e53c6453fa4b1d83bf438fd93 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-20T22:52:27Z |
| publishDate | 2012-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-01022d0e53c6453fa4b1d83bf438fd932022-12-21T19:24:12ZengElsevierJournal of Integrative Agriculture2095-31192012-02-01112235248Bemisia tabaci Phylogenetic Groups in India and the Relative Transmission Efficacy of Tomato leaf curl Bangalore virus by an Indigenous and an Exotic PopulationRV Chowda-Reddy0M Kirankumar1Susan E Seal2V Muniyappa3Girish B Valand4MR Govindappa5John Colvin6Agriculture, Health and Environment Group, Natural Resources Institute, University of Greenwich at Medway, Chatham Maritime, Kent, ME4 4TB, United Kingdom; Southern Crop Protection and Food Research Centre, London-N5V 4T3, Canada; RV Chowda-Reddy, Tel: +519-457-1470 (ext 284), Fax: +519-457-3997Department of Plant Pathology, University of Agricultural Sciences, Bangalore-560 065, IndiaAgriculture, Health and Environment Group, Natural Resources Institute, University of Greenwich at Medway, Chatham Maritime, Kent, ME4 4TB, United KingdomDepartment of Plant Pathology, University of Agricultural Sciences, Bangalore-560 065, IndiaDepartment of Plant Pathology, Gujarat Agricultural University, Anand 388110, Gujarat, IndiaDepartment of Plant Pathology, University of Agricultural Sciences, Bangalore-560 065, IndiaAgriculture, Health and Environment Group, Natural Resources Institute, University of Greenwich at Medway, Chatham Maritime, Kent, ME4 4TB, United KingdomBemisia tabaci adults from various host-plant species were collected from 31 regions across India. 266 B. tabaci samples were first screened by RAPD-PCR to examine molecular variability and to select individuals with different fingerprints. Host-plant and region of collection were then used to select 25 individuals for PCR amplification and sequencing of their partial mitochondrial cytochrome oxidase subunit one (mtCOI) genes. Pairwise comparisons with mtCOI consensus sequences showed that the majority of these samples had <3.5% sequence divergence from groups currently termed Asia I, Asia II-5, Asia II-7, and Asia II-8. The biotype-B B. tabaci from India clustered into the Middle East-Asia Minor 1 group. A new group of B. tabaci from Coimbatore, collected from pumpkin, was related most closely to the Asia I group (6.2% sequence divergence from the consensus Asia I sequence). To increase our understanding of the epidemiology of tomato leaf curl disease (ToLCD) and the key B. tabaci genetic groups involved in virus spread, the indigenous Asia I and the exotic biotype-B population from South India were used to carry out transmission experiments using Tomato leaf curl Bangalore virus (ToLCBV). The acquisition access periods (AAP), inoculation access periods (IAP), latent periods (LP), and ToLCBV transmission efficiencies of the two populations were compared and the biotype-B had the more efficient transmission characteristics. These results are discussed in relation to recent changes in the epidemiology of tomato leaf curl disease in South India.http://www.sciencedirect.com/science/article/pii/S2095311912600082begomovirusesmitochondrial cytochrome oxidasewhiteflyBemisia tabacivirus-vector relationshipsepidemiology |
| spellingShingle | RV Chowda-Reddy M Kirankumar Susan E Seal V Muniyappa Girish B Valand MR Govindappa John Colvin Bemisia tabaci Phylogenetic Groups in India and the Relative Transmission Efficacy of Tomato leaf curl Bangalore virus by an Indigenous and an Exotic Population Journal of Integrative Agriculture begomoviruses mitochondrial cytochrome oxidase whitefly Bemisia tabaci virus-vector relationships epidemiology |
| title | Bemisia tabaci Phylogenetic Groups in India and the Relative Transmission Efficacy of Tomato leaf curl Bangalore virus by an Indigenous and an Exotic Population |
| title_full | Bemisia tabaci Phylogenetic Groups in India and the Relative Transmission Efficacy of Tomato leaf curl Bangalore virus by an Indigenous and an Exotic Population |
| title_fullStr | Bemisia tabaci Phylogenetic Groups in India and the Relative Transmission Efficacy of Tomato leaf curl Bangalore virus by an Indigenous and an Exotic Population |
| title_full_unstemmed | Bemisia tabaci Phylogenetic Groups in India and the Relative Transmission Efficacy of Tomato leaf curl Bangalore virus by an Indigenous and an Exotic Population |
| title_short | Bemisia tabaci Phylogenetic Groups in India and the Relative Transmission Efficacy of Tomato leaf curl Bangalore virus by an Indigenous and an Exotic Population |
| title_sort | bemisia tabaci phylogenetic groups in india and the relative transmission efficacy of tomato leaf curl bangalore virus by an indigenous and an exotic population |
| topic | begomoviruses mitochondrial cytochrome oxidase whitefly Bemisia tabaci virus-vector relationships epidemiology |
| url | http://www.sciencedirect.com/science/article/pii/S2095311912600082 |
| work_keys_str_mv | AT rvchowdareddy bemisiatabaciphylogeneticgroupsinindiaandtherelativetransmissionefficacyoftomatoleafcurlbangalorevirusbyanindigenousandanexoticpopulation AT mkirankumar bemisiatabaciphylogeneticgroupsinindiaandtherelativetransmissionefficacyoftomatoleafcurlbangalorevirusbyanindigenousandanexoticpopulation AT susaneseal bemisiatabaciphylogeneticgroupsinindiaandtherelativetransmissionefficacyoftomatoleafcurlbangalorevirusbyanindigenousandanexoticpopulation AT vmuniyappa bemisiatabaciphylogeneticgroupsinindiaandtherelativetransmissionefficacyoftomatoleafcurlbangalorevirusbyanindigenousandanexoticpopulation AT girishbvaland bemisiatabaciphylogeneticgroupsinindiaandtherelativetransmissionefficacyoftomatoleafcurlbangalorevirusbyanindigenousandanexoticpopulation AT mrgovindappa bemisiatabaciphylogeneticgroupsinindiaandtherelativetransmissionefficacyoftomatoleafcurlbangalorevirusbyanindigenousandanexoticpopulation AT johncolvin bemisiatabaciphylogeneticgroupsinindiaandtherelativetransmissionefficacyoftomatoleafcurlbangalorevirusbyanindigenousandanexoticpopulation |